Transcriptomic Analysis Reveals Suppression of Photosynthesis and Chlorophyll Synthesis Following Gibberellic Acid Treatment on Oil Palm (Elaies guineensis)

Chai, Sook-Keat; Ooi, Siew–Eng; Ho, Chai Ling; Ong‐Abdullah, Meilina; Chan, Kuang‐Lim; Fitrianto, Anwar; Namasivayam, Parameswari

doi:10.1007/s00344-023-10950-z

Cited by 1 publication

(1 citation statement)

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“…Extensive investigations have revealed the positive roles of IAA, CTK, GA, and SA [ 72 – 75 ], and the negative roles of ETH, JA, and ABA in Chl accumulation [ 76 – 78 ]. However, Previous studies reported a marked decline in the Chl content in oil palm [ 79 ] and Arabidopsis [ 80 ] following GA 3 and IAA treatments, respectively. On the other hand, some studies reported a marked elevation in the Chl level in Chinese cabbage [ 81 ] and citrus [ 82 ] after exogenous application of appropriate doses of ABA and MeJA, respectively.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of GA2ox genes family and analysis of PbrGA2ox1-mediated enhanced chlorophyll accumulation by promoting chloroplast development in pear

Guo,

Liu,

Shen

et al. 2024

BMC Plant Biol

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Background Chlorophyll (Chl) is an agronomic trait associated with photosynthesis and yield. Gibberellin 2-oxidases (GA2oxs) have previously been shown to be involved in Chl accumulation. However, whether and how the PbrGA2ox proteins (PbrGA2oxs) mediate Chl accumulation in pear (Pyrus spp.) is scarce. Results Here, we aimed to elucidate the role of the pear GA2ox gene family in Chl accumulation and the related underlying mechanisms. We isolated 13 PbrGA2ox genes (PbrGA2oxs) from the pear database and identified PbrGA2ox1 as a potential regulator of Chl accumulation. We found that transiently overexpressing PbrGA2ox1 in chlorotic pear leaves led to Chl accumulation, and PbrGA2ox1 silencing in normal pear leaves led to Chl degradation, as evident by the regreening and chlorosis phenomenon, respectively. Meanwhile, PbrGA2ox1-overexpressing (OE) tobacco plants discernably exhibited Chl built-up, as evidenced by significantly higher Pn and Fv/Fm. In addition, RNA sequencing (RNA-seq), physiological and biochemical investigations revealed an increase in abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA) concentrations and signaling pathways; a marked elevation in reducing and soluble sugar contents; and a marginal decline in the starch and sucrose levels in OE plants. Interestingly, PbrGA2ox1 overexpression did not prominently affect Chl synthesis. However, it indeed facilitated chloroplast development by increasing chloroplast number per cell and compacting the thylakoid granum stacks. These findings might jointly contribute to Chl accumulation in OE plants. Conclusion Overall, our results suggested that GA2oxs accelerate Chl accumulation by stimulating chloroplast development and proved the potential of PbrGA2ox1 as a candidate gene for genetically breeding biofortified pear plants with a higher yield.

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