Transcriptomic analysis and biological evaluation reveals that LMO3 regulates the osteogenic differentiation of human adipose derived stem cells via PI3K/Akt signaling pathway

Kang, Yue; Pei, Wenye

doi:10.1007/s10735-021-10047-5

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…This axis is not only related to phosphatidylinositol but also a signal pathway derived through RTK mediation [ 45 ]. Kang et al demonstrated through transcriptomic analysis and biological evaluation that the PI3K/AKT signaling pathway regulates the osteogenic differentiation of human stem cells [ 46 ]. In addition, Li et al showed that the m 6 A-modifying enzyme ALKBH5 affected the osteogenic differentiation process of mesenchymal stem cells by regulating PI3K/AKT [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

FOXO1 regulates osteogenic differentiation of periodontal ligament stem cells through the METTL3 signaling pathway

Wang,

Shi,

Lin

et al. 2023

J Orthop Surg Res

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Background Periodontitis is a chronic inflammation that occurs in periodontal tissue and has a high incidence rate. Periodontal ligament stem cells (PDLSCs) are ideal candidates for periodontal tissue and bone regeneration in patients with periodontitis. The purpose of this work was to analyze the molecular mechanisms that affect the osteogenic differentiation of PDLSCs. Methods In this work, qRT‒PCR was used to detect the mRNA expression level of FOXO1 in clinical tissues and PDLSCs. Alkaline phosphatase (ALP) staining and Alizarin red S (ARS) staining were used to detect the degree of osteogenic differentiation of PDLSCs. qRT‒PCR and western blotting were used to measure the levels of the early osteogenic markers COL1A1 and RUNX2. The JASPAR online database was used to predict FOXO1-regulated genes. Results FOXO1 was generally expressed at low levels in clinical samples from patients with periodontitis. We provided evidence that overexpression of FOXO1 promoted osteogenic differentiation in PDLSCs. In addition, both in vitro and rescue experiments showed that FOXO1 regulated METTL3. FOXO1 affected osteogenic differentiation mainly by regulating METTL3 modification of the PI3K/AKT pathway. Conclusions FOXO1 activated the PI3K/AKT signaling pathway by transcriptionally activating METTL3. This effect promoted the osteogenic differentiation of PDLSCs.

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Section: Discussionmentioning

confidence: 99%

FOXO1 regulates osteogenic differentiation of periodontal ligament stem cells through the METTL3 signaling pathway

Wang,

Shi,

Lin

et al. 2023

J Orthop Surg Res

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“… Wagner et al (2021) reported that LMO3 promoted the development of human adipose tissue by modulating the transcriptional activity of PPARγ, which is a key adipogenic master switch. Moreover, LMO3 overexpression enhanced human adipose-derived stem cell osteogenesis through PI3K/Akt signaling ( Kang and Pei, 2022 ). Recently, LMO proteins have been emerging as key molecules in a wide variety of human cancers.…”

Section: Introductionmentioning

confidence: 99%

LMO3 downregulation in PCa: A prospective biomarker associated with immune infiltration

Sun

Wang

et al. 2022

Front. Genet.

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Prostate cancer is the third leading cause of new cancer cases and the second most common tumor type in men globally. LMO3 has been stated to play a vital role in some cancers; however, the prognostic value of LMO3 in PCa remains vague. Here, we utilized various web databases to elucidate in detail the prognostic value and molecular functions of LMO3 in PCa. LMO3 expression was significantly decreased in PCa. Low LMO3 expression was associated with gender, age, and TNM grade and predicted a poor prognosis in PCa patients. Functional enrichment analysis suggested that LMO3 is engaged in the extracellular matrix and immune response. Moreover, LMO3 was positively correlated with immune infiltration levels and numerous immune markers. LMO3 may function as a prospective biomarker of immune infiltration in PCa.

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Transcriptomic analysis and biological evaluation reveals that LMO3 regulates the osteogenic differentiation of human adipose derived stem cells via PI3K/Akt signaling pathway

Cited by 2 publications

References 40 publications

FOXO1 regulates osteogenic differentiation of periodontal ligament stem cells through the METTL3 signaling pathway

FOXO1 regulates osteogenic differentiation of periodontal ligament stem cells through the METTL3 signaling pathway

LMO3 downregulation in PCa: A prospective biomarker associated with immune infiltration

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