Transcriptome-proteomic analysis associated with resistance to wheat cyst nematode Heterodera filipjevi

Motamedi, Marzieh; Esfahani, Mehdi Nasr; Monazzah, Maryam; Faridan, Vachik Gorgy; Nasr-Esfahani, Arman; Ghadirzadeh, Elham

doi:10.1016/j.pmpp.2023.102024

Cited by 10 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enhanced WRKY6 induction is consistent with enhancement of our related marker genes especially with R1, PR5, and RGA29 genes, basically resistant to FOC for comparing and con rming the results of our research (Chand et al, 2016;Straley et al, 2021;Moatamedi et al, 2023). This resistance is of a broad spectrum which may be long-lasting, and is rarely complete with most of the evolving genes for disease inhibition (Ruth et al, 2017;Tehrani et al, 2020;Qalavand et al, 2023).…”

Section: Discussionsupporting

confidence: 84%

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Poursakhi,

Asadi-Gharneh,

Esfahani

et al. 2024

Preprint

View full text Add to dashboard Cite

Fusarium basal rot (FBR) is a serious disease of onion that causes significant losses globally. There are several control measures, of which planting of resistant FBR-cultivars is the most advantageous control option. Thus, to elucidate the involving mechanisms to FBR infection, we consequently carried out quantification changes in antioxidant enzymes activity including ROS (reactive oxygen species); superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT) in two resistant onion genotypes ‘Saba’ and ‘Saba – HS’; and susceptible ‘Savannah Sweet’ and ‘Golden eye’ following inoculation with FBR agent. As a complimentary to the related antioxidant enzymes, we also characterized the critical transcription factors (TFs); R1, R5 and RGA29 marker genes using qRT-PCR involved in endowing resistance at three time course/week post inoculation (wpi). Additionally, we analyzed the changes in biomass growth parameters (BGPs) accordingly. The results showed that the lowest disease severity percentage (DSP) was in resistant ‘Saba’ and ‘Saba – HS’ (6.7 and 4.7 %), and the highest ones in susceptible ‘Savannah Sweet’ and ‘Golden eye’ (88.9 and 89.6%), respectively. There was also a significant increase in CAT, POX, and SOD activities in resistant genotypes. The highest CAT activity was recorded in resistant ‘Saba’- 4.91, and Saba-HS - 3.75 fold, followed by the same trend relatively for POX (‘Saba’- 3.53 and ‘Saba-HS’- 2.35 fold); and SOD (‘Saba’ 14.46 -fold and ‘Saba-HS’ 12.95 –fold) significantly. It was also found that FOC could up-regulate the defense marker genes; R1, PR5, and RGA29 expression in resistant '‘Saba’' and ‘Saba’-HS ranging from 1.23 to 5.01-fold. The highest regulation was in PR5-5.01 fold followed by R1-4.78; and RGA29-3.83-fold, respectively. In addition, BGPs displayed almost similar trends as was in enzyme and gene expression accordingly. These findings lead to introduction of newly FBR-resistant onion genotypes, and also can be implicated in integrated pest management planning programs against FBR disease. Cumulatively, the proteomic-transcriptomic-data provide novel-insights for improving onion-breeding programs against FBR disease.

show abstract

Section: Discussionsupporting

confidence: 84%

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Poursakhi,

Asadi-Gharneh,

Esfahani

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…It is of note that, like those identified from any transcriptomic analysis, candidate genes identified in this study also need to be further evaluated. Quantitative polymerase chain reaction (qPCR) has been widely used in validating the expression levels of such genes (Moatamedi et al 2023 ; Qalavand et al 2023 ). As having been reported in many studies, data from qPCR analyses are usually consistent with expression patterns obtained from transcriptome or RNA-seq analyses (Habib et al 2018 ; Gao et al 2019a , 2023 ; Soheili-Moghaddam et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic insights into shared responses to Fusarium crown rot infection and drought stresses in bread wheat (Triticum aestivum L.)

Su,

Gao,

Zheng

et al. 2024

Theor Appl Genet

View full text Add to dashboard Cite

Key message Shared changes in transcriptomes caused by Fusarium crown rot infection and drought stress were investigated based on a single pair of near-isogenic lines developed for a major locus conferring tolerance to both stresses. Abstract Fusarium crown rot (FCR) is a devastating disease in many areas of cereal production worldwide. It is well-known that drought stress enhances FCR severity but possible molecular relationship between these two stresses remains unclear. To investigate their relationships, we generated several pairs of near isogenic lines (NILs) targeting a locus conferring FCR resistance on chromosome 2D in bread wheat. One pair of these NILs showing significant differences between the two isolines for both FCR resistance and drought tolerance was used to investigate transcriptomic changes in responsive to these two stresses. Our results showed that the two isolines likely deployed different strategies in dealing with the stresses, and significant differences in expressed gene networks exist between the two time points of drought stresses evaluated in this study. Nevertheless, results from analysing Gene Ontology terms and transcription factors revealed that similar regulatory frameworks were activated in coping with these two stresses. Based on the position of the targeted locus, changes in expression following FCR infection and drought stresses, and the presence of non-synonymous variants between the two isolines, several candidate genes conferring resistance or tolerance to these two types of stresses were identified. The NILs generated, the large number of DEGs with single-nucleotide polymorphisms detected between the two isolines, and the candidate genes identified would be invaluable in fine mapping and cloning the gene(s) underlying the targeted locus.

show abstract

Identification of genes governing resistance to PCN (Globodera rostochiensis) through transcriptome analysis in Solanum tuberosum

Bairwa

Sood

Bhardwaj

et al. 2023

Funct Integr Genomics

View full text Add to dashboard Cite

Transcriptome-proteomic analysis associated with resistance to wheat cyst nematode Heterodera filipjevi

Cited by 10 publications

References 24 publications

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Transcriptomic insights into shared responses to Fusarium crown rot infection and drought stresses in bread wheat (Triticum aestivum L.)

Identification of genes governing resistance to PCN (Globodera rostochiensis) through transcriptome analysis in Solanum tuberosum

Contact Info

Product

Resources

About