Transcriptomic Analyses Reveal B-Cell Translocation Gene 2 as a Potential Therapeutic Target in Ovarian Cancer

Abstract

Ovarian cancer is the most common and aggressive type of tumor of the female reproductive system. Two factors account for this detrimental clinical presentation: (i) the lack of early detection methods and (ii) the inherently aggressive nature of this malignancy. Currently, transcriptomic analyses have become important tools to identify new targets in different cancer types. In this study, by measuring expression levels in ovarian cancer samples and stem cell samples, we identified 24 tumor suppressor genes consistently associated with poor prognosis. Combined results further revealed a potential therapeutic candidate, BTG2, which belongs to the antiproliferative gene family. Our results showed that BTG2 expression regulated ovarian cancer cell proliferation via G1/S phase cell cycle arrest by regulating Cyclin D1, CDK4, p-AKT, and p-ERK expression. BTG2 also inhibited cell migration by modulating MMP-2 and MMP-9 expression. Furthermore, xenograft models confirmed a growth inhibitory effect of BTG2 in ovarian cancer in vivo. BTG2 was significantly associated with ovarian cancer FIGO stage and grade in the clinic. Our findings indicated that BTG2 exerts a suppressive impact on ovarian cancer and could be a potential biomarker.

[Retracted] Effect of FLOT2 Gene Expression on Invasion and Metastasis of Colorectal Cancer and Its Molecular Mechanism under Nanotechnology and RNA Interference

“…Type IV gelatinase MMP-2 and MMP-9 are the most important MMPs [ 31 ]. Many studies have reported the role of MMP-2 and MMP-9 in the process of EMT [ 32 , 33 ]. The results of this study showed that there was no statistical difference in mRNA level and protein expression of MMP9 gene in the NC group, CK group, and FLOT2-siRNA group ( P > 0.05), and the mRNA level and protein level of MMP2 in the FLOT2-siRNA group were significantly lower than those in the NC group and CK group ( P < 0.01).…”

To identify biomarkers associated with the transfer of diabetes combined with cancer in human genes using bioinformatics analysis

“…[ 42 – 44 ] BTG2 is linked to immune cell infiltration, has an inhibitory effect on ovarian cancer, and can be used as a biomarker in patients with lung adenocarcinoma. [ 45 , 46 ] A recent study found a link between ZFP36 and postoperative osteosarcoma, implying that it could be a potential prognostic biomarker as well as a new therapeutic target for osteosarcoma. [ 47 ]…”

“…[42][43][44] BTG2 is linked to immune cell infiltration, has an inhibitory effect on ovarian cancer, and can be used as a biomarker in patients with lung adenocarcinoma. [45,46] A recent study found a link between ZFP36 and postoperative osteosarcoma, implying that it could be a potential prognostic biomarker as well as a new therapeutic target for osteosarcoma. [47] Although we identified 10 hub genes in diabetic patient samples, the direct participation of these genes in the progression of diabetes has not been established.…”

Construction Strategy of Functionalized Liposomes and Multidimensional Application

“…Take PEG for example, PEG coating on the surface of liposomes can increase the flexibility and hydrophilicity of liposomes, reducing the interaction between liposome lipid membrane and plasma protein, as well as prolonging the circulation time. [129] Aside from PEG, the commonly used modifiers of long-circulating liposomes include chitosan, its lipid derivatives, Tween 80, sodium cholate, Span 60, etc. [130] Among them, chitosan has excellent and significant physical, chemical and biological properties, namely antibacterial activity, biocompatibility, biodegradability, low toxicity, blood compatibility and mucosal adhesion.…”