Transcriptome sequencing analysis of porcine MDM response to FSL-1 stimulation

Zhou, Lisheng; Wang, Li; Zhao, Weimin; Ren, Shuai; Feng, Teng; Fu, Yabo; Li, Bixia; Wang, Xuemin; Fang, Xiaoyan

doi:10.1016/j.micpath.2019.103830

Cited by 4 publications

(3 citation statements)

References 65 publications

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“…The total RNA was diluted into a concentration of 100 ng/µL and 3 µg were sent for stranded paired-end mRNA-seq sequencing in Centro Nacional de Análisis Genómico (CNAG-CRG, Barcelona, Spain) on a HiSeq2000 sequence analyser (Ilumina, Inc., San Diego, CA, USA). Currently, several RNA-seq experiments are performed at a low replication level and several publications suggest that a minimum of 2–3 replicates can be considered [ 22 , 23 , 24 ].…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds

Albuquerque

Óvilo

Núñez

et al. 2021

Animals

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Gene expression is one of the main factors to influence meat quality by modulating fatty acid metabolism, composition, and deposition rates in muscle tissue. This study aimed to explore the transcriptomics of the Longissimus lumborum muscle in two local pig breeds with distinct genetic background using next-generation sequencing technology and Real-Time qPCR. RNA-seq yielded 49 differentially expressed genes between breeds, 34 overexpressed in the Alentejano (AL) and 15 in the Bísaro (BI) breed. Specific slow type myosin heavy chain components were associated with AL (MYH7) and BI (MYH3) pigs, while an overexpression of MAP3K14 in AL may be associated with their lower loin proportion, induced insulin resistance, and increased inflammatory response via NFkB activation. Overexpression of RUFY1 in AL pigs may explain the higher intramuscular (IMF) content via higher GLUT4 recruitment and consequently higher glucose uptake that can be stored as fat. Several candidate genes for lipid metabolism, excluded in the RNA-seq analysis due to low counts, such as ACLY, ADIPOQ, ELOVL6, LEP and ME1 were identified by qPCR as main gene factors defining the processes that influence meat composition and quality. These results agree with the fatter profile of the AL pig breed and adiponectin resistance can be postulated as responsible for the overexpression of MAP3K14′s coding product NIK, failing to restore insulin sensitivity.

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Section: Methodsmentioning

confidence: 99%

Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds

Albuquerque

Óvilo

Núñez

et al. 2021

Animals

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“…From this study, four randomly selected individuals from AL and BI breeds were chosen for transcriptome analysis by RNA-seq. Currently, many RNA-seq experiments are performed at a low replication level and several publications suggest that a minimum of 2-3 replicates can be considered [44][45][46]. Selected AL pigs averaged a total of 155.0 days on trial with an average daily gain of 571.6 g/d while BI pigs averaged a total of 140.0 days on trial and an average daily gain of 619.4 g/d.…”

Section: Alentejano and Bísaro: Local Pig Breeds With Recognizable Mementioning

confidence: 99%

Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds

Albuquerque

Óvilo

Núñez

et al. 2020

Genes

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When compared to modern lean-type breeds, Portuguese local Alentejano (AL) and Bísaro (BI) pig breeds present a high potential for subcutaneous and intramuscular fat (IMF) deposition which contributes for better meat quality. The aim of this work was to explore the genome function to better understand the underlying physiological mechanisms associated with body fat accretion. Dorsal subcutaneous fat samples were collected at slaughter from adult animals (n = 4 for each breed) with~150 kg body weight. Total RNA was obtained and sequenced for transcriptome analysis using DESeq2. A total of 458 differentially expressed (DE) genes (q-value < 0.05) were identified, with 263 overexpressed in AL and 195 in BI. Key genes involved in de novo fatty acid biosynthesis, elongation and desaturation were upregulated in AL such as ACLY, FASN, ME1, ELOVL6 and SCD. A functional enrichment analysis of the DE genes was performed using Ingenuity Pathway Analysis. Cholesterol synthesis is suggested to be higher in AL via SREBF2, SCAP and PPARG, while lipolytic activity may be more active in BI through GH and AMPK signalling. Increased signalling of CD40 together with the predicted activation of INSIG1 and INSIG2 in BI suggests that this breed is more sensitive to insulin whereas the AL is less sensitive like the Iberian breed.Genes 2020, 11, 422 2 of 20 inherent genetic value for biodiversity, local breeds are used to produce high quality dry-cured meat products, representing an important role in local economies, culture and landscape as the basics of sustainable local pork chains [2,4].Alentejano (AL) and Bísaro (BI) are the two main Portuguese pig breeds. AL evolved from the primitive Sus scrofa mediterraneus, belongs to the Mediterranean group of breeds [5] and is genetically similar to the Iberian (IB) pig [6]. This breed is commonly raised in the south of Portugal and generally characterized by its light bone structure, black color, short and slim extremities and energetic nature [7]. A medium-sized pig, the AL grows at a low rate (except under the finishing phase "montanheira") and presents a low prolificacy [8]. On the other hand, its high and early adipogenic activity provides a meat and fat composition that is attractive for both fresh meat market and for processing high-grade sausage and dry cured products [9,10]. Traditionally, the AL pig is raised under extensive conditions in an integrated pastoral system ("montado") and during the fattening season is fed with acorns from the existing Quercus forests from October to February [5,7]. BI breed belongs to the Celtic group [5], sharing ancestors with highly productive breeds such as Large-White and Landrace. Production of BI is distributed throughout the north of Portugal, from the Tagus River to the northern border with Spain. It is characterized by its docile temperament, presenting a large body with large legs, head and shoulders. BI pig presents a higher prolificacy and productivity than AL, though lower than other genotypes with similar origins but raised under intensive ...

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“…Moreover, during the inflammatory response caused by M. hyopneumoniae infection, the expression of inflammatory factors may also have other regulatory effects on molecular pathways. Studies have reported that many pathways are involved in regulating the expression of inflammatory cytokines mediated by mycoplasmal lipopeptide Pam2CGDPKHPKSF (FSL-1) in pig monocyte-derived macrophages (MDMs); these pathways include the TNF signaling pathway, the cytokine-cytokine receptor interaction pathway, the Toll-like receptor signaling pathway, the janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway, the chemokine signaling pathway, the nucleotide oligomerization domain (NOD)-like receptor signaling pathway, and the NF-κB signaling pathway [35]. Furthermore, previous reports have shown that activated PXR inhibits the activity of NF-κB and the expression of the NF-κB target gene IL-6 [36].…”

Section: Discussionmentioning

confidence: 99%

Effect of Pregnane X Receptor on CYP3A29 Expression in Porcine Alveolar Macrophages during Mycoplasma hyopneumoniae Infection

Yang

Xing

Wang

et al. 2021

Animals

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Mycoplasma hyopneumoniae (M. hyopneumoniae, Mhp) is the causative agent of mycoplasma pneumonia of swine (MPS). M. hyopneumoniae infection causes inflammation in pigs and leads to considerable economic losses in the pig industry. Pregnane X receptor (PXR) is a pluripotent gene regulatory protein that plays an important role in regulating cytochrome P-450 (CYP) in pigs in the context of inflammatory responses, drug metabolism, homeostasis, etc. We previously reported that cytochrome P450 3A29 (CYP3A29) expression was significantly upregulated in pigs infected with M. hyopneumoniae compared with healthy control pigs. This experiment mainly focused on identifying the role of PXR in the regulation of CYP3A29 and inflammatory factors after M. hyopneumoniae infection by establishing pig alveolar macrophage (PAM) cells in which PXR was overexpressed or silenced. Our results showed that the overexpression of PXR could significantly improve the protein and the mRNA expression levels of CYP3A29 with and without M. hyopneumoniae infection in PAM cells. After the expression of PXR was inhibited, protein and mRNA expression levels of CYP3A29 were significantly reduced with and without M. hyopneumoniae infection in PAM cells. Moreover, PXR can regulate the mRNA expression levels of IL-6 and IL-8 during M. hyopneumoniae infection of PAM cells. In conclusion, these results suggest that PXR positively regulates CYP3A29 expression during the inflammatory response caused by M. hyopneumoniae infection.

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Transcriptome sequencing analysis of porcine MDM response to FSL-1 stimulation

Cited by 4 publications

References 65 publications

Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds

Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds

Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds

Effect of Pregnane X Receptor on CYP3A29 Expression in Porcine Alveolar Macrophages during Mycoplasma hyopneumoniae Infection

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