Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo

Asfor, Amin S.; Nazki, Salik; Reddy, Vishwanatha R. A. P.; Campbell, Elle A.; Dulwich, Katherine L; Giotis, Efstathios S.; Skinner, Michael A.; Broadbent, Andrew J.

doi:10.3390/v13050933

Cited by 7 publications

(5 citation statements)

References 35 publications

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“…In addition, transcriptomic analysis revealed that more severe disease in line W chicken was associated with significant up-regulation of pathways involved in inflammation, cytoskeletal regulation by Rho GTPases, and Wnt signaling in the bursa, etc. [ 13 ] And another research focused on cecum of 1-day-old chickens infected with SE by RNA-seq, and 104 differentially expressed genes were identified, included IL-22 , IL-1β , etc. inflammation-related genes [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum

Miao

Liu

et al. 2022

BMC Vet Res

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Background Salmonella enterica, serovar Enteritidis (SE) is a food-borne pathogen, which can cause great threat to human health through consumption of the contaminated poultry products. Chicken is the main host of SE. The mRNA and microRNA (miRNA) expression profiles were analyzed on cecum of Shouguang chicken via next-generation sequencing and bioinformatics approaches. The treated group was inoculated SE, and the control group was inoculated with phosphate buffer saline (PBS). Results There were 1760 differentially expressed mRNAs in the SE-infected group, of which 1046 were up-regulated mRNA, and 714 were down-regulated mRNA. In addition, a total of 821 miRNAs were identified, and 174 miRNAs were differentially expressed, of which 100 were up-regulated and 74 were down-regulated. Functional enrichment of differentially expressed mRNAs was similar to miRNA target genes. The functional analysis results of differentially expressed mRNAs and miRNAs were performed. Immune-related processes and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways were enriched by up-regulated mRNA. The down-regulated mRNAs were enriched in tissue development and metabolic-related KEGG pathways. The functional analysis of up-regulated miRNA target genes was similar to the down-regulated mRNAs. The down-regulated miRNA target genes were enriched in metabolic-related GO (Gene Ontology) -BP (Biological process) terms and KEGG pathways. The overlap of the up-regulated mRNA and the up-regulated miRNA target genes (class I) was 325, and the overlap of the down-regulated miRNA target genes (class II) was 169. The class I enriched in the immune-related GO-BP terms and KEGG pathways. The class II mainly enriched in metabolic-related GO-BP terms and KEGG pathways. Then we detected the expression of mRNA and miRNA through qRT-PCR. The results shown that the expression of HHIP, PGM1, HTR2B, ITGB5, RELN, SFRP1, TCF7L2, SCNN1A, NEK7, miR-20b-5p, miR-1662, miR-15a, miR-16-1-3p was significantly different between two groups. Dual-luciferase reporter assay was used to detect the relationship between miR-20b-5p and SCNN1A. The result indicated that miR-20b-5p regulate immune or metabolic responses after SE infection in Shouguang chickens by directly targeting SCNN1A. Conclusions The findings here contribute to the further analysis of the mechanism of mRNA and miRNA defense against SE infection, and provide a theoretical foundation for the molecular disease-resistant breeding of chickens.

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Section: Introductionmentioning

confidence: 99%

Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum

Miao

Liu

et al. 2022

BMC Vet Res

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“…To date, IBD is still one of the most economically important immunosuppressive disease affecting young chickens aged 3–6 weeks old. Previous investigations into the virus’s pathogenesis have predominantly focused on host-pathogen interaction using Omics studies, both in vitro and in vivo , primarily involving host cell type such as fibroblast, DF-1 cells and bursal cells ( 16 , 19 – 21 , 47 , 48 ). However, the thymus, serving as the central immune organ responsible for T cell maturation and differentiation, has been confirmed as an important target tissue in IBDV pathogenesis ( 6 , 11 ).…”

Section: Discussionmentioning

confidence: 99%

“…These findings collectively underscore the important role of thymic pathogenicity in contributing to the immunosuppression/pathology caused by IBDV in the infected chickens. While the BF has been confirmed to be one of the most important target organ for IBDV, numerous researches have focused on deciphering the pathogenicity mechanisms of IBDV by examining changes in the BF or in vitro cultured immune cells (15)(16)(17)(18)(19)(20)(21)(22)(23). However, there are no reports on the molecular pathogenesis of IBDV in the thymus currently.…”

Section: Introductionmentioning

confidence: 99%

RNA-seq reveals role of cell-cycle regulating genes in the pathogenicity of a field very virulent infectious bursal disease virus

Chen,

Wang,

et al. 2024

Front. Vet. Sci.

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Infectious bursal disease virus (IBDV) infection causes highly contagious and immunosuppressive disease in poultry. The thymus, serving as the primary organ for T cell maturation and differentiation, plays an important role in the pathogenicity of IBDV in the infected chickens. However, there are no reports on the molecular pathogenesis of IBDV in the thymus currently. The aim of the study was to elucidate the molecular mechanisms underlying the pathogenicity of a field very virulent (vv) IBDV strain NN1172 in the thymus of SPF chickens using integrative transcriptomic and proteomic analyses. Our results showed that a total of 4,972 Differentially expressed genes (DEGs) in the thymus of NN1172-infected chickens by transcriptomic analysis, with 2,796 up-regulated and 2,176 down-regulated. Meanwhile, the proteomic analysis identified 726 differentially expressed proteins (DEPs) in the infected thymus, with 289 up-regulated and 437 down-regulated. Overall, a total of 359 genes exhibited differentially expression at both mRNA and protein levels, with 134 consistently up-regulated and 198 genes consistently down-regulated, as confirmed through a comparison of the RNA-seq and the proteomic datasets. The gene ontology (GO) analysis unveiled the involvement of both DEGs and DEPs in diverse categories encompassing cellular components, biological processes, and molecular functions in the pathological changes in IBDV-infected thymus. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the host mainly displayed severely disruption of cell survival/repair, proliferation and metabolism pathway, meanwhile, the infection triggers antiviral immune activation with a potential emphasis on the MDA5 pathway. Network inference analysis identified seven core hub genes, which include CDK1, TYMS, MCM5, KIF11, CCNB2, MAD2L1, and MCM4. These genes are all associated with cell-cycle regulating pathway and are likely key mediators in the pathogenesis induced by NN1172 infection in the thymus. This study discovered dominant pathways and genes which enhanced our understanding of the molecular mechanisms underlying IBDV pathogenesis in the thymus.

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“…A broad search of the literature was completed in order to classify lines as either susceptible or resistant to each virus, as much as was possible. Studies of IBV (Bumstead et al, 1989; Cook et al, 1990; Nakamura et al, 1991; Otsuki et al, 1990), IBDV (Asfor et al, 2021; Bumstead et al, 1993; Farhanah et al, 2018; Smith et al, 2015b), MDV (Burgess et al, 2001; Cole, 1968; Mohd Isa et al, 2020) and AIV (Ruiz‐Hernandez et al, 2016) infections were referenced.…”

Section: Methodsmentioning

confidence: 99%

Genetic variation in chicken interferon signalling pathway genes in research lines showing differential viral resistance

et al. 2022

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Avian viruses of economic interest are a significant burden on the poultry industry, affecting production traits and resulting in mortality. Furthermore, the zoonosis of avian viruses risks pandemics developing in humans.Vaccination is the most common method of controlling viruses; however current vaccines often lack cross-protection against multiple strains of each virus. The mutagenicity of these viruses has also led to virulent strains emerging that can overcome the protection offered by vaccines. Breeding chickens with a more robust innate immune response may help in tackling current and emerging viruses. Understanding the genetic evolution of different lines will thus provide a useful tool in helping the host in the fight against pathogens. This study focuses on the interferon genes and their receptors in different chicken lines that are known to be more resistant or susceptible to particular avian viruses. Comparing genotypic differences in these core immune genes between the chicken lines may explain the phenotypic differences observed and aid the identification of causative variations. The relative resistance/susceptibility of each line to viruses of interest (Marek's disease virus, infectious bursal disease, infectious bronchitis virus and avian influenza virus) has previously been determined. Here we identify single nucleotide polymorphisms in interferons and downstream genes. Functional prediction tools were used to identify variants that may be affecting protein structure, mRNA secondary structure or transcription factor and micro-RNA binding sites. These variants were then considered in the context of the research lines and their distribution between phenotypes. We highlight 60 variants of interest in the interferon pathway genes that may account for susceptibility/resistance to viral pathogens.

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Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo

Cited by 7 publications

References 35 publications

Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum

Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum

RNA-seq reveals role of cell-cycle regulating genes in the pathogenicity of a field very virulent infectious bursal disease virus

Genetic variation in chicken interferon signalling pathway genes in research lines showing differential viral resistance

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