Transcriptomic analysis of the testicular fusion in Spodoptera litura

Chang, Yaqing; Ou, Jun; Liu, Yucheng; Wu, Qiong; Wen, Liang; Zheng, Sichun; Sheng, Li; Feng, Qili; Liu, Lin

doi:10.1186/s12864-020-6494-3

Cited by 10 publications

(12 citation statements)

References 57 publications

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“…The quality assessment was listed in the Supplementary Tables 3 , 4 . The Fragments Per Kilobase of transcript per Million fragments mapped (FPKM) values represent the expression levels of transcripts ( Chen et al, 2020 ). Analysis of differentially expressed transcripts (DETs) was performed by using FPKM value of the log2(fold changes) >1 and the standard of false discovery rate (FDR) <0.001.…”

Section: Methodsmentioning

confidence: 99%

Gut Microbiota Dysbiosis Influences Metabolic Homeostasis in Spodoptera frugiperda

et al. 2021

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Insect gut microbiota plays important roles in acquiring nutrition, preventing pathogens infection, modulating immune responses, and communicating with environment. Gut microbiota can be affected by external factors such as foods and antibiotics. Spodoptera frugiperda (Lepidoptera: Noctuidae) is an important destructive pest of grain crops worldwide. The function of gut microbiota in S. frugiperda remains to be investigated. In this study, we fed S. frugiperda larvae with artificial diet with antibiotic mixture (penicillin, gentamicin, rifampicin, and streptomycin) to perturb gut microbiota, and then examined the effect of gut microbiota dysbiosis on S. frugiperda gene expression by RNA sequencing. Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria were the most dominant phyla in S. frugiperda. We found that the composition and diversity of gut bacterial community were changed in S. frugiperda after antibiotics treatment. Firmicutes was decreased, and abundance of Enterococcus and Weissella genera was dramatically reduced. Transcriptome analysis showed that 1,394 differentially expressed transcripts (DETs) were found between the control and antibiotics-treated group. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that antibiotics-induced dysbiosis affected many biological processes, such as energy production, metabolism, and the autophagy–lysosome signal pathway. Our results indicated that dysbiosis of gut microbiota by antibiotics exposure affects energy and metabolic homeostasis in S. frugiperda, which help better understand the role of gut microbiota in insects.

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Section: Methodsmentioning

confidence: 99%

Gut Microbiota Dysbiosis Influences Metabolic Homeostasis in Spodoptera frugiperda

et al. 2021

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“…Such molting behavior in insect larvae is controlled by the steroid hormone ecdysteroids, which we discuss later on [ 34 ]. In adults, cuticular protein is regulated in the testes of most Lepidoptera insects, such as Spodoptera litura [ 35 ]. DEGs also regulate wing development through expression of histidine-rich cuticular protein [ 36 ] in adults.…”

Section: Discussionmentioning

confidence: 99%

Developmental Transcriptome Analysis of Red-Spotted Apollo Butterfly, Parnassius bremeri

Lee¹,

Jesse²,

Veerappan³

et al. 2022

IJMS

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Parnassius bremeri (P. bremeri), a member of the genus Snow Apollo in the swallowtail family (Papilionidae), is a high alpine butterfly that lives in Russia, Korea, and China. It is an endangered wildlife (Class I) in South Korea and is a globally endangered species. The lack of transcriptomic and genomic resources of P. bremeri significantly hinders the study of its population genetics and conservation. The detailed information of the developmental stage-specific gene expression patterns of P. bremeri is of great demand for its conservation. However, the molecular mechanism underlying the metamorphic development of P. bremeri is still unknown. In the present study, the differentially expressed genes (DEGs) across the metamorphic developmental stages were compared using high-throughput transcriptome sequencing. We identified a total of 72,161 DEGs from eight comparisons. GO enrichment analysis showed that a range of DEGs were responsible for cuticle development and the melanin biosynthetic pathway during larval development. Pathway analysis suggested that the signaling pathways, such as the Wnt signaling pathway, hedgehog signaling pathway and Notch signaling pathway, are regulated during the developmental stages of P. bremeri. Furthermore, sensory receptors were also activated, especially during the larval to adult transition stage. Collectively, the results of this study provide a preliminary foundation and understanding of the molecular mechanism in their transcriptomes for further research on the metamorphic development of P. bremeri.

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“…These larvae matured into adults and laid eggs; the larvae were reared in an incubator at 26 ± 1 • C, with a 65 ± 5% relative humidity and a 14:10 h (L:D) photoperiod. The testes from 12 males at days 0, 2, 4, and 6 of the sixth instar larval stage (L6D0, L6D2, L6D4, and L6D6, respectively), as well as days 1 and 3 of the pupal stage (PD1 and PD3, respectively), were collected and washed in PBS for three times, were immediately frozen with liquid nitrogen, and stored at −80 • C until use as outlined in our previous description [38].…”

Section: Experimental Insectsmentioning

confidence: 99%

“…In S. litura, the two kidney-like testes are separated during the larval stages. However, during their transition from final instar larva to pupa the two separated testes fuse to form a single testis [35][36][37][38]. The number of sperm bundles decreases if testicular fusion is artificially blocked by microsurgery.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura

Chang

Jian

et al. 2023

Genes

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Integrin members are cell adhesion receptors that bind to extracellular matrix (ECM) proteins to regulate cell–cell adhesion and cell-ECM adhesion. This process is essential for tissue development and organogenesis. The fusion of two testes is a physiological phenomenon that is required for sperm production and effective reproduction in many Lepidoptera. However, the molecular mechanism of testicular fusion is unclear. In Spodoptera litura, two separated testes fuse into a single testis during the larva-to-pupa transformation. We identified five α and five β integrin subunits that were closely associated with testicular fusion. Integrin α1 and α2 belong to the position-specific 1 (PS1) and PS2 groups, respectively. Integrin α3, αPS1/αPS2, and αPS3 were clustered into the PS3 group. Integrin β1 belonged to the insect β group, and β2, β3, and β5 were clustered in the βν group. Among these integrins, α1, α2, α3, αPS1/PS2, αPS3, β1, and β4 subunits were highly expressed when the testes fused. However, their expression levels were much lower before and after the fusion of the testis. The qRT-PCR and immunohistochemistry analyses indicated that integrin β1 mRNA and the protein were highly expressed in the peritoneal sheath of the testis, particularly when the testes fused. These results indicate that integrins might participate in S. litura testicular fusion.

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Transcriptomic analysis of the testicular fusion in Spodoptera litura

Cited by 10 publications

References 57 publications

Gut Microbiota Dysbiosis Influences Metabolic Homeostasis in Spodoptera frugiperda

Gut Microbiota Dysbiosis Influences Metabolic Homeostasis in Spodoptera frugiperda

Developmental Transcriptome Analysis of Red-Spotted Apollo Butterfly, Parnassius bremeri

Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura

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