2021
DOI: 10.1016/j.envexpbot.2020.104292
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Transcriptomic and physiological comparsion of the short-term responses of two Oryza sativa L. varieties to cadmium

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Cited by 19 publications
(6 citation statements)
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“…To verify the reliability of the transcriptome results, 8 DEGs co-expressed in B116-CK, B116-T, B144-CK, and B144-T were selected, and the primers were designed using SnapGene 3.2.1 (the sequences are listed in Supplementary Table S1 ) and were synthesized by Tsingke Biotechnology Co., Ltd. (Beijing, China) before then being synthesized using the ChamQ Universal SYBR ® qPCR Master Mix Q711 (Vazyme Biotech Co., Ltd., Nanjing, China). The RNA used for quantitative RT-PCR was the same as that used to construct the cDNA library, and the related genes were normalized to Actin transcript levels by the 2 −ΔΔCt method [ 37 ].…”
Section: Methodsmentioning
confidence: 99%
“…To verify the reliability of the transcriptome results, 8 DEGs co-expressed in B116-CK, B116-T, B144-CK, and B144-T were selected, and the primers were designed using SnapGene 3.2.1 (the sequences are listed in Supplementary Table S1 ) and were synthesized by Tsingke Biotechnology Co., Ltd. (Beijing, China) before then being synthesized using the ChamQ Universal SYBR ® qPCR Master Mix Q711 (Vazyme Biotech Co., Ltd., Nanjing, China). The RNA used for quantitative RT-PCR was the same as that used to construct the cDNA library, and the related genes were normalized to Actin transcript levels by the 2 −ΔΔCt method [ 37 ].…”
Section: Methodsmentioning
confidence: 99%
“…We slightly modified the method of Chen et al (2021) [ 77 ]: First, the roots, stems, and leaves were washed with 20 mM EDTA-Na 2 solution for 30 min, and then with deionized water three to five times. The washed samples were dried at 105 °C for 25 min, and then at 80 °C until they were completely dry.…”
Section: Methodsmentioning
confidence: 99%
“…The culture medium was replaced every 3 d. Subsequently, the plants were transferred into two hydroponic boxes (l × w × h: 37.5 cm × 25.0 cm × 13.5 cm; 12 seedlings per box) with 10 L 1/2 modified Hoagland nutrient solution (renewed every 3 d). One month later, seedlings in one box were treated with 50 μM Cd 2+ (via CdCl 2 •2.5H 2 O), an appropriate Cd concentration that can effectively trigger changes in plant gene expression in a short time ( Chen et al., 2021 ; Wang et al., 2022 ), whereas those in the other remained untreated as controls.…”
Section: Methodsmentioning
confidence: 99%