Transcriptome repository of North-Western Himalayan endangered medicinal herbs: a paramount approach illuminating molecular perspective of phytoactive molecules and secondary metabolism

Analysis of Codon Preferences in Medicago ruthenica based on Transcriptome Data

“…Transcriptomics is currently the most direct and universal way to study genome-level changes (Jing et al, 2016), which broadly refers to the study of the abundance of all the RNA transcripts of an organ or tissue under a particular condition. Transcriptome high-throughput sequencing technology has become an important means of studying plant development and elaborating gene mining and expression regulation related to active components and secondary metabolic biosynthesis pathways in herbaceous plants (Kapoor et al, 2021) and in recent years, transcriptome sequencing has begun to be applied in the evaluation of forage grass germplasm resources, the mechanism of formation of important traits, as well as the mining of excellent genetic resources and the development of molecular markers, for example, in Medicago sativa (Zhang et al, 2015), Setaria viridis (Martin et al, 2016), Leymus chinensis (Sun et al, 2013), Elymus nutans (Fu, 2017) and Stipa breviflora (Cao, 2015).…”

Evaluation of Candidate Reference Genes for Gene Expression Analysis in Wild Lamiophlomis rotata

“…With the development of high-throughput sequencing technology, plant RNA-seq datasets can be obtained rapidly and comprehensively [ 24 , 25 ]. This technology is now widely used in transcriptome gene expression analysis [ 26 ], secondary metabolism studies of plants [ 27 , 28 ], disease resistance mechanisms [ 29 ], biocontrol [ 30 , 31 ], and so on. RNA-seq datasets can also be used to study the molecular function of specific genes and to screen for internal reference genes [ 32 , 33 ].…”