Transcriptome sequencing to unravel the molecular mechanisms underlying the cuticle liquefaction of Antheraea pernyi following Antheraea pernyi nucleopolyhedrovirus challenge

Wang, Guobao; Na, Shuang; Duan, Xiaoxia; Leng, Zheming; Jiang, Yiren; Sheng, Shi; Yang, Ru; Qin, Li

doi:10.1016/j.molimm.2019.03.009

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…Although several PBPs have been identified in A. pernyi (Li et al 2020), there is still a lack of detailed reports on response mechanisms of male moths to female stimulation especially the related proteins and metabolites underlying the female pheromone signal transmission in the male antennae of A. pernyi . It has been investigated the mechanisms of multiple physiological and pathological processes in A. pernyi (Li et al 2016, Wang et al 2019a, 2019b) by HTS technologies. In this study, HTS strategy including LC/MS-based metabolomics and proteomic quantification was applied to explore metabolic and proteomic changes of antennae of male moths displaying wing-flapping (WF males) following stimulation by females compared with the non-stimulated males of A. pernyi .…”

Section: Introductionmentioning

confidence: 99%

Exploring the proteins and metabolites associated with male antennae responses to female exposure of Antheraea pernyi (Lepidoptera: Saturniidae) moths

Wang

2023

Journal of Economic Entomology

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Detection of sex pheromones of insects relies on the antennae. The female pheromone signal transmission in the male antennae ultimately initiates the courtship and mating behaviors of males. To investigate the proteins and metabolites involved in this neural transduction, integrative proteomics and metabolomics analysis including tandem mass tag (TMT) proteomic quantification and liquid chromatography tandem mass spectrometry (LC/MS)-based metabolomics was adopted for comparing proteomic and metabolic changes between the antennae of male moths following stimulation by females and the non-stimulated males of Antheraea pernyi (Guérin-Méneville, Lepidoptera: Saturniidae) in this study. A total of 92 differentially expressed proteins (DEPs) containing 52 upregulated and 40 downregulated proteins and 545 differentially expressed metabolites (DEMs) including 218 upregulated and 327 downregulated metabolites were identified from the antennae of female-stimulated male moths based on the proteome and metabolome data, respectively. Bioinformatics analysis was performed for the 45 DEPs and 160 DEMs, including Gene Ontology (GO), Clusters of Orthologous Groups (COG), and Kyoto Encylopaedia of Genes and Genomes (KEGG) enrichment analysis and Human Metabolome Database (HMDB) annotation. A number of DEPs and DEMs related to neural transmission of female pheromone signals in the male antennae of A. pernyi were screened, including tyrosine hydroxylase, cryptochrome-1, tachykinin, arylalkylamine N-acetyltransferase, cadherin-23, glutathione S-transferase delta 3, tyramine, tryptamine, n-oleoyl dopamine, n-stearoyl dopamine, and n-stearoyl tyrosine. The altered expression levels of those proteins or metabolites were speculated involved in regulating the neuron activity for enhanced transmission of neural impulses and continuous perception, reception, and transduction of female pheromone signals. Our findings yielded novel insights into the potential mechanisms in the antennae of male A. pernyi responding to female attraction.

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Section: Introductionmentioning

confidence: 99%

Exploring the proteins and metabolites associated with male antennae responses to female exposure of Antheraea pernyi (Lepidoptera: Saturniidae) moths

Wang

2023

Journal of Economic Entomology

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“…Previous studies on A. pernyi have primarily focused on cloning and functional analysis of certain structural or stress‐related protein‐coding genes (Liu et al., 2014; Liu, Liu, et al., 2018; Liu, Xin, et al., 2018; Liu, Xin, et al., 2018; Liu, You, et al., 2014; Sezutsu & Yukuhiro, 2000; Yukuhiro, Kanda, & Tamura, 1997; Zhang et al., 2015), along with investigating its role in nucleopolyhedrovirus infection (Liu, Wang, et al., 2019; Qian et al., 2013; Sun et al., 2017; Wang, Na, Duan, et al., 2019; Wang, Na, & Qin, 2019; Zhang, Zhu, Dai, L. Liu, & Luo, 2016), and the nature of the silk fibre (Silva, Kundu, Lu, Reis, & Kundu, 2019; Suzuki, Chirila, & Edwards, 2016; Zhang, Yang, Shao, & Hu, 2010). These studies identified a few key genes, along with providing some insights into the characteristics of viral infection and the properties of natural silk.…”

Section: Introductionmentioning

confidence: 99%

A chromosome‐scale genome assembly of Antheraea pernyi (Saturniidae, Lepidoptera)

Duan

Liu

et al. 2020

Molecular Ecology Resources

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Antheraea pernyi is a semi‐domesticated lepidopteran insect species valuable to the silk industry, human health, and ecological tourism. Owing to its economic influence and developmental properties, it serves as an ideal model for investigating divergence of the Bombycoidea super family. However, studies on the karyotype evolution and functional genomics of A. pernyi are limited by scarce genomic resource. Here, we applied PacBio sequencing and chromosome structure capture technique to assemble the first high‐quality A. pernyi genome from a single male individual. The genome is 720.67 Mb long with 49 chromosomes and a 13.77‐Mb scaffold N50. Approximately 441.75 Mb, accounting for 60.74% of the genome, was identified as repeats. The genome comprises 21,431 protein‐coding genes, 85.22% of which were functionally annotated. Comparative genomics analysis suggested that A. pernyi diverged from its common ancestor with A. yamamai ~30.3 million years ago, and that chromosome fission contributed to the increased chromosome number. The genome assembled in this work will not only facilitate future research on A. pernyi and related species but also help to progress comparative genomics analyses in Lepidoptera.

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Tissue-associated profiling of gene expression in the fifth-instar larvae of Chinese Oak Silkworm, Antheraea pernyi

Duan

Liang

Zeng

et al. 2023

Journal of Asia-Pacific Entomology

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Transcriptome sequencing to unravel the molecular mechanisms underlying the cuticle liquefaction of Antheraea pernyi following Antheraea pernyi nucleopolyhedrovirus challenge

Cited by 6 publications

References 27 publications

Exploring the proteins and metabolites associated with male antennae responses to female exposure of Antheraea pernyi (Lepidoptera: Saturniidae) moths

Exploring the proteins and metabolites associated with male antennae responses to female exposure of Antheraea pernyi (Lepidoptera: Saturniidae) moths

A chromosome‐scale genome assembly of Antheraea pernyi (Saturniidae, Lepidoptera)

Tissue-associated profiling of gene expression in the fifth-instar larvae of Chinese Oak Silkworm, Antheraea pernyi

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