Transcriptomic Analysis of Aedes aegypti Innate Immune System in Response to Ingestion of Chikungunya Virus

Zhao, Liming; Alto, Barry W.; Jiang, Yu; Yu, Fahong; Zhang, Yanping

doi:10.3390/ijms20133133

Cited by 19 publications

(22 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Distinct mosquito gene expression profiles underpinned infection response at different ambient temperatures, both in the absolute number of DEGs but also their gene identities. Similar to our findings for 28°C, a recent study of mosquitoes held at 30°C found a larger number of DEGs expressed at 3 dpi in response to CHIKV [ 35 ]. However, exposure to 32°C in our study elicited a surprisingly low number of genes being expressed in response to CHIKV compared with control uninfected mosquitoes, particularly at the earlier time point.…”

Section: Discussionsupporting

confidence: 91%

“…Using a literature review, we identified 10 additional gene families, resulting in a total of 37 gene families implicated in mosquito immunity (electronic supplementary material, table S4), producing a final list of 998 genes. The list was divided into classical or non-classical components (electronic supplementary material, tables S5 and S6) [ 26 – 35 ]. Genes already identified and directly involved in humoral and cellular immune response were considered as classical immune genes [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Temperature modulates immune gene expression in mosquitoes during arbovirus infection

et al. 2021

View full text Add to dashboard Cite

The principal vector of dengue, Zika and chikungunya viruses is the mosquito Aedes aegypti , with its ability to transmit pathogens influenced by ambient temperature. We use chikungunya virus (CHIKV) to understand how the mosquito transcriptome responds to arbovirus infection at different ambient temperatures. We exposed CHIKV-infected mosquitoes to 18, 28 and 32°C, and found that higher temperature correlated with higher virus levels, particularly at 3 days post infection, but lower temperature resulted in reduced virus levels. RNAseq analysis indicated significantly altered gene expression levels in CHIKV infection. The highest number of significantly differentially expressed genes was observed at 28°C, with a more muted effect at the other temperatures. At the higher temperature, the expression of many classical immune genes, including Dicer-2 , was not substantially altered in response to CHIKV. The upregulation of Toll, IMD and JAK-STAT pathways was only observed at 28°C. Functional annotations suggested that genes in immune response and metabolic pathways related to energy supply and DNA replication were involved in temperature-dependent changes. Time post infection also led to substantially different gene expression profiles, and this varied with temperature. In conclusion, temperature significantly modulates mosquito gene expression in response to infection, potentially leading to impairment of immune defences at higher temperatures.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Temperature modulates immune gene expression in mosquitoes during arbovirus infection

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Distinct mosquito gene expression profiles underpinned infection response at different ambient temperatures, both in the absolute number of DEGs but also their gene identities. Similar to our findings for 28 °C, a recent study of mosquitoes held at 30 °C found a larger number of DEGs expressed at 3 dpi in response to CHIKV [37]. However, exposure to 32 °C in our study elicited a surprisingly low number of genes being expressed in response to CHIKV compared to control uninfected mosquitoes, particularly at the earlier time point.…”

Section: Discussionsupporting

confidence: 88%

“…The first step in eliciting an immune response is the recognition of pathogens by pattern recognition receptors [40]. Similar to previous studies [37–39, 41], we identified diverse PRR genes being differentially expressed in our study, including CLIP-domain serine protease family B, FREP, LRR, leucine-rich transmembrane proteins, CTLs, TEP and Galectins. However, we did not find any PRR DEGs common across all temperature treatments at 3 dpi, while at 7 dpi only CLIPD6 was shared.…”

Section: Discussionsupporting

confidence: 82%

Temperature alters gene expression in mosquitoes during arbovirus infection

Wimalasiri-Yapa

Barrero

Stassen

et al. 2020

Preprint

View full text Add to dashboard Cite

Arthropod-borne viruses (arboviruses) such as dengue, Zika and chikungunya constitute a significant proportion of the global disease burden. The principal vector of these pathogens is the mosquito Aedes (Ae.) aegypti, and its ability to transmit virus to a human host is influenced by environmental factors such as temperature. However, exactly how ambient temperature influences virus replication within mosquitoes remains poorly elucidated, particularly at the molecular level. Here, we use chikungunya virus (CHIKV) as a model to understand how the host mosquito transcriptome responds to arbovirus infection under different ambient temperatures. We exposed CHIKV-infected mosquitoes to 18 °C, 28 °C and 32 °C, and found higher temperature correlated with higher virus replication levels, particularly at early time points post-infection. Lower ambient temperatures resulted in reduced virus replication levels. Using RNAseq, we found that temperature significantly altered gene expression levels in mosquitoes, particularly components of the immune response. The highest number of significantly differentially expressed genes in response to CHIKV was observed at 28 °C, with a markedly more muted effect observed at either lower (18 °C) or higher (32 °C) temperatures. At the higher temperature, the expression of many classical immune genes, including Dicer-2 in the RNAi pathway, was not substantially altered in response to CHIKV. Upregulation of Toll, IMD and JAK-STAT pathways was only observed at 28 °C. Time post infection also led to substantially different gene expression profiles, and this effect varied depending upon the which temperature mosquitoes were exposed to. Taken together, our data indicate temperature significantly modulates mosquito gene expression in response to infection, potentially leading to impairment of immune defences at higher ambient temperatures.

show abstract

“…In total, we found 18 unique genes, representing the predicted targets for 12 out of the 14 miRNAs from our study, which were shown to be significantly modulated in different studies involving whole Ae. aeygpti , midgut and carcass with CHIKV, DENV, WNV, or ZIKV at 1 dpi, 2 dpi, 4 dpi, 7 dpi, or 14 dpi [ 102 , 103 , 104 , 105 ]. We focused on significantly downregulated transcripts that were predicted as targets for the significantly upregulated miRNAs in this study.…”

Section: Resultsmentioning

confidence: 99%

Ross River Virus Provokes Differentially Expressed MicroRNA and RNA Interference Responses in Aedes aegypti Mosquitoes

Sinclair

Asgari

2020

Viruses

View full text Add to dashboard Cite

Alphaviruses are globally distributed and predominately transmitted by mosquitoes. Aedes species are common vectors for the clinically important alphaviruses—Chikungunya, Sindbis, and Ross River (RRV) viruses—with Aedes aegypti also being a vector for the flaviviruses dengue, Yellow Fever, and Zika viruses. Ae. aegypti was putatively implicated in the large 1979–1980 South Pacific Islands outbreak of RRV—the leading cause of arboviral disease in Australia today. The RNA interference (RNAi) defense response in mosquitoes involves a number of small RNAs, with their kinetics induced by alphaviruses being poorly understood, particularly at the tissue level. We compared the small RNA profiles between RRV-infected and non-infected Ae. aegypti midgut and fat body tissues at 2, 6, and 12 days post-inoculation (dpi). RRV induced an incremental RNAi response, yielding short interfering and P-element-induced-wimpy-testis (PIWI)-interacting RNAs. Fourteen host microRNAs were differentially expressed due to RRV with the majority in the fat body at 2 dpi. The largely congruent pattern of microRNA regulation with previous reports for alphaviruses and divergence from those for flaviviruses suggests a degree of conservation, whereas patterns of microRNA expression unique to this study provide novel insights into the tissue-specific host-virus attributes of Ae. aegypti responses to this previously unexplored old-world alphavirus.

show abstract

Transcriptomic Analysis of Aedes aegypti Innate Immune System in Response to Ingestion of Chikungunya Virus

Cited by 19 publications

References 60 publications

Temperature modulates immune gene expression in mosquitoes during arbovirus infection

Temperature modulates immune gene expression in mosquitoes during arbovirus infection

Temperature alters gene expression in mosquitoes during arbovirus infection

Ross River Virus Provokes Differentially Expressed MicroRNA and RNA Interference Responses in Aedes aegypti Mosquitoes

Contact Info

Product

Resources

About