Transcriptomic and Metabolomic Analysis Unravels the Molecular Regulatory Mechanism of Fatty Acid Biosynthesis in Styrax tonkinensis Seeds under Methyl Jasmonate Treatment

Yu, Wanwen; Chen, Hong; Han, Chao; Liu, Zemao; Ni, Ming; Wu, Qikui; Yu, Fangyuan

doi:10.3390/ijms23116190

Cited by 4 publications

(5 citation statements)

References 64 publications

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“…The duration of the seed development influences oil content and fatty acid composition as well as genotype and environment. The maximum crude fat (CF) mass fraction was found in S. tonkinensis seeds after methyl jasmonate (MJ) application at 70 DAF and the ACC activity displayed a similar trend to that of the CF mass fraction [22]. The high oil content in this study was seed stage 4 WAF, which was consistent with the high expression of the accB gene in mature seeds.…”

Section: Discussionsupporting

confidence: 80%

Expression of Acetyl Coa Carboxylase Gene from Cleome Viscosa L. and Its Seed Oil

Jenpromrak,

Klinthanom,

Chaiprasongsuk

et al. 2023

ijmst

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Asian spider flower (Cleome viscosa L.) is annual herbaceous weed plant. Its seed oil has similar properties to the seed oil from Jatropha curcas L. which is the biodiesel plant. This research focused on the expression of acetyl CoA carboxylase gene in 4 stages of seeds (1-4 weeks after fruit set (WAF)) by Reverse transcriptase Polymerase Chain Reaction (RT-PCR). Acetyl CoA carboxylase (ACCase) is the essential enzyme for the first step in the biosynthesis of long-chain fatty and glycerol. The heteromeric ACCase consists of 4 subunits. accA, accB and accC gene were located in nuclear, while the accD gene was in chloroplast. The result showed that the expression of accA gene showed the most highly expressed in 3 WAF, while the expression of accC and accD showed the most highly expression in 2-3 WAF. These results indicated that co-expression of accA, accC and accD were highest in 3 WAF and down regulated in 4 WAF. In contrast, accB gene showed the high expression level in 4 weeks after fruit set. The total oil was obtained by petroleum ether soxhlet extraction. The high oil content was 3-4 WAF. These findings suggested that comparatively high expression of the Accase gene related to the persistence of oil accumulation during the late stage of seed oil formation for C. viscosa L.

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Section: Discussionsupporting

confidence: 80%

Expression of Acetyl Coa Carboxylase Gene from Cleome Viscosa L. and Its Seed Oil

Jenpromrak,

Klinthanom,

Chaiprasongsuk

et al. 2023

ijmst

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“…However, K1 treatment increased the ACCase activity of seeds to a greater extent than K2 and K3 treatments. The possible reasons for this are as follows: Although potassium is necessary for the growth and development of plants, too much of it can have adverse consequences on the plants, including functional abnormalities and the excessive creation of reactive oxygen species(ROS) (Chen, Chen, Han, Liu, Ni, et al, 2022; Johnson et al, 2022; Xu et al, 2011). In fact, plants need their cytoplasmic K concentration to be maintained within a certain range (Xu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Determination of seed oil content: The Soxhlet extraction method was used (Chen, Chen, Han, Liu, Ni, et al, 2022). In brief, the seeds were first baked in an oven at 65°C to a constant weight.…”

Section: Test Methodsmentioning

confidence: 99%

Foliar spraying of potassium gluconate promotes the synthesis of the seed oil of Styrax tonkinensis

Zheng

Xie

Liu

et al. 2023

J Americ Oil Chem Soc

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Styrax tonkinensis is a promising woody biodiesel species. In this experiment, we investigated how foliar spraying of potassium gluconate solution affected the production of seed oil in S. tonkinensis. Leaf spraying of potassium gluconate solution appreciably promoted the photosynthesis of S. tonkinensis leaves through both the light reaction and the dark reaction. By boosting the activity of enzymes involved in glucose metabolism and seed oil synthesis, foliar spraying of potassium gluconate solution also dramatically enhanced glucose metabolism and seed oil synthesis in the S. tonkinensis seeds. This indicates that foliar spraying with potassium gluconate solution discernibly improved the synthesis of seed oil in S. tonkinensis by encouraging the transit of photosynthetic products from the source to the reservoir and the flow of carbon into the seed oil synthesis in the seeds. Overall, the medium concentration of potassium gluconate solution (4 g/L) was found to be the most effective for increasing the oil content of S. tonkinensis seeds. While higher potassium gluconate solution concentrations (6 g/L) had a slight inhibitory effect on specific indicators (ACCase) during specific seed development periods. This study highlighted the importance of potassium for seed oil synthesis in woody plants and provided a useful foundation for future cultivation practices aimed at enhancing seed oil synthesis in woody plants.

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“…Styrax tonkinensis (Pierre) Craib ex Hartw is a fast-growing tropical and subtropical tree species native to Southeast Asia and South China in the genus Styracaceae [7]. It is a great biomass energy tree with oil, medicinal, and wood benefits.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Provides Insights into Lignin Biosynthesis in Styrax tonkinensis Branches

Han,

Xu,

Chen

et al. 2024

Forests

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Approximately 12% of China’s papermaking raw materials are derived from wood, while the majority are sourced from branches. Styrax tonkinensis is a more desirable species for pulpwood in subtropical ultra-short rotations, whose branches are prone to breakage. Lignin has a significant impact on wood quality and pulping yield, and the growth process influences lignin biosynthesis. To explore the lignin biosynthesis pathway in S. tonkinensis, we determined the lignin content in the current-year and biennial branches on 20 July, 20 September, and 20 October and analyzed the transcriptome sequencing results. It was concluded that the lignin content showed an increasing trend in the current-year branches (182.26, 206.17, and 213.47 mg/g, respectively), while that in the biennial branches showed a decrease in the samples taken in October, without significant difference (221.77, 264.43, and 261.83 mg/g, respectively). The transcriptome sequencing results showed that 91,513 unigenes were spliced with a total length of 92,961,618 bp. KEGG pathway analysis indicated that the upregulated DEGs were mainly enriched in the phenylpropanoid biosynthesis pathway. Our study suggested that CCoAOMT, COMT, peroxidase, and F5H may serve as key enzymes regulating lignin synthesis in branches of S. tonkinensis, thereby influencing the lignin content.

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Transcriptomic and Metabolomic Analysis Unravels the Molecular Regulatory Mechanism of Fatty Acid Biosynthesis in Styrax tonkinensis Seeds under Methyl Jasmonate Treatment

Cited by 4 publications

References 64 publications

Expression of Acetyl Coa Carboxylase Gene from Cleome Viscosa L. and Its Seed Oil

Expression of Acetyl Coa Carboxylase Gene from Cleome Viscosa L. and Its Seed Oil

Foliar spraying of potassium gluconate promotes the synthesis of the seed oil of Styrax tonkinensis

Transcriptome Analysis Provides Insights into Lignin Biosynthesis in Styrax tonkinensis Branches

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