Transcriptomic and metabolomic adaptation of Nannochloropsis gaditana grown under different light regimes

Patelou, Maria; Infante, Carlos; Dardelle, Flavien; Randewig, Dörte; Kouri, Evangelia D.; Udvardi, Michael K.; Tsiplakou, Eleni; Mantecón, Lalia; Flemetakis, Emmanouil

doi:10.1016/j.algal.2019.101735

Cited by 40 publications

(17 citation statements)

References 96 publications

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“…After switching from red light to blue light, the turnover rate of PK in P. tricornutum increased, while switching from blue light to red light decreased PK activity. Conversely, in Nannochloropsis gaditana , red filtered light strongly enhanced the concentration of amino acids, especially asparagine (13-fold increase) [ 38 ]. Pyruvate carboxylase activity also increased, consistent with active amino acid biosynthesis under red light and the need to replenish TCA intermediates during this process.…”

Section: Discussionmentioning

confidence: 99%

Effect of Light Intensity and Wavelength on Biomass Growth and Protein and Amino Acid Composition of Dunaliella salina

Sui

Harvey

2021

Foods

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Dunaliella salina is a halotolerant, photoautotrophic marine microalga and one of the richest sources of natural carotenoids but also shows potential as a novel food source with high protein quality. This study sought to optimise the production of biomass, protein and amino acids from D. salina, alongside carotenoids using a two-stage cultivation approach based on the use of light of different intensities and quality, i.e., white, red and blue LED light. In stage 1, four white LED light intensities were tested. In stage 2, the same four light intensities from either blue or red LEDs were applied once exponential growth ceased and cells reached the stationary phase under white LED light in stage 1. Remarkably, both biomass concentration and biomass productivity showed a 1.3–1.7-fold increase in stage 2, without medium replenishment, while protein concentration and protein productivity showed an ~1.1-fold increase. The amino acid content and amino acid index remained unchanged from stage 1 to stage 2, and minimum difference was found across different light intensities. Overall, D. salina delivered so-called high protein quality, with an essential amino acid index (EAAI) of 0.99, and red light, which has previously been shown to increase carotenoid production, boosted further biomass production over and above white light, at all light intensities tested.

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Section: Discussionmentioning

confidence: 99%

Effect of Light Intensity and Wavelength on Biomass Growth and Protein and Amino Acid Composition of Dunaliella salina

Sui

Harvey

2021

Foods

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“…In Tetraselmis suecica , a set of 14 selected genes exhibited significant up- and down-regulation using RT-qPCR in agreement with RNA-seq results [ 58 ]. Recently, a RT-qPCR platform containing more than 100 targeted genes was used for Nannochloropsis gaditana to study the influence of light quality on metabolism [ 71 ]. A similar strategy with an array of ~100 genes was also employed in human cell lines to screen for the potential bioactivity of microalgae extracts [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui

Torres¹,

Lama²,

Mantecón³

et al. 2021

PLoS ONE

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Quantitative real-time reverse transcription PCR (RT-qPCR) is a highly sensitive technique that can be applied to analyze how genes are modulated by culture conditions, but identification of appropriate reference genes for normalization is a critical factor to be considered. For this reason, the expression stability of 18 candidate reference genes was evaluated for the green microalgae Tetraselmis chui using the widely employed algorithms geNorm, NormFinder, BestKeeper, the comparative ΔCT method, and RefFinder. Microalgae samples were collected from large scale outdoor photobioreactors during the growing phase (OUT_GP), and during the semi-continuous phase at different times of the day (OUT_DC). Samples from standard indoor cultures under highly controlled conditions (IND) were also collected to complement the other data. Different rankings for the candidate reference genes were obtained depending on the culture conditions and the algorithm employed. After comparison of the achieved ranks with the different methods, the references genes selected for samples from specific culture conditions were ALD and EFL in OUT_GP, RPL32 and UBCE in OUT_DC, and cdkA and UBCE in IND. Moreover, the genes EFL and cdkA or EFL and UBCE appeared as appropriate combinations for pools generated from all samples (ALL). Examination in the OUT_DC cultures of genes encoding the large and small subunits of ADP-glucose pyrophosphorylase (AGPL and AGPS, respectively) confirmed the reliability of the identified reference genes, RPL32 and UBCE. The present study represents a useful contribution for studies of gene expression in T. chui, and also represents the first step to set-up an RT-qPCR platform for quality control of T. chui biomass production in industrial facilities.

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“…On the other hand, the content of desirable substances (e.g., PUFAs) in microalgae can be modified by external factors such as temperature or light intensity during cultivation [ 59 , 63 ]. Furthermore, the nutrient profile can be altered by engineering a number of enzymes (desturases and elongases) and transcription factors [ 64 ] or by filtering light [ 65 ]. Furthermore, the selection of microalgal species plays a role because some strains from the Nannochloropsis , Phaeodactylum , Schizochytrium , Arthrospira , and Thraustochytrium genera can accumulate high contents of EPA, DHA, and/or γ-linolenic acid [ 59 , 63 , 66 , 67 , 68 ].…”

Section: Alternative and Unconventional Feeds Used In Dairy Dietsmentioning

confidence: 99%

Alternative and Unconventional Feeds in Dairy Diets and Their Effect on Fatty Acid Profile and Health Properties of Milk Fat

Hadrová

Sedláková

Křížová

et al. 2021

Animals

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Milk fat is an important nutritional compound in the human diet. From the health point of view, some fatty acids (FAs), particularly long-chain PUFAs such as EPA and DHA, have been at the forefront of interest due to their antibacterial, antiviral, anti-inflammatory, and anti-tumor properties, which play a positive role in the prevention of cardiovascular diseases (CVD), as well as linoleic and γ-linolenic acids, which play an important role in CVD treatment as essential components of phospholipids in the mitochondria of cell membranes. Thus, the modification of the FA profile—especially an increase in the concentration of polyunsaturated FAs and n-3 FAs in bovine milk fat—is desirable. The most effective way to achieve this goal is via dietary manipulations. The effects of various strategies in dairy nutrition have been thoroughly investigated; however, there are some alternative or unconventional feedstuffs that are often used for purposes other than basic feeding or modifying the fatty acid profiles of milk, such as tanniferous plants, herbs and spices, and algae. The use of these foods in dairy diets and their effects on milk fatty acid profile are reviewed in this article. The contents of selected individual FAs (atherogenic, rumenic, linoleic, α-linolenic, eicosapentaenoic, and docosahexaenoic acids) and their combinations; the contents of n3 and n6 FAs; n6/n3 ratios; and atherogenic, health-promoting and S/P indices were used as criteria for assessing the effect of these feeds on the health properties of milk fat.

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Transcriptomic and metabolomic adaptation of Nannochloropsis gaditana grown under different light regimes

Cited by 40 publications

References 96 publications

Effect of Light Intensity and Wavelength on Biomass Growth and Protein and Amino Acid Composition of Dunaliella salina

Effect of Light Intensity and Wavelength on Biomass Growth and Protein and Amino Acid Composition of Dunaliella salina

Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui

Alternative and Unconventional Feeds in Dairy Diets and Their Effect on Fatty Acid Profile and Health Properties of Milk Fat

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