Transcriptomic and metabolic response to chronic and acute thermal exposure of juvenile geoduck clams Panopea globosa

Juárez, Óscar E.; Cruz, Fabiola Lafarga-De la; Leyva-Valencia, Ignacio; López-Landavery, Edgar A.; García-Esquivel, Zaúl; Dı́az, Fernando; Re-Araujo, Denisse; Vadopalas, Brent; Galindo-Sánchez, Clara E.

doi:10.1016/j.margen.2018.09.003

Cited by 25 publications

(9 citation statements)

References 91 publications

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“…Furthermore, we found that C. angulata had higher contents of arginine and histidine than did C. gigas (Supplementary Table 8). The oxygen consumption rate is a physiological and metabolic indicator frequently used to reflect aerobic respiration and energy metabolism levels (Prins et al, 1997), and as a basic physiological activity of energy metabolism, it indicates the physiological state of organisms and the influence of environmental factors (Lannig et al, 2006;Sokolova et al, 2012;Juárez et al, 2018). Therefore, we suggest that the higher normalized oxygen consumption rate in C. angulata than in C. gigas reflects its stronger aerobic metabolism capacity and higher allocation of energy to growth.…”

Section: Crassostrea Angulata Allocates More Energy To Growthmentioning

confidence: 77%

Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata

Wang

et al. 2021

Front. Mar. Sci.

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Understanding the genetic basis of trait variations and their coordination between relative species or populations distributing in different environmental conditions is important in evolutionary biology. In marine ectotherms, growth-defense trade-offs are a common ecological and evolutionary phenomenon. However, the biochemical and molecular mechanisms that govern these trade-offs in marine ectotherms in the evolutionary perspective remain poorly investigated. Oysters are among the most important species in global aquaculture. Crassostrea gigas (C. gigas) and Crassostrea angulata (C. angulata) are two allopatric congeneric dominant oyster species that inhabit the northern and southern intertidal areas of China. Wild C. gigas and C. angulata were spawned, and their F1 progeny were cultured in the same sites to reduce the environmental effects. Untargeted metabolomics and transcriptomics, together with phenotypic parameters including morphological traits (growth performance), nutritional content (glycogen, crude fat, and fatty acid content), physiology (normalized oxygen consumption rate and total antioxidant capacity) were applied to assess metabolic and transcript divergences between C. gigas and C. angulata. Integrated analyses of metabolites and transcriptomes showed that C. gigas allocated more energy to storage and defense by suppressing glycolysis, fatty acid oxidation and by upregulating fatty acid synthesis, antioxidant gene expression, and related metabolites. The metabolic and transcript results were further confirmed by the phenotypic data that C. gigas has higher glycogen and crude fat content and fatty acid unsaturation and stronger antioxidant capacity than C. angulata. In contrast, C. angulata exhibited better growth performance and a higher oxygen consumption rate. These findings suggest that C. angulata allocates more energy to growth, which is embodied in its stronger aerobic capacity and higher levels of protein synthesis genes, metabolites, and growth-related biomarkers. This study will help to enlighten the evolutionary patterns and genetic basis of growth-defense trade-offs in marine ectotherms and the biochemical and molecular mechanisms underlying energy allocation. Also, the key genes and metabolites of glycogen and fatty acids pathway identified in this study will be applied for meat quality improvement in the oyster industry.

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Section: Crassostrea Angulata Allocates More Energy To Growthmentioning

confidence: 77%

Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata

Wang

et al. 2021

Front. Mar. Sci.

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“…Moreover, investigating the correlation between individual-level responses and molecular changes is useful toward a better understanding of the responses and regulating mechanisms from an overall perspective. Recently, research has increasingly focused on molecular adaptation or tolerance of marine bivalves to heat stress, and new analytic techniques, such as transcriptomics (Lim et al, 2016;Nie et al, 2017;Yang et al, 2017;Juárez et al, 2018;Zhang et al, 2019) and metabolomics (Ellis et al, 2014;Digilio et al, 2016), were used. The mantle tissue of mollusks has multiple functions, which include ligament secretion and sensorial activities; moreover, this tissue is very responsive to external stimuli (Artigaud et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Jiang

Jiao

Sun

et al. 2020

PeerJ

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Marine mollusks are commonly subjected to heat stress. To evaluate the effects of heat stress on the physiological metabolism of the ark shell Scapharca subcrenata, clams were exposed to different high temperatures (24, 28 and 32 °C) for 72 h. The oxygen consumption and ammonia excretion rates were measured at 2, 12, 24, 48 and 72 h. The results indicated that the metabolic rates of the ark shell significantly increased with increasing heat stress, accompanied by mortalities in response to prolonged exposure. A metabolomics approach based on gas chromatography coupled with mass spectrometry was further applied to assess the changes of metabolites in the mantle of the ark shell at 32 °C. Moreover, multivariate and pathway analyses were conducted for the different metabolites. The results showed that the heat stress caused changes in energy metabolism, amino acid metabolism, osmotic regulation, carbohydrate metabolism and lipid metabolism through different metabolic pathways. These results are consistent with the significant changes of oxygen consumption rate and ammonia excretion rate. The present study contributes to the understanding of the impacts of heat stress on intertidal bivalves and elucidates the relationship between individual-level responses and underlying molecular metabolic dynamics.

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“…Acute thermal stress experiments provide a tool to identify and predict tolerance to stress using large sample sizes across environmental gradients 14 . In the marine environment, such experiments have been used to investigate heat stress thresholds in metabolic 15 , molecular 16,17 , and/or behavioural [18][19][20] traits across a variety of marine vertebrates and invertebrates. These various approaches have identi ed heat-tolerant corals after exposure to acute thermal stress [21][22][23][24][25][26] .…”

Section: Introductionmentioning

confidence: 99%

Experimental considerations of acute heat stress assays to quantify coral thermal tolerance

Nielsen

Matthews

Frith

et al. 2022

Preprint

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Thermal tolerance is variable in corals, yet intrinsic and extrinsic drivers of tolerance are not well understood. Understanding the distribution and abundance of heat tolerant corals across seascapes is imperative for predicting responses to climate change and to support novel management actions. Rapid and high-throughput methods to measure heat-induced coral bleaching sensitivity are increasingly required to understand current and predict future responses to climate change. Experimental evaluations of coral heat and bleaching tolerance typically involve ramp-and-hold experiments run across days to weeks within aquarium facilities with limits to colony replication. Field-based acute heat stress assays have emerged as an alternative experimental approach to rapidly quantify heat tolerance in a large number of samples yet the role of key methodological considerations on the stress response measured remains unresolved. Here, we quantify the effects of coral fragment size, sampling time point, and physiological measures on the acute heat stress response in adult corals. The effect of fragment size differed between species (Acropora tenuis and Pocillopora damicornis). Most physiological parameters measured here declined over time (tissue colour, chlorophyll-a and protein content) from the onset of heating, with the exception of maximum photosynthetic efficiency (Fv/Fm), which was stable up to 24h post heating. Based on our experiments, we identified photosynthetic efficiency, tissue colour change, and host-specific assays such as catalase activity as key physiological measures for rapid quantification of thermal tolerance. We recommend that future applications of acute heat stress assays include larger fragments (>9cm2) where possible and sample between 10 - 14h after the end of heat stress. A validated high-throughput experimental approach combined with cost-effective genomic and physiological measurements underpins the development of markers and maps of heat tolerance across seascapes and ocean warming scenarios.

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Transcriptomic and metabolic response to chronic and acute thermal exposure of juvenile geoduck clams Panopea globosa

Cited by 25 publications

References 91 publications

Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata

Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Experimental considerations of acute heat stress assays to quantify coral thermal tolerance

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