Transcriptomic analysis of two endophytes involved in enhancing salt stress ability of Arabidopsis thaliana

Dong, Zhou-Yan; Rao, Manik Prabhu Narsing; Wang, Hongfei; Fang, Bao‐Zhu; Liu, Yonghong; Li, Li; Xiao, Min; Li, Wenjun

doi:10.1016/j.scitotenv.2019.05.483

Cited by 58 publications

(22 citation statements)

References 38 publications

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“…Transcriptomic analysis that was performed by Dong et al [145] proved that bacterial genes were responsible for water and potassium ion uptake, chlorophyll a reductase, and peptidemethionine (R) -S-oxide reductase were up-regulated when Arabidopsis thaliana grew under salt stress and were inoculated with two endophytic strains (Arthrobacter endophyticus and Nocardiopsis alba). These authors also observed that carotenoid and phenylpropanoid biosynthesis, as well as phenylalanine, glycerolipid, and nitrogen metabolism, played a crucial role in enhancing salt stress tolerance in A. thaliana [145].…”

Section: The Role Of Endophytes In Support Of Defense Mechanisms In Pmentioning

confidence: 99%

Agricultural and Other Biotechnological Applications Resulting from Trophic Plant-Endophyte Interactions

2019

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Endophytic microbiota plays a role not only in supplying plants with the basic nutrients indispensable for their growth, but also helps them in the mechanisms of adaptation to various environmental stresses (i.e., salinity, drought), which is important in the aspect of crop yields. From the agricultural and biotechnological points of view, the knowledge of endophytes and their roles in increasing crop yields, plant resistance to diseases, and helping to survive environmental stress is extremely desirable. This paper reviews some of the beneficial plant–microbe interactions that might be potentially used in both agriculture (plant growth stimulation effect, adaptation of host organisms in salinity and drought conditions, and support of defense mechanisms in plants), and in biotechnology (bioactive metabolites, application of endophytes for bioremediation and biotransformation processes, and production of biofertilizers and biopreparations). Importantly, relatively recent reports on endophytes from the last 10 years are summarized in this paper.

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Section: The Role Of Endophytes In Support Of Defense Mechanisms In Pmentioning

confidence: 99%

Agricultural and Other Biotechnological Applications Resulting from Trophic Plant-Endophyte Interactions

2019

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“…The analysis of novel gene responses of EB may provide a potential method to cope with salinity. EB can enhance Arabidopsis thaliana growth under salt stress and can tolerate high salt concentrations and show a potential competitive advantage of surviving in saline soils [18,19]. The complete genome sequence of the endophyte Bacillus flexus helps in revealing the plant growth-promoting mechanism of EB [20].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium Halomonas sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance

et al. 2020

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Salt stress is an important adverse condition encountered during plant and microbe growth in terrestrial soil ecosystems. Currently, how ice plant (Mesembryanthemum crystallinum) growth-promoting endophytic bacteria (EB) cope with salt stress and regulate growth and the genes responsible for salt tolerance remain unknown. We applied RNA-Seq technology to determine the growth mechanism of the EB Halomonas sp. MC1 strain and the genes involved in salt tolerance. A total of 893 genes were significantly regulated after salt treatment. These genes included 401 upregulated and 492 downregulated genes. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes analysis revealed that the most enriched genes included those related to the outer membrane-bounded periplasmic space, ATPase activity, catabolic process, and proton transmembrane transport. The quantitative real-time polymerase chain reaction data were similar to those obtained from RNA-Seq. The MC1 strain maintained survival under salt stress by regulating cellular and metabolic processes and pyruvate metabolism pathways such as organic and carboxylic acid catabolic pathways. We highlighted the response mechanism of Halomonas sp. MC1 to fully understand the dynamics of complex salt–microbe interactions.

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“…Genomic DNA extraction and PCR amplification of the 16S rRNA gene sequences were performed as described by Li et al [5] and Dong et al [6]. The obtained 16S rRNA gene sequences were compared with available sequences of cultured species in the Ezbiocloud web-based server [7].…”

Section: Full-textmentioning

confidence: 99%

Vulcaniibacterium gelatinicum sp. nov., a moderately thermophilic bacterium isolated from a hot spring

Niu

Rao

Dong

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The present study aimed to determine the taxonomic positions of strains designated R-5-52-3T, R-5-33-5-1-2, R-5-48-2 and R-5-51-4 isolated from hot spring water samples. Cells of these strains were Gram-stain-negative, non-motile and rod-shaped. The strains shared highest 16S rRNA gene sequence similarity with Vulcaniibacterium thermophilum KCTC 32020T (95.1%). Growth occurred at 28–55 °C, at pH 6–8 and with up to 3 % (w/v) NaCl. DNA fingerprinting, biochemical, phylogenetic and 16S rRNA gene sequence analyses suggested that R-5-52-3T, R-5-33-5-1-2, R-5-48-2 and R-5-51-4 were different strains but belonged to the same species. Hence, R-5-52-3T was chosen for further analysis and R-5-33-5-1-2, R-5-48-2 and R-5-51-4 were considered as additional strains of this species. R-5-52-3T possessed Q-8 as the only quinone and iso-C15:0, iso-C11:0, C16 : 0 and iso-C17 : 0 as major fatty acids. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified polar lipids and two unidentified phospholipids. The genomic G+C content was 71.6 mol%. Heat shock proteins (e.g. Hsp20, GroEL, DnaK and Clp ATPases) were noted in the R-5-52-3T genome, which could suggest its protection in the hot spring environment. Pan-genome analysis showed the number of singleton gene clusters among Vulcaniibacterium members varied. Average nucleotide identity (ANI) values between R-5-52-3T, Vulcaniibacterium tengchongense YIM 77520T and V. thermophilum KCTC 32020T were 80.1–85.8 %, which were below the cut-off level (95–96 %) recommended as the ANI criterion for interspecies identity. Thus, based on the above results, strain R-5-52-3T represents a novel species of the genus Vulcaniibacterium , for which the name Vulcaniibacterium gelatinicum sp. nov. is proposed. The type strain is R-5-52-3T (=KCTC 72061T=CGMCC 1.16678T).

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Transcriptomic analysis of two endophytes involved in enhancing salt stress ability of Arabidopsis thaliana

Cited by 58 publications

References 38 publications

Agricultural and Other Biotechnological Applications Resulting from Trophic Plant-Endophyte Interactions

Agricultural and Other Biotechnological Applications Resulting from Trophic Plant-Endophyte Interactions

Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium Halomonas sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance

Vulcaniibacterium gelatinicum sp. nov., a moderately thermophilic bacterium isolated from a hot spring

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