Transcriptomic basis of functional difference and coordination between seeds and the silique wall of Brassica napus during the seed-filling stage

Liu, Han; Yang, Qingyong; Fan, Chuchuan; Zhao, Xiaoqin; Wang, Xuemin; Zhou, Yongming

doi:10.1016/j.plantsci.2015.01.015

Cited by 9 publications

(10 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). Two genes [BnaC03g45080D (TIP41) and BnaA09g14410D (PP2A-1)] were used as internal controls (Liu et al, 2015a;Wu et al, 2016). Primers were designed based on the reference genome sequences using Primer Premier 5.0 and are listed in Table S2.…”

Section: Rna Extraction and Qrt-pcr Analysismentioning

confidence: 99%

A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus

Zhao

Cai

et al. 2019

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

Summary One of the most important goals in the breeding of oilseed crops, including Brassica napus, is to improve the quality of edible vegetable oil, which is mainly determined by the seed fatty acid composition, particularly the C18:1 content. Previous studies have indicated that the C18:1 content is a polygenic trait, and no stable quantitative trait loci (QTLs) except for FAD2 have been reported. By performing a GWAS using 375 low erucic acid B. napus accessions genotyped with the Brassica 60K SNP array and constructing a high‐density SNP‐based genetic map of a 150 DH population, we identified a novel QTL on the A9 chromosome. The novel locus could explain 11.25%, 5.72% and 6.29% of phenotypic variation during three consecutive seasons and increased the C18:1 content by approximately 3%–5%. By fine mapping and gene expression analysis, we found three potential candidate genes and verified the fatty acids in a homologous gene mutant of Arabidopsis. A metal ion‐binding protein was found to be the most likely candidate gene in the region. Thus, the C18:1 content can be further increased to about 80% with this novel locus together with FAD2 mutant allele without compromise of agronomic performance. A closely linked marker, BnA129, for this novel QTL (OLEA9) was developed so that we can effectively identify materials with high C18:1 content at an early growth stage by marker‐assisted selection. Our results may also provide new insight for understanding the complex genetic mechanism of fatty acid metabolism.

show abstract

Section: Rna Extraction and Qrt-pcr Analysismentioning

confidence: 99%

A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus

Zhao

Cai

et al. 2019

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…In OSR, silique is a composite organ that comprises seeds and silique wall (SW) [13]. SW protects developing seeds inside, and provides the source to sink transport links to accumulate storage reserves in seeds and provide nutrients.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Developing Seeds and Silique Wall Reveals Dynamic Transcription Networks for Effective Oil Production in Brassica napus L.

Shahid

Cai

Zhang

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Vegetable oil is an essential constituent of the human diet and renewable raw material for industrial applications. Enhancing oil production by increasing seed oil content in oil crops is the most viable, environmentally friendly, and sustainable approach to meet the continuous demand for the supply of vegetable oil globally. An in-depth understanding of the gene networks involved in oil biosynthesis during seed development is a prerequisite for breeding high-oil-content varieties. Rapeseed (Brassica napus) is one of the most important oil crops cultivated on multiple continents, contributing more than 15% of the world’s edible oil supply. To understand the phasic nature of oil biosynthesis and the dynamic regulation of key pathways for effective oil accumulation in B. napus, comparative transcriptomic profiling was performed with developing seeds and silique wall (SW) tissues of two contrasting inbred lines with ~13% difference in seed oil content. Differentially expressed genes (DEGs) between high- and low-oil content lines were identified across six key developmental stages, and gene enrichment analysis revealed that genes related to photosynthesis, metabolism, carbohydrates, lipids, phytohormones, transporters, and triacylglycerol and fatty acid synthesis tended to be upregulated in the high-oil-content line. Differentially regulated DEG patterns were revealed for the control of metabolite and photosynthate production in SW and oil biosynthesis and accumulation in seeds. Quantitative assays of carbohydrates and hormones during seed development together with gene expression profiling of relevant pathways revealed their fundamental effects on effective oil accumulation. Our results thus provide insights into the molecular basis of high seed oil content (SOC) and a new direction for developing high-SOC rapeseed and other oil crops.

show abstract

“…In Brassica napus , 30–40 days after flowering was the fastest-growing period for seed development, which determined mainly yield formation ( Basnet et al, 2013 ; Liu et al, 2015a ). To understand the possible mechanisms of the molecular regulation of higher yields in the special XRD eco-environment, we conducted a comparative transcriptomic study of rapeseed at 30 and 40 days after flowering (DAF) in two different areas (XRD and XN) with three biological replicates.…”

Section: Resultsmentioning

confidence: 99%

“…Seed-filling duration is the morphology and physiological metabolism of rapeseed that will change dramatically in the process of grain filling, involving the supply of various components and precursors from the leaves into developing seeds, and diverse biochemical processes take place to synthesize carbohydrates, proteins, and lipids ( Triboi et al, 2003 ; Barnabas et al, 2008 ; Awasthi et al, 2014 ). The yield and quality of grains are determined by the amount of organic matter synthesized or stored and the direction of transport, especially the photosynthate transport from the pod to seeds ( Diepenbrock, 2000 ; Hajduch et al, 2006 ; Liu et al, 2015a ). The production and transportation of photosynthetic assimilation are affected not only by the characteristics of the crops themselves but also by biotic or abiotic factors in the environment.…”

Section: Discussionmentioning

confidence: 99%

“…Seed development is an important stage in determining the yield of rapeseed ( Hay and Schwender, 2011 ; Basnet et al, 2013 ; Borisjuk et al, 2013 ; Liu et al, 2015a ; Gupta et al, 2017 ). A previous study suggested that cellular activities during seed filling in B. napus begin with sugar mobilization, followed by sequential surges in amino acid, lipid, and storage protein synthesis ( Hajduch et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptomic analysis of rapeseed (Brassica napus. L.) seed development in Xiangride, Qinghai Plateau, reveals how its special eco-environment results in high yield in high-altitude areas

et al. 2022

View full text Add to dashboard Cite

As one of the most important oil crops, rapeseed (Brassica napus) is cultivated worldwide to produce vegetable oil, animal feed, and biodiesel. As the population grows and the need for renewable energy increases, the breeding and cultivation of high-yield rapeseed varieties have become top priorities. The formation of a high rapeseed yield is so complex because it is influenced not only by genetic mechanisms but also by many environmental conditions, such as climatic conditions and different farming practices. Interestingly, many high-yield areas are located in special eco-environments, for example, in the high-altitude Xiangride area of the Qinghai Plateau. However, the molecular mechanisms underlying the formation of high yields in such a special eco-environment area remain largely unknown. Here, we conducted field yield analysis and transcriptome analysis in the Xiangride area. Compared with the yield and environmental factors in the Xinning area (a low-yielding area), we found that the relatively longer daylight length is the key to high rapeseed yield in the Xiangride area, which leads up to a 52.1% increase in rapeseed yield, especially the increase in thousand seed weight and silique number (SN). Combined with transcriptome H-cluster analysis and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analyses, we can assume that the grain development of rapeseed in the Xiangride area is ahead of schedule and lasts for a long time, leading to the high-yield results in the Xiangride area, confirmed by the expression analysis by quantitative real-time polymerase chain reaction (qRT-PCR) of yield-related genes. Our results provide valuable information for further exploring the molecular mechanism underlying high yield in special ecological environments and provide a helpful reference for studying seed development characteristics in special-producing regions for Brassica napus.

show abstract

Transcriptomic basis of functional difference and coordination between seeds and the silique wall of Brassica napus during the seed-filling stage

Cited by 9 publications

References 79 publications

A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus

A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus

Comparative Transcriptome Analysis of Developing Seeds and Silique Wall Reveals Dynamic Transcription Networks for Effective Oil Production in Brassica napus L.

Transcriptomic analysis of rapeseed (Brassica napus. L.) seed development in Xiangride, Qinghai Plateau, reveals how its special eco-environment results in high yield in high-altitude areas

Contact Info

Product

Resources

About