Transcriptomic analysis of three Veillonella spp. present in carious dentine and in the saliva of caries-free individuals

Do, Thuy; Sheehy, Evelyn; Mulli, T. K.; Hughes, Francis J.; Beighton, David

doi:10.3389/fcimb.2015.00025

Cited by 43 publications

(39 citation statements)

References 32 publications

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“…In fact, Edlund et al (47) used an in vitro multispecies transcriptomic model to show that the metabolic activities of V. dispar and Veillonella atypica were positively stimulated by the presence of lactic acid and low-pH conditions. Furthermore, a recent metatranscriptomic analysis revealed other potential cariogenic features of Veillonella; for example, V. parvula seems to exhibit distinct intracellular pH control mechanisms, which might explain the preponderance of these species in carious lesions (48). Thus, certain Veillonella species may serve as early indicators of caries activity or even indicators of microbial activities that can promote the initiation of a carious lesion.…”

Section: Discussionmentioning

confidence: 99%

Microbiomes of Site-Specific Dental Plaques from Children with Different Caries Status

et al. 2017

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The oral microbiota associated with the initiation and progression of dental caries has yet to be fully characterized. The Human Oral Microbe Identification Using Next-Generation Sequencing (HOMINGS) approach was used to analyze the microbiomes of site-specific supragingival dental plaques from children with different caries status. Fifty-five children (2 to 7 years of age) were assessed at baseline and at 12 months and grouped as caries free (CF), caries active with enamel lesions (CAE), and caries active with dentin carious lesions (CA). Plaque samples from cariesfree tooth surfaces (PF) and from enamel carious lesions (PE) and dentin carious lesions (PD) were collected. 16S community profiles were obtained by HOMINGS, and 408 bacterial species and 84 genus probes were assigned. Plaque bacterial communities showed temporal stability, as there was no significant difference in beta diversity values between the baseline and 12-month samples. Irrespective of collection time points, the microbiomes of healthy tooth surfaces differed substantially from those found during caries activity. All pairwise comparisons of beta diversity values between groups were significantly different (P Ͻ 0.05), except for comparisons between the CA-PF, CAE-PE, and CA-PE groups. Streptococcus genus probe 4 and Neisseria genus probe 2 were the most frequently detected taxa across the plaque groups, followed by Streptococcus sanguinis, which was highly abundant in CF-PF. Well-known acidogenic/aciduric species such as Streptococcus mutans, Scardovia wiggsiae, Parascardovia denticolens, and Lactobacillus salivarius were found almost exclusively in CA-PD. The microbiomes of supragingival dental plaque differ substantially among tooth surfaces and children of different caries activities. In support of the ecological nature of caries etiology, a steady transition in community species composition was observed with disease progression.

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Section: Discussionmentioning

confidence: 99%

Microbiomes of Site-Specific Dental Plaques from Children with Different Caries Status

et al. 2017

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“…We were the first to show that fecal Veillonella load is associated with lower serum potassium level in this study. Veillonella is known to have a potassium uptake system, which is involved in the maintenance of its cytoplasmic pH . This potassium uptake system might be a possible mechanism explaining the lower serum potassium level in our study.…”

Section: Discussionmentioning

confidence: 52%

Rifaximin‐altered gut microbiota components associated with liver/neuropsychological functions in patients with hepatic encephalopathy: An exploratory data analysis of phase II/III clinical trials

Kawaguchi

Suzuki

Imamura³

et al. 2019

Hepatology Research

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AimsRifaximin (RFX), a non‐systemic antibiotic, improves liver/neuropsychological functions in patients with hepatic encephalopathy (HE). We aimed to investigate the clinical profiles associated with gut bacterial loads using exploratory data analysis and the effects of RFX on the gut microbiota of patients with HE.MethodsWe analyzed the data from 17 patients with HE who underwent fecal microbiota examination in phase II/III trials in Japan. Profiles associated with genera Streptococcus, Veillonella, and Lactobacillus loads were analyzed using classification and regression trees (CART). Changes in gut microbial consortia of seven patients with HE were then assessed 2 weeks after RFX treatment by principal component analysis.ResultsIn the CART, the first and second divergence variables for each higher bacterial load were as follows: (i) in Streptococcus, the number connection test‐A ≥39.55 s and presence of portal‐systemic shunt; (ii) in Veillonella, serum potassium levels <4.75 mEq/L and total cholesterol level <129.5 mg/dL; and (iii) in Lactobacillus, white blood cell counts ≥3.4 × 103/μL and aspartate aminotransferase level ≥44.5 U/L. There was no significant change in total bacterial load before and after RFX treatment; however, there was a decrease in Streptococcus, Veillonella, and Lactobacillus counts after RFX treatment.ConclusionWe report clinical profiles associated with gut bacterial loads in patients with HE, and showed that RFX altered gut microbiota components associated with liver/neuropsychological functions. Thus, RFX could improve liver/neuropsychological functions through the regulation of the gut microbial consortia in patients with HE.

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“…Leptotrichia has a strong glycolysis effect [33], and its detection rate increased in the process of caries [34,35]. Veillonella has a unique intracellular pH control mechanism [36], which might promote the cariogenicity of Streptococcus mutans [35,37]. By inhibition of these bacteria, cariogenicity of the dental plaque was suppressed, microbial new balance was needed and the chance became possible.…”

Section: Discussionmentioning

confidence: 99%

Effect of silver fluoride on micro-ecology of plaque from extensive caries of primary teeth - in vitro study

Liu¹,

Liu

ZHANG

et al. 2020

Preprint

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Background The action mechanism of silver diammine fluoride (SDF) on plaque micro-ecology was seldom studied. This study investigated micro-ecological changes in dental plaque on extensive carious cavity of deciduous teeth after topical SDF treatment. Methods Deciduous teeth with extensive caries freshly removed from school children were collected in clinic. After initial plaque collection, each cavity was topically treated with 38% SDF in vitro. Repeated plaque collections were done at 24 hours and 1 week post-intervention. Post-intervention micro-ecological changes including microbial diversity, microbial metabolism function as well as inter-microbial connections were analyzed and compared after Pyrosequencing of the DNA from the plaque sample using Illumina MiSeq platform. Results After SDF application, microbial diversity decreased (p>0.05). Microbial community composition post-intervention was obviously different from that of supragingival and pre-intervention plaque as well as saliva. At 1 week post-intervention, the relative content of Pseudomonas , Fusobacterium and Pseudoramibacter was obviously higher than before, while most of the other bacteria was obviously reduced, although not statistically significant (P>0.05). The inter-microbial connections became more complex, with positive connections overcame negative ones. Carbohydrate transportation and metabolic functions in the plaque were significantly reduced at 24 hours and 1 week post-intervention. Conclusions SDF has extensive antimicrobial effect on dental plaque, which may reduce carbohydrate metabolism in dental plaque and help promote new balance of the plaque flora.

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Transcriptomic analysis of three Veillonella spp. present in carious dentine and in the saliva of caries-free individuals

Cited by 43 publications

References 32 publications

Microbiomes of Site-Specific Dental Plaques from Children with Different Caries Status

Microbiomes of Site-Specific Dental Plaques from Children with Different Caries Status

Rifaximin‐altered gut microbiota components associated with liver/neuropsychological functions in patients with hepatic encephalopathy: An exploratory data analysis of phase II/III clinical trials

Effect of silver fluoride on micro-ecology of plaque from extensive caries of primary teeth - in vitro study

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