Transcriptomic and metabolomic profiling of chicken adipose tissue in response to insulin neutralization and fasting

Ji, Bo; Ernest, Ben; Gooding, Jessica; Das, Suchita; Saxton, Arnold M.; Simon, Jean; Dupont, Joëlle; Métayer-Coustard, Sonia; Campagna, Shawn R.; Voy, Brynn H.

doi:10.1186/1471-2164-13-441

Cited by 90 publications

(94 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several recent studies have investigated the regulatory mechanisms of chicken adipogenesis using genome-wide analysis of mRNA (Ji et al 2012; Regassa and Kim 2015) and microRNA (Wang et al 2015). However, little is known about the regulatory mechanisms of adipogenesis.…”

mentioning

confidence: 99%

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Zhang

Han

et al. 2017

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

Long noncoding RNAs (lncRNAs) regulate adipogenesis and other processes associated with metabolic tissue development and function. However, little is known about the function and profile of lncRNAs during preadipocyte differentiation in the chicken (Gallus gallus). Herein, lncRNA and mRNA expression in preadipocytes at different stages of differentiation were analyzed using RNA sequencing. A total of 1,300,074,528 clean reads and 27,023 novel lncRNAs were obtained from 12 samples. The number of genes (1336 lncRNAs and 1759 mRNAs; 3095 in total) differentially expressed across various stages declined as differentiation progressed. Differentially expressed genes were found to be involved in several pathways related to preadipocyte differentiation that have been extensively studied, including glycerolipid metabolism, and the mammalian target of rapamycin, peroxisome proliferator-activated receptor, and mitogen-activated protein kinase signaling pathways. To our knowledge, some pathways are being reported for the first time, including the propanoate metabolism, fatty acid metabolism, and oxidative phosphorylation pathways. Furthermore, 3095 differentially expressed genes were clustered into eight clusters, and their expression patterns were determined through K-means clustering. Genes involved in the K2 cluster likely play important roles in preadipocyte differentiation. Six stage-specific modules related to A0 (day 0), A2 (day 2), and A6 (day 6) stages were identified, using weighted coexpression network analysis. Nine central, highly connected .genes in stage-specific modules were subsequently identified, including XLOC_068731, XLOC_022661, XLOC_045161, XLOC_070302, CHD6, LLGL1, NEURL1B, KLHL38, and ACTR6. This study provides a valuable resource for further study of chicken lncRNA and facilitates a better understanding of preadipocyte differentiation in the chicken

show abstract

mentioning

confidence: 99%

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Zhang

Han

et al. 2017

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

show abstract

“…Fatty acid esterification enhances TG synthesis and may be associated with increased content of muscle fat (Ji et al, 2012). Lysophosphatidic acid is further acylated at the sn-2 position by AGPAT to form phosphatidic acid (Bitou et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Expression of Lipid Metabolism-Associated Genes in Male and Female White Feather Chicken

YiJun

et al. 2016

J. Poult. Sci.

View full text Add to dashboard Cite

Differential lipid metabolic requirements of sexually-mature males and females may influence the regulation of lipid metabolism-associated genes and hence the content of adipose tissue. We measured the expression of eight lipid metabolism-associated genes (fatty acid synthase, FASN; acylglycerol-3-phosphate O-acyltransferase 9, AGPAT9; peroxisomal proliferator-activated receptor γ, PPARγ; lipoprotein lipase, LPL; carnitine palmitoyl transferase 1 A, CPT1A; carnitine palmitoyl transferase 1 B, CPT1B; acyl-COA dehydrogenase long chain, ACADL; monoglyceride lipase, MGL) in eight tissues (hypothalamus, HYP; liver; heart; pectoralis major muscle, PM; gastrocnemius muscle, GAS; abdominal fat, AF; clavicular fat, CF; subcutaneous fat, SF) of five male and five female white feather chickens using real time PCR at 217 d (when the females were at peak egg production). There were no difference between sexes, nor were there sex by tissue interactions for CPT1A and MGL. In both cases expression was greater for liver than the other tissues. When interactions of sex by tissue were significant, the FASN mRNA abundance in HYP, liver, and PM was greater for females than males. There was no sexual dimorphism for any tissue for PPARγ. Overall values were greater for adipose depots than HYP and liver with muscles intermediate for AGPAT9. LPL mRNA abundance in PM and AF was greater for females than males, with the pattern reversed for heart and SF. CPT1B mRNA abundance in GAS and CF was greater for females than males, with the relationship reversed for liver. ACADL mRNA abundance in HYP, liver, and GAS was greater for females than males, and lower in PM than males. The results demonstrated that expression of lipid metablism-associated genes varies among sexes in mature chickens depending on the gene and the tissue.

show abstract

“…Moreover, growth of the abdominal WAT in chickens is from a combination of adipose cell hyperplasia (increase in adipocyte number) and hypertrophy (increase in adipocyte volume) up to about 12 to 14 weeks, then it continues mainly by hypertrophy (Ji et al, 2012). The endocrine role of avian WAT remains enigmatic as many of the classical hormones found in mammalian adipose tissue have not been found in avians (Ramachandran et al, 2013).…”

Section: Endocrine Function Of Adipose Tissue and Its Implications Fomentioning

confidence: 99%

“…Relatively little is known about regulation of adipose tissue deposition and metabolism in chickens (Ji et al, 2012). Most studies have focused on broilers, and have shown that adipose tissue metabolism in the chicken is regulated by energy status and, to a lesser extent by insulin.…”

Section: Regulation Of Lipid Metabolism In the Laying Henmentioning

confidence: 99%

Fatty Liver Haemorrhagic Syndrome in Laying Hens: Field and Experimental Investigations

Shini¹

View full text Add to dashboard Cite

Field and experimental investigations were conducted to study the fatty liver haemorrhagic syndrome (FLHS) in caged laying hens. The main goal of the research was to gain a greater understanding of the aetiology and pathogenesis of this non-infectious disease. The disease is characterized by excessive accumulation of fat in the liver and abdominal cavity, subsequent liver rupture, haemorrhage and sudden death of hens. It has been shown that the balance between hepatic synthesis and secretion of lipids is the key point that regulates hepatic and extrahepatic fat deposition in hens. Liver fat accumulation can be increased by many factors, especially nutrition, housing conditions, and inflammatory challenges. A description of normal and abnormal lipid metabolism in the hen, and consequences for hen health and disease (including FLHS) is given in the literature review (Chapter 2).The initial study examined mortality of layer flocks kept in three different housing systems (cage, barn and free range) on the Gatton Campus, University of Queensland (Chapter 4). It was shown that there were no significant differences in mortality rates of hens (6.1%, 6.4% and 5.8%, for cages, barns and free range, respectively) between the housing systems but the causes of death were different. The most common cause of death in hens kept in cages was FLHS with 74% of dead hens dying from the condition. In contrast, FLHS only accounted for 0 to 5% of hen mortality in the other systems. Post-mortem of dead hens and body weight monitoring of flocks throughout the laying cycle were recommended as tools to predict FLHS.An epidemiological survey (Chapter 5) of caged flocks in South-Eastern Queensland was undertaken to explore the prevalence of FLHS in commercial layer flocks. From necropsies of dead birds from 7 flocks of different ages, it was found that approximately 40% of hens died due to FLHS. This result indicated that FLHS continues to be the major cause of mortality in commercial caged laying hens kept in either controlled environment sheds or naturally ventilated sheds. As part of the epidemiological study, a questionnaire was circulated among farms and the responses revealed that there is a general lack of knowledge of FLHS in the industry.The study of the pathogenesis of FLHS is very difficult as it occurs sporadically and over an extended period of time in the field. In the studies in Chapter 6, an oestradiol (E 2 ) hen model was used to study the condition. The administration of exogenous E 2 reproduced the disease in 30 wk old laying hens, and was associated with significant changes in E 2 plasma levels and metabolic iv profiles, increased liver weights, and macroscopic (fat depositions, haemorrhages and haematomas) and microscopic alterations. Hens exposed to E 2 and fed ad libitum diet experienced severe FLHS and had a higher incidence of FLHS than hens that had their feed intake restricted by 10%. One interesting observation from this study (not reported from other investigators) was the alteration of total leukocyte n...

show abstract

Transcriptomic and metabolomic profiling of chicken adipose tissue in response to insulin neutralization and fasting

Cited by 90 publications

References 62 publications

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Expression of Lipid Metabolism-Associated Genes in Male and Female White Feather Chicken

Fatty Liver Haemorrhagic Syndrome in Laying Hens: Field and Experimental Investigations

Contact Info

Product

Resources

About