Transcriptomic analysis of stress response to novel antimicrobial coatings in a clinical MRSA strain

Vaishampayan, Ankita; Ahmed, Rameez; Wagner, Olaf; Jong, Anne de; Haag, Rainer; Grohmann, Elisabeth

doi:10.1016/j.msec.2020.111578

Cited by 9 publications

(12 citation statements)

References 60 publications

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“…Recently, metal-based nanoenzymes, such as the oxidase-like silver-palladium bimetallic alloy nanocage AgPd 0.38 were shown to produce ROS at the surface and selectively kill drug-resistant bacteria, but did not show toxicity in mammalian cells ( Gao et al, 2021 ). In addition, very promising metal-based antimicrobial surface coatings were developed, including AGXX® and the combination of functionalized graphene oxide (GOX) with AGXX®, termed as GOX-AGXX® ( Landau, 2013 ; Guridi et al, 2015 ; Vaishampayan et al, 2021 ). AGXX® is composed of Ag + and ruthenium (Ru + ), which form an electric field via two redox cycles, leading to electron transfer to molecular oxygen and ROS generation ( Figure 1 ; Guridi et al, 2015 ; Clauss-Lendzian et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The novel combined GOX-AGXX® coating acts via a “catch and kill” mechanism to facilitate bacterial killing ( Vaishampayan et al, 2021 ). GOXs are oxidized graphite sheets, which are grafted with polycationic polymer chains and bind the negatively-charged cell envelope of bacteria ( Vaishampayan et al, 2021 ). Thus, GOX captures bacteria via electrostatic attractions, leading to inhibition of bacterial proliferation as bacteriostatic effect ( Vaishampayan et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…GOXs are oxidized graphite sheets, which are grafted with polycationic polymer chains and bind the negatively-charged cell envelope of bacteria ( Vaishampayan et al, 2021 ). Thus, GOX captures bacteria via electrostatic attractions, leading to inhibition of bacterial proliferation as bacteriostatic effect ( Vaishampayan et al, 2021 ). The second material AGXX® kills the GOX-captured bacteria via the described ROS formation.…”

Section: Introductionmentioning

confidence: 99%

“…AGXX® can be electroplated on various materials, including plastics, steel, glass, ceramics, fleeze, and cellulose fibers. AGXX® coatings are used for sterilization of medical implants, catheters, and plasters as well as in industrial water pipelines to successfully eradicate bacteria, which are in close contact with the AGXX® surface by a process termed as “contact killing” ( Maillard and Hartemann, 2013 ; Guridi et al, 2015 ; Villapún et al, 2016 ; Clauss-Lendzian et al, 2018 ; Vaishampayan et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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The Antimicrobial Activity of the AGXX® Surface Coating Requires a Small Particle Size to Efficiently Kill Staphylococcus aureus

Linzner

Antelmann

2021

Front. Microbiol.

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Methicillin-resistant Staphylococcus aureus (MRSA) isolates are often resistant to multiple antibiotics and pose a major health burden due to limited treatment options. The novel AGXX® surface coating exerts strong antimicrobial activity and successfully kills multi-resistant pathogens, including MRSA. The mode of action of AGXX® particles involves the generation of reactive oxygen species (ROS), which induce an oxidative and metal stress response, increased protein thiol-oxidations, protein aggregations, and an oxidized bacillithiol (BSH) redox state in S. aureus. In this work, we report that the AGXX® particle size determines the effective dose and time-course of S. aureus USA300JE2 killing. We found that the two charges AGXX®373 and AGXX®383 differ strongly in their effective concentrations and times required for microbial killing. While 20–40 μg/ml AGXX®373 of the smaller particle size of 1.5–2.5 μm resulted in >99.9% killing after 2 h, much higher amounts of 60–80 μg/ml AGXX®383 of the larger particle size of >3.2 μm led to a >99% killing of S. aureus USA300JE2 within 3 h. Smaller AGXX® particles have a higher surface/volume ratio and therefore higher antimicrobial activity to kill at lower concentrations in a shorter time period compared to the larger particles. Thus, in future preparations of AGXX® particles, the size of the particles should be kept at a minimum for maximal antimicrobial activity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Antimicrobial Activity of the AGXX® Surface Coating Requires a Small Particle Size to Efficiently Kill Staphylococcus aureus

Linzner

Antelmann

2021

Front. Microbiol.

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Graphene‐Based Bacterial Filtration via Electrostatic Adsorption

Ahmed

Vaishampayan

Achazi

et al. 2022

Adv Materials Inter

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ditions lack clean water, fecal-oral diseases can proliferate rapidly. Diarrhea may not seem deadly to those who have access to improved sanitation, but it kills 750 000 children every year that is more than malaria, AIDS, and measles combined. [2] Waterborne pathogens are also of great concern in the hospital environment, as the water temperatures and the complex structure of hospital water systems are suitable for bacterial growth and biofilm formation. [3,4] These pathogens in connected devices (such as sinks, showers, ice machines, water baths, eyewash stations, and dental units) can lead to severe infections, especially with the rising number of multidrug-resistant bacteria. [5,6] The faucet micropore filters that are used to prevent this outcome must be replaced after a few weeks of use, making them very costly. Novel antibacterial technologies may offer practical and cost-effective prevention strategies for these concerns.Graphene materials have been reported to exhibit antibacterial activity by physically interacting with bacterial cells. [7][8][9] These flexible, singleatom-thick, nano-micrometer sized sheets feature an extremely large surface area. [10,11] Among the variety of different graphene materials, graphene oxide (GO) is frequently used due to its inexpensive preparation from graphite [12][13][14][15][16] as well as its hydrophilic functional groups, which enhance its dispersibility in polar solvents and offer multiple options for chemical post-modification. [17,18] In a previous work we demonstrated that polymer post-modification of micrometer-sized GO can be used to create polycationic microsheets. [19] These flexible GO microsheets matched the size and surface-charge density of opposite charged E. coli bacterial cells. Incubating them with gram-positive methicillinresistant Staphylococcus aureus (MRSA) and gram-negative Escherichia coli (E. coli) led to wrapping and immobilization of bacterial cells in both cases. [19] Based on multivalent electrostatic attraction, these GO derivates (GOX) also showed antibacterial activity when they were attached to a carrier material. [20,21] Although many polycationic polymers like chitosan, [22,23] polyethylene imine, [24,25] ε-polylysine, [26] polysiloxanes, [27,28] polyionenes [29,30] as well as modified GO materials with various conjugated moieties like mannose, [31] lactose, [32] quaternary ammonium compounds [33,34] and zwitterionic systems [35] have been shown to bind and inhibit bacteria, investigation Flexible graphene oxide (GO) microsheets with attached positively charged polymers, termed GOX microsheets, are efficient at bacterial adsorption, as they bind electrostatically to bacterial membranes' negative surface charge. The authors explore an antimicrobial water filter application for GOX's extremely high surface area and its previously described efficient bacterial adsorption. Cellulose-fiber carrier material is functionalized with GOX microsheets to create an adsorption-based bacteria filtration material. The morphology and charge density (7...

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Multi-target anti-MRSA mechanism and antibiotic synergistic effect of marine alkaloid Ascomylactam A in vitro and in vivo against clinical MRSA strains

Wu,

Yao,

Ren

et al. 2025

Biochemical Pharmacology

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Transcriptomic analysis of stress response to novel antimicrobial coatings in a clinical MRSA strain

Abstract: Transcriptomic analysis of stress response to novel antimicrobial coatings in a clinical MRSA strain. Materials science & engineering c-Biomimetic and supramolecular systems, 119, [111578].

Cited by 9 publications

References 60 publications

The Antimicrobial Activity of the AGXX® Surface Coating Requires a Small Particle Size to Efficiently Kill Staphylococcus aureus

The Antimicrobial Activity of the AGXX® Surface Coating Requires a Small Particle Size to Efficiently Kill Staphylococcus aureus

Graphene‐Based Bacterial Filtration via Electrostatic Adsorption

Multi-target anti-MRSA mechanism and antibiotic synergistic effect of marine alkaloid Ascomylactam A in vitro and in vivo against clinical MRSA strains

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