Transcriptomic and metabolomic profiling of a Rhodotorula color mutant to improve its lipid productivity in fed-batch fermentation

Zhao, Yihan; Yu, Zhongxin; Fu, Rao; Zhang, Tao; Li, Jing; Zhang, Jianfa

doi:10.1007/s11274-021-03043-0

Cited by 7 publications

(7 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transcriptome analysis has been used in R. toruloides studies to find functional genes and promoters [27,28] and to elucidate the effect of carbon sources on the fermentation performance. [22,29] Here Nucleolus and ribosome activities were affected subsequently, which resulted in a profound impact on the overall biological processes.…”

Section: Discussionmentioning

confidence: 99%

“…Transcriptome analysis has been used in R. toruloides studies to find functional genes and promoters [ 27,28 ] and to elucidate the effect of carbon sources on the fermentation performance. [ 22,29 ] Here transcriptome analysis explained the inhibitory effect of methanol on the growth of TO2. Methanol impaired the function of membrane transport especially the activity of glycerol transport.…”

Section: Discussionmentioning

confidence: 99%

“…Then preparation of the samples and 1 H NMR analysis were carried out according to our previous method. [ 22 ] Identification of metabolites was based on the characteristic chemical shifts from the publicly accessible metabolomics databases.…”

Section: Methodsmentioning

confidence: 99%

“…Biomass was determined by weighing the cell residue after drying. [ 22 ] Lipid concentration was determined by the phosphate‐vanillin method, and fatty acid composition of intracellular lipids were measured by GC analysis according to the previous method. [ 17 ] For GC analysis, we replaced n ‐hexane with n ‐heptane to improve the recovery efficiency for very‐long chain fatty acid methyl eaters (FAMEs).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Application of adaptive laboratory evolution to improve the tolerance of Rhodotorula strain to methanol in crude glycerol and development of an effective method for cell lysis

Wei,

Wang,

Fan

et al. 2023

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

Rhodotorula toruloides can utilize crude glycerol as the low‐cost carbon source for lipid production, but its growth is subjected to inhibition by methanol in crude glycerol. Here, transcriptome profiling demonstrated that 1004 genes were significantly regulated in the strain R. toruloides TO2 under methanol stress. Methanol impaired the function of membrane transport and subsequently weakened the utilization of glycerol, activities of the primary metabolism and functions of nucleus and ribosome. Afterwards the tolerance of TO2 to methanol was improved by using two‐round adaptive laboratory evolution (ALE). The final strain M2‐ale had tolerance up to 3.5% of methanol. 1H NMR‐based metabolome analysis indicated that ALE not only improved the tolerance of M2‐ale to methanol but also tuned the carbon flux towards the biosynthesis of glycerolipid‐related metabolites. The biomass and lipid titer of M2‐ale reached 14.63 ± 0.45 g L−1 and 7.06 ± 0.44 g L−1 at 96 h in the crude glycerol medium, which increased up to 17.69% and 31.39%, respectively, comparing with TO2. Afterwards, an effective method for cell lysis was developed by combining sonication and enzymatic hydrolysis (So‐EnH). The lytic effect of So‐EnH was validated by using confocal imaging and flow cytometry. At last, lipid recovery rate reached 95.4 ± 2.7% at the optimized condition.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Transcriptome analysis has been used in R. toruloides studies to find functional genes and promoters [ 27,28 ] and to elucidate the effect of carbon sources on the fermentation performance. [ 22,29 ] Here transcriptome analysis explained the inhibitory effect of methanol on the growth of TO2. Methanol impaired the function of membrane transport especially the activity of glycerol transport.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Application of adaptive laboratory evolution to improve the tolerance of Rhodotorula strain to methanol in crude glycerol and development of an effective method for cell lysis

Wei,

Wang,

Fan

et al. 2023

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Zhu et al (2012) reported proteomic and transcriptomic data of R. toruloides NP11 and suggested that lipid accumulation under nitrogen-limited conditions correlates with the induction of nitrogenous compound recycling, lipogenesis, autophagy, and macromolecule metabolism [8]. Recently, mutant R. toruloides showed increased lipid production due to downregulation of mannitol biosynthesis using glycerol as carbon source [38]. In another study, LC-MS/MSbased metabolomic analysis of Y. lipolytica was performed for studying the impact of nitrogen-limiting conditions which revealed that pyrimidine and purine metabolism were the most highly affected biological pathways [39].…”

Section: Lc-ms/ms Analysismentioning

confidence: 99%

Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in Rhodosporidium toruloides

Deeba

Kumar

Rajacharya

et al. 2021

JoF

View full text Add to dashboard Cite

Oleaginous yeast Rhodosporidium toruloides has great biotechnological potential and scientific interest, yet the molecular rationale of its cellular behavior to carbon and nitrogen ratios with concurrent lipid agglomeration remains elusive. Here, metabolomics adaptations of the R. toruloides in response to varying glucose and nitrogen concentrations have been investigated. In preliminary screening we found that 5% glucose (w/v) was optimal for further analysis in Rhodosporidium toruloides 3641. Hereafter, the effect of complementation to increase lipid agglomeration was evaluated with different nitrogen sources and their concentration. The results obtained illustrated that the biomass (13 g/L) and lipid (9.1 g/L) production were maximum on 5% (w/v) glucose and 0.12% (NH4)2SO4. Furthermore, to shed lights on lipid accumulation induced by nitrogen-limitation, we performed metabolomic analysis of the oleaginous yeast R. toruloides 3641. Significant changes were observed in metabolite concentrations by qualitative metabolomics through gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), which were mapped onto the governing metabolic pathways. Notable finding in this strain concerns glycerol and CDP-DAG metabolism wherein reduced production of glycerol and phospholipids induced a bypass leading to enhanced de-novo triacylglyceride synthesis. Collectively, our findings help in understanding the central carbon metabolism of R. toruloides which may assist in developing rationale metabolic models and engineering efforts in this organism.

show abstract

Attenuating the triacylglycerol catabolism enhanced lipid production of Rhodotorula strain U13N3

Song

Meng

et al. 2023

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Transcriptomic and metabolomic profiling of a Rhodotorula color mutant to improve its lipid productivity in fed-batch fermentation

Cited by 7 publications

References 33 publications

Application of adaptive laboratory evolution to improve the tolerance of Rhodotorula strain to methanol in crude glycerol and development of an effective method for cell lysis

Application of adaptive laboratory evolution to improve the tolerance of Rhodotorula strain to methanol in crude glycerol and development of an effective method for cell lysis

Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in Rhodosporidium toruloides

Attenuating the triacylglycerol catabolism enhanced lipid production of Rhodotorula strain U13N3

Contact Info

Product

Resources

About