Transcriptomic analysis reveals candidate genes associated with anther development in Lilium Oriental Hybrid ‘Siberia’

Dong, Tingting; Wang, Lixuan; Wang, Rui; Xi, Yang; Jia, Wenjie; Yi, Mingfang; Zhou, Xiaofeng; He, Junna

doi:10.3389/fpls.2023.1128911

Cited by 3 publications

(3 citation statements)

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“…Employing advanced high−throughput sequencing analysis, we detected differentially expressed genes within genetically comparable backgrounds. Analysing the shifts in gene expression levels provides a foundational platform for gene function investigation and the analysis of male sterility in plants [6,44]. Further insights drawn from RNA sequencing underscored the 7400 and 10,719 DEGs in HS and HF during the microspore mother stage and tetrad stage, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The signal was monitored by the CFX Connect real−time system (Bio−Rad, Tokyo, Japan) using the 2 −∆∆Ct method. The amount of lily ACTIN in each sample was used to normalize the amount of each target mRNA, with three biological replicates set for each template [6]. The primers used are listed in Supplementary Table S1.…”

Section: Transcriptome Sequencing Of Aborted Pollenmentioning

confidence: 99%

“…As a major commodity flower around the world, lilies are widely planted in China and occupy an enormous market share [1][2][3][4]. Because lily pollen easily contaminates clothing and cleaning the clothing after contamination is inconvenient, lilies sold in the market are generally artificially emasculated via a complex process that wastes considerable manpower and resources and brings considerable trouble to their production [5][6][7]. Therefore, cultivating lilies that are male−sterile and pollen−free is a key direction for breeding in the future.…”

Section: Introductionmentioning

confidence: 99%

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Disruption of the Contents of Endogenous Hormones Cause Pollen Development Obstruction and Abortion in Male-Sterile Hybrid Lily Populations

Jia,

Li,

Wang

et al. 2023

Plants

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Lilies are well−known flowers with large anthers and a high quantity of pollen that easily contaminates clothing and tepals. The anthers need to be artificially removed, leading to production problems. Cultivating male−sterile or pollen−free lilies could solve these problems. The key period of male sterility in a specific male−sterile hybrid lily population was determined through cytological observation. The contents of hormones, soluble sugar, soluble protein, and proline were determined by high−performance liquid chromatography, tandem mass spectrometry and colorimetry. Transcriptome sequencing was used to identify the genes with altered expression. The key period of male sterility was determined to be the microspore mother and tetrad stages. The hormone contents were abnormal in the sterile line compared with the fertile line. The indole−3−acetic acid (IAA) content was higher in the sterile line than in the fertile line at all stages, while the gibberellic acid 4 (GA4) content showed the opposite result. Abscisic acid (ABA) accumulated in the sterile line in both the microspore mother and tetrad stages, and the zeatin riboside (ZR) content in the sterile line increased at the microspore mother stage but decreased at the tetrad stage. The contents of soluble sugar, soluble protein and proline were higher in the fertile line than in the sterile line. Genes involved in auxin and ABA synthesis and signalling pathways were highly expressed in the male−sterile line. Our data suggested that abnormal contents of hormones in the microspore mother and tetrad stages resulted in pollen abortion in a male−sterile hybrid lily population, which indicated that the hormone balance in specific stages plays critical functions in pollen development in lilies.

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Section: Discussionmentioning

confidence: 99%

Section: Transcriptome Sequencing Of Aborted Pollenmentioning

confidence: 99%