Transcriptomic analysis revealing hepcidin expression in Oryzias melastigma regulated through the JAK-STAT signaling pathway upon exposure to BaP

Qian, Chao; Chen, Fang-Yi; Zhang, Min; Wang, Kejian

doi:10.1016/j.aquatox.2018.11.015

Cited by 12 publications

(4 citation statements)

References 43 publications

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“…Hamp1 is found predominantly in the liver of vertebrates and consists of mammalian sequences. The Hamp2 series, however, is contained in several tissues at low concentration [54]. In black rockfish, Hamp1 is upregulated and decreases in reaction to iron overload and infection during anemia and hypoxic conditions, while Hamp2 does not respond to either iron overload or anemia, but is strongly upregulated during bacterial infection and hypoxia [55,56].…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism by which hepcidin controls the iron level in humans primarily includes internalizing and breaking down FPN1 to inhibit the development of intracellular iron, balancing the concentration of extracellular iron and the iron function [54,56]. The membrane protein FPN1 is known primarily for introducing iron into vertebrate cells [57].…”

Section: Discussionmentioning

confidence: 99%

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Role of Hepcidins from Black Rockfish (Sebastes schlegelii) in Iron-Metabolic Function and Bacterial Defense

Lee

Kim

et al. 2020

JMSE

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Hepcidin, an antimicrobial peptide produced by the liver, also controls the iron balance and regeneration in vertebrates. Two types of hepcidin (Hamp1 and Hamp2) have been found in the bodies of black rockfish (Sebastes schlegelii). The full-length cDNA of hepcidin was cloned to enable a study of the antibacterial roles of these two hepcidins (Hamp) in black rockfish. The antimicrobial function of recombinant hepcidins was tested both in vitro and in vivo by the synthesis in Escherichia coli of recombinant hepcidin (approximately 11 kDa) from black rockfish. The recombinant hepcidins inhibited the growth of two bacterial species, Streptococcus iniae FP5228 and Pseudomonas aeruginosa, at various concentrations, in vitro after 6 h post-incubation, respectively. During infection, the production of ferroportin was reduced, suggesting the preservation of iron to prevent microbial proliferation. In vivo administration of Hamp1, but not Hamp2, synthetic peptides induced a substantial reduction in the expression of ferroportin, suggesting that in black rockfish with two forms of hepcidin, ferroportin production is regulated by the iron-regulator Hamp1, and not by the dedicated antimicrobial Hamp2. The findings of this study suggest the various antimicrobial roles of these two types of hepcidin.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Role of Hepcidins from Black Rockfish (Sebastes schlegelii) in Iron-Metabolic Function and Bacterial Defense

Lee

Kim

et al. 2020

JMSE

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“…The JAK-STAT signal pathway is crucial to cellular proliferation, differentiation, and immunomodulation. In medaka (Oryzias melastigma), the JAK-STAT signaling pathway regulates hepcidin1 expression, which exerts an important role associated with pathogen infection or inflammation (Wrighting and Andrews, 2006;Cui et al, 2019). In our study, GIFT tilapia juveniles exposed to LY underwent stress, reflected by the increased T-AOC and COR contents.…”

Section: Discussionmentioning

confidence: 64%

The Welfare of Nile Tilapia (Oreochromis niloticus, GIFT Strain) Juveniles Cultured in Different Light Spectra

Zhai

Wang

et al. 2022

Front. Mar. Sci.

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The light spectrum is a vital environmental factor for the culture of fish, and the welfare of farmed fish is a crucial issue in aquaculture. In this study, Nile tilapia (Oreochromis niloticus, GIFT strain) juveniles were exposed to full-spectrum (LW), red (LR), yellow (LY), or blue (LB) light. After the 45-day experiment, growth performance, stress responses, and aggressive behaviors were evaluated, and transcriptomic analysis was carried out. The results revealed that LW and LR positively affected growth performance. At the same time, LY and LB had a negative effect. Light spectrum induced stress responses of juvenile fish exposed to LY, under which the total antioxidant capacity (T-AOC) and cortisol (COR) contents were the highest. The activities of α-amylase (AMS), protease (PES), and lipase (LPS) in the digestive tract showed a similar tendency, indicating that the light spectra altered the digestive enzyme activities and then affected growth. Behavioral analyses showed increased chase and bite activities of tilapia juveniles exposed to LW and LY. The affected functions included the nervous system, muscle morphogenesis, and immune system-related regulation. Enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways include the tryptophan metabolism signaling pathway, protein digestion and absorption signaling pathway, Jak-STAT signaling pathway, arachidonic acid metabolism signaling pathway, and alpha-linolenic acid metabolism signaling pathway. Overall, light spectra influenced the welfare of farmed tilapia juveniles in terms of growth, stress, and behavior. Our results suggested that LR should be used in juvenile tilapia culture.

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“…Retinol is another effective antioxidant substance that can also enhance the immunity of body. A study involving mice found that RDH8 is capable of converting all-trans retinal to all-trans retinol [ 76 – 78 ],,and it also plays a vital role in aquatic animals [ 79 , 80 ]. However, in this study, we observed a decrease in the methylation level of RDH8 , resulting in reduced gene expression.…”

Section: Discussionmentioning

confidence: 99%

High temperature influences DNA methylation and transcriptional profiles in sea urchins (Strongylocentrotus intermedius)

Liu,

Zeng,

Wang

et al. 2023

BMC Genomics

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Background DNA methylation plays an important role in life processes by affecting gene expression, but it is still unclear how DNA methylation is controlled and how it regulates gene transcription under high temperature stress conditions in Strongylocentrotus intermedius. The potential link between DNA methylation variation and gene expression changes in response to heat stress in S. intermedius was investigated by MethylRAD-seq and RNA-seq analysis. We screened DNA methylation driver genes in order to comprehensively elucidate the regulatory mechanism of its high temperature adaptation at the DNA/RNA level. Results The results revealed that high temperature stress significantly affected not only the DNA methylation and transcriptome levels of S. intermedius (P < 0.05), but also growth. MethylRAD-seq analysis revealed 12,129 CG differential methylation sites and 966 CWG differential methylation sites, and identified a total of 189 differentially CG methylated genes and 148 differentially CWG methylated genes. Based on KEGG enrichment analysis, differentially expressed genes (DEGs) are mostly enriched in energy and cell division, immune, and neurological damage pathways. Further RNA-seq analysis identified a total of 1968 DEGs, of which 813 genes were upregulated and 1155 genes were downregulated. Based on the joint MethylRAD-seq and RNA-seq analysis, metabolic processes such as glycosaminoglycan degradation, oxidative phosphorylation, apoptosis, glutathione metabolism, thermogenesis, and lysosomes are regulated by DNA methylation. Conclusions High temperature affected the DNA methylation and expression levels of genes such as MOAP-1, GGT1 and RDH8, which in turn affects the metabolism of HPSE, Cox, glutathione, and retinol, thereby suppressing the immune, energy metabolism, and antioxidant functions of the organism and finally manifesting as stunted growth. In summary, the observations in the present study improve our understanding of the molecular mechanism of the response to high temperature stress in sea urchin.

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Transcriptomic analysis revealing hepcidin expression in Oryzias melastigma regulated through the JAK-STAT signaling pathway upon exposure to BaP

Cited by 12 publications

References 43 publications

Role of Hepcidins from Black Rockfish (Sebastes schlegelii) in Iron-Metabolic Function and Bacterial Defense

Role of Hepcidins from Black Rockfish (Sebastes schlegelii) in Iron-Metabolic Function and Bacterial Defense

The Welfare of Nile Tilapia (Oreochromis niloticus, GIFT Strain) Juveniles Cultured in Different Light Spectra

High temperature influences DNA methylation and transcriptional profiles in sea urchins (Strongylocentrotus intermedius)

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