Transcriptomes analysis of Pichia kudriavzevii UniMAP 3–1 in response to acetic acid supplementation in glucose and xylose medium at elevated fermentation temperature

Rahman, Khadijah Hanim Abdul; Najimudin, Nazalan; Ismail, Ku Syahidah Ku

doi:10.1016/j.procbio.2022.03.027

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…Genes for ergosterol biosynthesis are required for tolerance to vanillin stress 99 , while several genes involved in ionic homeostasis, heat protection, trehalose synthesis, antioxidant defense, and ATP production have been shown to be involved in ethanol stress in S. cerevisiae 100 . A recent study by Rahman et al 101 demonstrated that the response of P. kudriavzevii toward acetic acid includes the activation of genes involved in the tricarboxylic acid cycle, ATP-binding cassette transporters, and protein folding, sorting and degradation. Thus, it could be concluded from this information that different organisms employ different mechanisms to deal with different stress conditions.…”

Section: Resultsmentioning

confidence: 99%

Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass

Dolpatcha,

Phong,

Thanonkeo

et al. 2023

Sci Rep

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Second-generation bioethanol production using lignocellulosic biomass as feedstock requires a highly efficient multistress-tolerant yeast. This study aimed to develop a robust yeast strain of P. kudriavzevii via the adaptive laboratory evolution (ALE) technique. The parental strain of P. kudriavzevii was subjected to repetitive long-term cultivation in medium supplemented with a gradually increasing concentration of acetic acid, the major weak acid liberated during the lignocellulosic pretreatment process. Three evolved P. kudriavzevii strains, namely, PkAC-7, PkAC-8, and PkAC-9, obtained in this study exhibited significantly higher resistance toward multiple stressors, including heat, ethanol, osmotic stress, acetic acid, formic acid, furfural, 5-(hydroxymethyl) furfural (5-HMF), and vanillin. The fermentation efficiency of the evolved strains was also improved, yielding a higher ethanol concentration, productivity, and yield than the parental strain, using undetoxified sugarcane bagasse hydrolysate as feedstock. These findings provide evidence that ALE is a practical approach for increasing the multistress tolerance of P. kudriavzevii for stable and efficient second-generation bioethanol production from lignocellulosic biomass.

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Section: Resultsmentioning

confidence: 99%

Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass

Dolpatcha,

Phong,

Thanonkeo

et al. 2023

Sci Rep

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“…In particular, the CZ4 group reached the highest L-serine yield of 428.42 mg/L by 13 h of fermentation. Changes in the composition of the medium may have an impact on a strain’s metabolic pathways, and the status of the synthesis of the target product is subsequently altered [ 28 ]. It can be seen that the carbon–nitrogen ratio of 5 was the most favorable for E. coli to synthesize L-serine, and the consumption capacity of glucose was also stronger than the other experimental groups, while the accumulation of bacterial concentration was lower than the other experimental groups.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis Reveals Potential Mechanisms of L-Serine Production by Escherichia coli Fermentation in Different Carbon–Nitrogen Ratio Medium

Chen

et al. 2022

Foods

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L-serine is an industrially valuable amino acid that is widely used in the food, cosmetics and pharmaceutical industries. In this study, transcriptome sequencing technology was applied to analyze the changes in gene expression levels during the synthesis of L-serine in Escherichia coli fermentation. The optimal carbon–nitrogen ratio for L-serine synthesis in E. coli was determined by setting five carbon–nitrogen ratios for shake flask fermentation. Transcriptome sequencing was performed on E. coli fermented in five carbon–nitrogen ratio medium in which a total of 791 differentially expressed genes (DEGs) were identified in the CZ4_vs_CZ1 group, including 212 upregulated genes and 579 downregulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of these DEGs showed that the effect of an altered carbon–nitrogen ratio on the fermentability of E. coli was mainly focused on metabolic pathways such as GABAergic synapse and the two-component system (TCS) in which the genes playing key roles were mainly gadB, gadA, glsA, glnA, narH and narJ. In summary, these potential key metabolic pathways and key genes were proposed to provide valuable information for improving glucose conversion during E. coli fermentation.

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“…After the uptake of xylose into the cells, xylose is converted into xylitol via xylose reductase and NAD(P)H and then to xylulose via xylitol dehydrogenase and cofactor NAD + before being added to a phosphate group to form xylulose-5-phosphate (X5P), which enters the pentose phosphate pathway (PPP), yielding ethanol [70,71]. A study by Rahman et al [72] demonstrated that P. Kudriazevii UniMAP 3-1 produced ethanol from xylose with a yield of 0.019 g-ethanol/g-xylose at 40 • C, and xylitol was detected. This indicated that P. kudriazevii have enzymes to convert xylose to ethanol (xylose reductase and xylitol dehydrogenase) and the transporter needed to uptake xylose into the yeast cells.…”

Section: Conflicts Of Interestmentioning

confidence: 99%

Exploring the Utilization Potential of Spirogyra sp. Biomass for Ethanol Production: A Study on Saccharification Optimization and High-Temperature Ethanol Fermentation

Vichit,

Salakkam,

Fiala

2023

Processes

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Spirogyra sp. is one of the potential feedstocks for bioethanol production, owing to its high carbohydrate and low lignin contents. However, to date, its use has scarcely been reported, particularly in high-temperature ethanol fermentation. The present study investigated the use of Spirogyra biomass as a bioethanol feedstock by optimizing the conditions for biomass saccharification, followed by ethanol fermentation via thermotolerant yeasts, i.e., Saccahromyces cerevisiae DBKKU Y-53, Kluyveromyces marxianus DBKKU Y-102, and Pichia kudriazevii RZ8-1. The optimization of the algal biomass hydrolysis using response surface methodology (RSM) showed that a maximum total sugar production of 14.75 ± 0.13 g/L was attained using 2.67% (v/v) sulfuric acid, 7.97% (w/v) of biomass loading, and 20 min of hydrolysis time. The fermentation of Spirogyra sp. hydrolysate containing 20 g/L of total sugar at 37 °C showed that S. cerevisiae DBKKU Y-53, K. marxianus DBKKU Y-102, and P. kudriazevii RZ8-1 produced 4.05 ± 0.35 g/L, 4.48 ± 0.13 g/L, and 4.47 ± 0.19 g/L of ethanol, respectively. At 40 °C, lower ethanol production of 1.07 ± 0.47 g/L, 3.93 ± 0.24 g/L, and 3.97 ± 0.19 g/L, respectively, were observed. Nevertheless, P. kudriazevii RZ8-1 exhibited a promising potential for the further development of a high-temperature ethanol fermentation process.

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Transcriptomes analysis of Pichia kudriavzevii UniMAP 3–1 in response to acetic acid supplementation in glucose and xylose medium at elevated fermentation temperature

Cited by 5 publications

References 38 publications

Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass

Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass

Transcriptome Analysis Reveals Potential Mechanisms of L-Serine Production by Escherichia coli Fermentation in Different Carbon–Nitrogen Ratio Medium

Exploring the Utilization Potential of Spirogyra sp. Biomass for Ethanol Production: A Study on Saccharification Optimization and High-Temperature Ethanol Fermentation

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