Transcriptomic changes during tuber dormancy release process revealed by RNA sequencing in potato

Liu, Bailin; Zhang, Ning; Wen, Yan; Jin, Xin; Yang, Jie; Si, Hongyu; Wang, Di

doi:10.1016/j.jbiotec.2015.01.019

Cited by 39 publications

(39 citation statements)

References 81 publications

(121 reference statements)

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“…To investigate whether NO improves the tolerance to ABA, dormant tubers were sprayed with either DW (control), 100 µM ABA, 100 µM ABA + 40 µM SNP, or 100 µM ABA + 1 mM c-PTIO for 15 min. The bud eye tissues were sampled at 0, 10, 20, 30, 40, 50, and 60 days (Liu et al, 2015).…”

Section: Tuber Sprouting Testmentioning

confidence: 99%

“…Plant dormancy and subsequent germination are physiological processes of active adaptation to the environment. Potato tuber dormancy is defined as an inability to sprout under conditions that are favorable for sprouting (Liu et al, 2015). The dormancy and sprouting of potato tubers are very significant for potato cultivation, tuber production, and industrial processing (Aksenova et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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NO and ABA Interaction Regulates Tuber Dormancy and Sprouting in Potato

Wang

Zhao

et al. 2020

Front. Plant Sci.

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In plants, nitric oxide synthase (NOS)-like or nitrate reductase (NR) produces nitric oxide (NO), which is involved in releasing seed dormancy. However, its mechanism of effect in potato remains unclear. In this study, spraying 40 µM sodium nitroprusside (SNP), an exogenous NO donor, quickly broke tuber dormancy and efficiently promoted tuber sprouting, whereas 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3oxide (c-PTIO), an NO scavenger, repressed the influence of NO on tuber sprouting. Compared with the control (distilled water), SNP treatment led to a rapid increase in NO content after 6 h and a decreased abscisic acid (ABA) content at 12 and 24 h. c-PTIO treatment significantly inhibited increase of NO levels and increased ABA production. In addition, N G-nitro-L-arginine methyl ester, an NOS inhibitor, clearly inhibited the NOS-like activity, whereas tungstate, an NR inhibitor, inhibited the NR activity. Furthermore, NO promoted the expression of a gene involved in ABA catabolism (StCYP707A1, encoding ABA 8-hydroxylase) and inhibited the expression of a gene involved in ABA biosynthesis (StNCED1, encoding 9-cis-epoxycarotenoid dioxygenase), thereby decreasing the ABA content, disrupting the balance between ABA and gibberellin acid (GA), and ultimately inducing dormancy release and tuber sprouting. The results demonstrated that NOSlike or NR-generated NO controlled potato tuber dormancy release and sprouting via ABA metabolism and signaling in tuber buds.

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Section: Tuber Sprouting Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NO and ABA Interaction Regulates Tuber Dormancy and Sprouting in Potato

Wang

Zhao

et al. 2020

Front. Plant Sci.

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“…Liu et al identified 26,639 genes, including 5912 and 3885 differentially expressed genes from dormancy tuber (DT) vs. dormancy release tuber (DRT) and DRT vs. sprouting tuber (ST), respectively, using RNA-seq. Moreover, these authors showed that dormancy release was accompanied by stress response and redox regulation [ 12 ]. Isobaric tag for relative and absolute quantitation (iTRAQ) is considered one of the most robust methods of differential quantitative proteomics analysis [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Morphology, Transcription, and Proteomics Study Revealing the Key Molecular Mechanism of Camphor on the Potato Tuber Sprouting Effect

Xue

Deng

et al. 2017

IJMS

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Sprouting regulation in potato tubers is important for improving commercial value and producing new plants. Camphor shows flexible inhibition of tuber sprouting and prolongs the storage period of potato, but its underlying mechanism remains unknown. The results of the present study suggest that camphor inhibition caused bud growth deformities and necrosis, but after moving to more ventilated conditions, new sprouts grew from the bud eye of the tuber. Subsequently, the sucrose and fructose contents as well as polyphenol oxidase (PPO) activity were assessed after camphor inhibition. Transcription and proteomics data from dormancy (D), sprouting (S), camphor inhibition (C), and recovery sprouting (R) samples showed changes in the expression levels of approximately 4000 transcripts, and 700 proteins showed different abundances. KEGG (Kyoto encyclopaedia of genes and genomes) pathway analysis of the transcription levels indicated that phytohormone synthesis and signal transduction play important roles in tuber sprouting. Camphor inhibited these processes, particularly for gibberellic acid, brassinosteroids, and ethylene, leading to dysregulation of physiological processes such as cutin, suberine and wax biosynthesis, fatty acid elongation, phenylpropanoid biosynthesis, and starch and sucrose metabolism, resulting in bud necrosis and prolonged storage periods. The KEGG pathway correlation between transcripts and proteins revealed that terpenoid backbone biosynthesis and plant–pathogen interaction pathways showed significant differences in D vs. S samples, but 13 pathways were remarkably different in the D vs. C groups, as camphor inhibition significantly increased both the transcription levels and protein abundance of pathogenesis-related protein PR-10a (or STH-2), the pathogenesis-related P2-like precursor protein, and the kirola-like protein as compared to sprouting. In recovery sprouting, these genes and proteins were decreased at both the transcriptional level and in protein abundance. It was important to find that the inhibitory effect of camphor on potato tuber sprout was reversible, revealing the action mechanism was similar to resistance to pathogen infection. The present study provides a theoretical basis for the application of camphor in prolonging seed potato storage.

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“…As of March, 2017, the original publication of the DM sequence has been cited more than 500 times, providing a framework for studies of gene families (Charfeddine et al 2015;Gao et al 2016;Ma et al 2016;Schreiber et al 2014;Seo et al 2016;Tang et al 2016;Van Harsselaar et al 2017), a scaffold for alignment of transcriptomic data (Campbell et al 2014;Gong et al 2015;Goyer et al 2015;Liu et al 2015;Morris et al 2014;Tang et al 2014) or a reference genome against which to discover genomic variation (Hardigan et al 2016), to cite just a few. As sequencing platforms improve, the DM assembly will likely be supplanted by that of a more robust commercial potato line.…”

Section: Resultsmentioning

confidence: 99%