Transcriptomic Analysis Provides Insights into the Differential Effects of Aluminum on Peanut (Arachis hypogaea L.)

Bao, Gegen; Li, Shengyu; Zhou, Qi Tony; Ashraf, Umair; Qiao, Jingxuan; Li, Xin; Wan, Xiaorong; Zheng, Yinan

doi:10.3390/genes13101830

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…According to the transcriptome findings, it was found that several TF families, namely, NAC , BHLH , WRKY , and MYB , which included differentially expressed TFs, performed a strong relationship between As100Glu0 and As100Glu100 (Figure f). These TF families were reported to be related to 2-AP biosynthesis pathway of fragrant rice , and environmental stress of plants. , Particularly, WRKY69, a member of WRKYs was significantly regulated by Glu supplementation in the study which was to construct the root architecture of rice, promoting root development . In addition, WRKY39 , which played a positive role in enhancing salinity tolerance by up-regulating CAT , APX , and GST , was reported .…”

Section: Discussionmentioning

confidence: 91%

“…These TF families were reported to be related to 2-AP biosynthesis pathway of fragrant rice 1,49 and environmental stress of plants. 45,50 Particularly, WRKY69, a member of WRKYs was significantly regulated by Glu supplementation in the study which was to construct the root architecture of rice, promoting root development. 35 In addition, WRKY39, which played a positive role in enhancing salinity tolerance by upregulating CAT, APX, and GST, was reported.…”

Section: ■ Discussionmentioning

confidence: 99%

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l-Glutamate Seed Priming Enhances 2-Acetyl-1-pyrroline Formation in Fragrant Rice Seedlings in Response to Arsenite Stress

Huang,

Deng,

Zhao

et al. 2023

J. Agric. Food Chem.

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2-Acetyl-1-pyrroline (2-AP) is a fragrance compound and flavor in fragrant rice whose precursors are generally glutamate (Glu) and proline (Pro). Our previous study revealed that exogenous Glu enhanced the arsenic (As) tolerance in fragrant rice by improving the ascorbic acid−glutathione cycle and the Pro content in roots. However, less is known about how Glu is involved in 2-AP biosynthesis in fragrant rice under As stress. Herein, a hydroponic experiment of L-Glu seed priming with 0, 100, and 500 μM L-glutamic acid solutions was conducted with two fragrant rice varieties. After that, the 10-day-old seedlings were cultured under 0 and 100 μM arsenite stress for 10 d. Results showed that the 2-AP and Pro contents were increased by 18−30% and 21−78% under As100 μM-Glu100 μM treatment in comparison to the control As100 μM to Glu0 μM, while the activities of pyrroline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (ProDH) were increased by 19−46% and 3−19%, respectively. Furthermore, the 2-AP, Pro contents, and P5CS activity were correlated positively. Correspondingly, a significant abundance of differential expressed metabolites (18) and differential expressed genes (26) was observed in amino acid metabolism and glutathione metabolism pathways. In addition, several essential genes were verified and grouped into the pathways of glutathione metabolism, proline, and arginine metabolism with antioxidant defense system to comodulate 2-AP biosynthesis and stress detoxification. Therefore, the Glu seed priming treatment had a positive impact on the 2-AP biosynthesis of fragrant rice under 100 μM arsenite toxicity.

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Section: Discussionmentioning

confidence: 91%

Section: ■ Discussionmentioning

confidence: 99%

l-Glutamate Seed Priming Enhances 2-Acetyl-1-pyrroline Formation in Fragrant Rice Seedlings in Response to Arsenite Stress

Huang,

Deng,

Zhao

et al. 2023

J. Agric. Food Chem.

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“…Interestingly, the plant hormone signaling pathway was significantly upregulated at higher concentration of Aluminium treatments. Furthermore, the expression of transcription factors (TFs) in peanut is concentration dependent and overall, this study showed that Al at low concentration acts as a stimulant, while at high concentration it is toxic and acted as an inhibitor (Bao et al, 2022a).…”

Section: Cold and Metal Stressmentioning

confidence: 86%

The genus Arachis: an excellent resource for studies on differential gene expression for stress tolerance

Kumar,

Kirti

2023

Front. Plant Sci.

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Peanut Arachis hypogaea is a segmental allotetraploid in the section Arachis of the genus Arachis along with the Section Rhizomataceae. Section Arachis has several diploid species along with Arachis hypogaea and A. monticola. The section Rhizomataceae comprises polyploid species. Several species in the genus are highly tolerant to biotic and abiotic stresses and provide excellent sets of genotypes for studies on differential gene expression. Though there were several studies in this direction, more studies are needed to identify more and more gene combinations. Next generation RNA-seq based differential gene expression study is a powerful tool to identify the genes and regulatory pathways involved in stress tolerance. Transcriptomic and proteomic study of peanut plants under biotic stresses reveals a number of differentially expressed genes such as R genes (NBS-LRR, LRR-RLK, protein kinases, MAP kinases), pathogenesis related proteins (PR1, PR2, PR5, PR10) and defense related genes (defensin, F-box, glutathione S-transferase) that are the most consistently expressed genes throughout the studies reported so far. In most of the studies on biotic stress induction, the differentially expressed genes involved in the process with enriched pathways showed plant-pathogen interactions, phenylpropanoid biosynthesis, defense and signal transduction. Differential gene expression studies in response to abiotic stresses, reported the most commonly expressed genes are transcription factors (MYB, WRKY, NAC, bZIP, bHLH, AP2/ERF), LEA proteins, chitinase, aquaporins, F-box, cytochrome p450 and ROS scavenging enzymes. These differentially expressed genes are in enriched pathways of transcription regulation, starch and sucrose metabolism, signal transduction and biosynthesis of unsaturated fatty acids. These identified differentially expressed genes provide a better understanding of the resistance/tolerance mechanism, and the genes for manipulating biotic and abiotic stress tolerance in peanut and other crop plants. There are a number of differentially expressed genes during biotic and abiotic stresses were successfully characterized in peanut or model plants (tobacco or Arabidopsis) by genetic manipulation to develop stress tolerance plants, which have been detailed out in this review and more concerted studies are needed to identify more and more gene/gene combinations.

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“…In recent years, RNA sequencing (RNA-seq) has emerged as an indispensable tool for transcriptome analysis because it can generate a vast number of reads, providing a comprehensive overview of the transcriptomic landscape. Consequently, RNA sequencing has been applied to investigate Al response mechanisms in various plant species, including Pinus massoniana [42], sugarcane [43], aspen [44], peanut [45], and alfalfa [46], uncovering numerous physiological and metabolic processes involved in Al stress such as oxidative stress, organic acid exudation, defense against cell wall toxicity, and hormone signal transduction. Despite extensive information on Al stress responses, the specific mechanisms via which melatonin regulates Al toxicity in alfalfa remain unexplored.…”

Section: Of 17mentioning

confidence: 99%

Physiological and Transcriptomic Analysis Reveals That Melatonin Alleviates Aluminum Toxicity in Alfalfa (Medicago sativa L.)

Liu,

Cheng,

Wang

et al. 2023

IJMS

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Aluminum (Al) toxicity is the most common factor limiting the growth of alfalfa in acidic soil conditions. Melatonin (MT), a significant pleiotropic molecule present in both plants and animals, has shown promise in mitigating Al toxicity in various plant species. This study aims to elucidate the underlying mechanism by which melatonin alleviates Al toxicity in alfalfa through a combined physiological and transcriptomic analysis. The results reveal that the addition of 5 μM melatonin significantly increased alfalfa root length by 48% and fresh weight by 45.4% compared to aluminum treatment alone. Moreover, the 5 μM melatonin application partially restored the enlarged and irregular cell shape induced by aluminum treatment, resulting in a relatively compact arrangement of alfalfa root cells. Moreover, MT application reduces Al accumulation in alfalfa roots and shoots by 28.6% and 27.6%, respectively. Additionally, MT plays a crucial role in scavenging Al-induced excess H2O2 by enhancing the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), consequently reducing malondialdehyde (MDA) levels. More interestingly, the RNA-seq results reveal that MT application significantly upregulates the expression of xyloglucan endotransglucosylase/hydrolase (XTH) and carbon metabolism-related genes, including those involved in the glycolysis process, as well as sucrose and starch metabolism, suggesting that MT application may mitigate Al toxicity by facilitating the binding of Al to the cell walls, thereby reducing intracellular Al accumulation, and improving respiration and the content of sucrose and trehalose. Taken together, our study demonstrates that MT alleviates Al toxicity in alfalfa by reducing Al accumulation and restoring redox homeostasis. These RNA-seq results suggest that the alleviation of Al toxicity by MT may occur through its influence on cell wall composition and carbon metabolism. This research advances our understanding of the mechanisms underlying MT’s effectiveness in mitigating Al toxicity, providing a clear direction for our future investigations into the underlying mechanisms by which MT alleviates Al toxicity in alfalfa.

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Transcriptomic Analysis Provides Insights into the Differential Effects of Aluminum on Peanut (Arachis hypogaea L.)

Cited by 4 publications

References 48 publications

l-Glutamate Seed Priming Enhances 2-Acetyl-1-pyrroline Formation in Fragrant Rice Seedlings in Response to Arsenite Stress

l-Glutamate Seed Priming Enhances 2-Acetyl-1-pyrroline Formation in Fragrant Rice Seedlings in Response to Arsenite Stress

The genus Arachis: an excellent resource for studies on differential gene expression for stress tolerance

Physiological and Transcriptomic Analysis Reveals That Melatonin Alleviates Aluminum Toxicity in Alfalfa (Medicago sativa L.)

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