Transcriptomic and biochemical analyses revealed antifungal mechanism of trans-anethole on Aspergillus flavus growth

Lei, Jun-Dong; Li, Qiong; Zhang, Shuai-Bing; Lv, Yang-Yong; Zhai, Huan-Chen; Wei, Shan; Ma, Ping-An; Hu, Yuan-Sen

doi:10.1007/s00253-023-12791-y

Cited by 4 publications

(1 citation statement)

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“…As an important site for the processing of genetic information, ribosomes participate directly in the regulation of cellular protein synthesis and control life activities indirectly, and any changes leading to ribosomal component alterations can directly affect the normal function of ribosomes; thus, ribosomes are a common target of antifungal agents ( Hurtado-Rios et al, 2022 ; Ivanov et al, 2022 ). RNA-seq analysis of P. digitatum , B. cinerea , and Aspergillus flavus revealed that the application of antifungal compounds disrupted the expression of genes related to ribosome biogenesis and protein biosynthesis and impeded protein synthesis ( Roca-Couso et al, 2021 ; Dimkić et al, 2022 ; Lei et al, 2023 ), which is consistent with our results. In addition, the expression of one gene encoding aminoacyl-tRNA synthetase, which catalyzes the binding of transfer (t)RNA to its cognate amino acids to form aminoacyl-tRNA for synthesizing proteins at specific positions of the ribosome, is reduced by B. velezensis CFS treatment.…”

Section: Discussionsupporting

confidence: 91%

Identification of the Ilex macrocarpa anthracnose pathogen and the antifungal potential of the cell-free supernatant of Bacillus velezensis against Colletotrichum fioriniae

Fu,

Wan,

Yang

et al. 2024

Front. Microbiol.

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IntroductionAnthracnose is a significant fungal disease that affects tree growth and development, with Colletotrichum spp. exhibiting host non-specificity and targeting various organs, making disease control challenging.MethodsThis study aimed to identify the pathogenic species causing anthracnose in Ilex macrocarpa in Nanchong, Sichuan Province, and screen effective fungicides, particularly biological ones. The pathogen was identified as Colletotrichum fioriniae through morphological observation, pathogenicity assays, and molecular biological methods. Three biological and five chemical fungicides were evaluated for their effects on the mycelial growth and spore germination rate of the pathogen.ResultsThe results indicated that prochloraz was the most effective chemical fungicide, while the cell-free supernatant (CFS) of Bacillus velezensis had the most significant inhibitory effect among the biological fungicides. Transcriptome analysis revealed that the CFS of B. velezensis significantly reduced the expression of genes associated with ribosomes, genetic information processing, membrane lipid metabolism, and sphingolipid biosynthesis in C. fioriniae. Additionally, the glutathione pathway’s expression of various genes, including key genes such as GST, GFA, Grx, TRR, and POD, was induced. Furthermore, the expression of 17 MFS transporters and 9 ABC transporters was increased. Autophagy-related ATGs were also affected by the B. velezensis CFS.DiscussionThese findings suggest that the B. velezensis CFS may inhibit C. fioriniae through interference with ribosomes, genetic information processing, cell membrane metabolism, and energy metabolism. These results provide potential target genes for the B. velezensis CFS and insights into the antifungal mechanism by which B. velezensis inhibits C. fioriniae.

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