Transcriptomic analysis of effects of 1-methylcyclopropene (1-MCP) and ethylene treatment on kiwifruit (Actinidia chinensis) ripening

Abstract

Ethylene (ET) is a gaseous phytohormone with a crucial role in the ripening of many fruits, including kiwifruit (Actinidia spp.). Meanwhile, treatment with 1-methylcyclopropene (1-MCP), an artificial ET inhibitor delays the ripening of kiwifruit. The objective of this study was to determine the effect of ET and 1-MCP application during time-course storage of kiwifruit. In addition, we aimed to elucidate the molecular details underlying ET-mediated ripening process in kiwifruit. For this purpose, we conducted a time-course transcriptomic analysis to determine target genes of the ET-mediated maturation process in kiwifruit during storage. Thousands of genes were identified to be dynamically changed during storage and clustered into 20 groups based on the similarity of their expression patterns. Gene ontology analysis using the list of differentially expressed genes (DEGs) in 1-MCP-treated kiwifruit revealed that the identified DEGs were significantly enriched in the processes of photosynthesis metabolism and cell wall composition throughout the ripening process. Meanwhile, ET treatment rapidly triggered secondary metabolisms related to the ripening process, phenylpropanoid (e.g. lignin) metabolism, and the biosynthesis of amino acids (e.g. Phe, Cys) in kiwifruit. It was demonstrated that ET biosynthesis and signaling genes were oppositely affected by ET and 1-MCP treatment during ripening. Furthermore, we identified a ET transcription factor, AcEIL (Acc32482) which is oppositely responsive by ET and 1-MCP treatment during early ripening, potentially one of key signaling factor of ET- or 1-MCP-mediated physiological changes. Therefore, this transcriptomic study unveiled the molecular targets of ET and its antagonist, 1-MCP, in kiwifruit during ripening. Our results provide a useful foundation for understanding the molecular details underlying the ripening process in kiwifruit.

Agrobacterium rhizogenes-mediated marker-free transformation and gene editing system revealed that AeCBL3 mediates the formation of calcium oxalate crystal in kiwifruit

“…deliciosa, A. eriantha, and A. arguta) have been developed as commercial cultivars so far. Due to its nutrient and commercial values, extensive investigations on genomics (Huang et al 2013;Pilkington et al 2018;Tang et al 2019;Wu et al 2019;Yue et al 2023), transcriptomics (Choi et al 2022;Tahir et al 2022;Miao et al 2023;Xiong et al 2023), and metabolomics (Abid et al 2022;Jia et al 2022;Wang et al 2022a;Wang et al 2022c;Shu et al 2023;Zhang et al 2023) of kiwifruit have been carried out. However, functional genomic studies (Akagi et al 2019;Peng et al 2019;Fu et al 2021;Varkonyi-Gasic et al 2021;Wang et al 2022) are limited, partially because the genetic modification of kiwifruit is difficult and time-consuming.…”

Upaya Mempercepat Kematangan Buah Kopi Arabika dengan Aplikasi Hormon

“…Penelitian Choi et al (2023) menunjukkan hal yang sama, yakni etilen mempercepat pematangan buah kiwi dengan memicu metabolisme sekunder yang berkaitan dengan proses pematangan, metabolisme fenilpropanoid (misalnya lignin), dan biosintesis asam amino.…”