Transcriptomic changes in bovine skeletal muscle cells after resveratrol treatment

Hao, Dan; Wang, Xiaogang; Wang, Xiao; Thomsen, Bo; Kadarmideen, Haja N.; Lan, Xianyong; Huang, Yongzhen; Chen, Hong

doi:10.1016/j.gene.2020.144849

Cited by 9 publications

(13 citation statements)

References 58 publications

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“…The molecular mechanisms triggered by R have been investigated in farm animal species both in vitro and in vivo. For instance, a whole transcriptome study conducted in bovine skeletal muscle cells has recently demonstrated how R significantly affects the mRNA expression of genes regulating cholesterol and fatty acid metabolism, cell cycle and proliferation [ 49 ]. In the same study, sirtuins (SIRTs), regulatory proteins known to protect from inflammation, suppress cell death and activate antioxidant genes [ 50 , 51 , 52 ], appeared to be differentially regulated by R to a significant extent.…”

Section: Introductionmentioning

confidence: 99%

Discovering the Protective Effects of Resveratrol on Aflatoxin B1-Induced Toxicity: A Whole Transcriptomic Study in a Bovine Hepatocyte Cell Line

et al. 2021

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Aflatoxin B1 (AFB1) is a natural feed and food contaminant classified as a group I carcinogen for humans. In the dairy industry, AFB1 and its derivative, AFM1, are of concern for the related economic losses and their possible presence in milk and dairy food products. Among its toxic effects, AFB1 can cause oxidative stress. Thus, dietary supplementation with natural antioxidants has been considered among the strategies to mitigate AFB1 presence and its toxicity. Here, the protective role of resveratrol (R) has been investigated in a foetal bovine hepatocyte cell line (BFH12) exposed to AFB1, by measuring cytotoxicity, transcriptional changes (RNA sequencing), and targeted post-transcriptional modifications (lipid peroxidation, NQO1 and CYP3A enzymatic activity). Resveratrol reversed the AFB1-dependent cytotoxicity. As for gene expression, when administered alone, R induced neglectable changes in BFH12 cells. Conversely, when comparing AFB1-exposed cells with those co-incubated with R+AFB1, greater transcriptional variations were observed (i.e., 840 DEGs). Functional analyses revealed that several significant genes were involved in lipid biosynthesis, response to external stimulus, drug metabolism, and inflammatory response. As for NQO1 and CYP3A activities and lipid peroxidation, R significantly reverted variations induced by AFB1, mostly corroborating and/or completing transcriptional data. Outcomes of the present study provide new knowledge about key molecular mechanisms involved in R antioxidant-mediated protection against AFB1 toxicity.

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Section: Introductionmentioning

confidence: 99%

Discovering the Protective Effects of Resveratrol on Aflatoxin B1-Induced Toxicity: A Whole Transcriptomic Study in a Bovine Hepatocyte Cell Line

et al. 2021

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“…The cultured primary myoblasts from the fetal beef longissimus dorsi muscle, the transcriptome sequencing datasets after quality control and alignment, and the differential expression analysis results were achieved from our previous studies (31,32).…”

Section: Primary Bovine Myoblast Transcriptome Sequencing and Differe...mentioning

confidence: 99%

“…RNA for each cell sample was isolated for mRNA sequencing to generate 125 or 150-bp paired-end reads and for miRNA sequencing to generate 50-bp single-end reads on an Illumina Hiseq platform (Illumina, USA). We removed the unqualified reads by quality control to achieve clean reads by in-house perl scripts that were used in our previous studies (31,32). Then, the miRNA tags were mapped to the reference genome of Bos taurus (UMD_3.1.1/bosTau8) by Bowtie software (version 0.12.9) (33).…”

Section: Primary Bovine Myoblast Transcriptome Sequencing and Differe...mentioning

confidence: 99%

“…From our previous study (31,32), a total of 3,856 DE mRNAs were identified from 18,329 mRNAs based on the threshold of adjusted P-value <0.05; meanwhile, 93 DE miRNAs were also identified from 650 miRNAs based on the same thresholds (Table 1). The details of log 2 FC, P-value, adjusted P-value, and DE mRNAs with FPKM and miRNAs with TPM of each sample are listed in the Supplemental File 1.…”

Section: Differentially Expressed Mrnas and Mirnas Between The Rsv Tr...mentioning

confidence: 99%

“…Our previous study focused on transcriptomic changes in bovine skeletal muscle cells after RSV treatment and was conducted to identify the differentially expressed (DE) genes and microRNAs (miRNAs) (31,32); therefore, this study mainly focuses on the combined co-expressed transcriptomes, i.e., messenger RNA (mRNA) and miRNA studies for bovine muscle in response to treatment with RSV, which aims to investigate the roles of RSV in inducing miRNA for the better understanding, to identify the characteristics of DE miRNAs in the key co-expressed module in regulation of mRNAs, and to reveal new candidate regulatory miRNAs and genes underlying the beef quality traits.…”

Section: Introductionmentioning

confidence: 99%

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Integrated Analysis of mRNA and MicroRNA Co-expressed Network for the Differentiation of Bovine Skeletal Muscle Cells After Polyphenol Resveratrol Treatment

Hao

Wang

et al. 2021

Front. Vet. Sci.

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Resveratrol (RSV) has been confirmed to benefit human health. Resveratrol supplemented in the feeds of animals improved pork, chicken, and duck meat qualities. In this study, we identified differentially expressed (DE) messenger RNAs (mRNAs) (n = 3,856) and microRNAs (miRNAs) (n = 93) for the weighted gene co-expression network analysis (WGCNA) to investigate the co-expressed DE mRNAs and DE miRNAs in the primary bovine myoblasts after RSV treatment. The mRNA results indicated that RSV treatments had high correlations with turquoise module (0.91, P-value = 0.01) and blue module (0.93, P-value < 0.01), while only the turquoise module (0.96, P-value < 0.01) was highly correlated with the treatment status using miRNA data. After biological enrichment analysis, the 2,579 DE genes in the turquoise module were significantly enriched in the Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The top two GO terms were actin filament-based process (GO:0030029) and actin cytoskeleton organization (GO:0030036). The top two KEGG pathways were regulation of actin cytoskeleton (bta04810) and tight junction (bta04530). Then, we constructed the DE mRNA co-expression and DE miRNA co-expression networks in the turquoise module and the mRNA–miRNA targeting networks based on their co-expressions in the key module. In summary, the RSV-induced miRNAs participated in the co-expression networks that could affect mRNA expressions to regulate the primary myoblast differentiation. Our study provided a better understanding of the roles of RSV in inducing miRNA and of the characteristics of DE miRNAs in the key co-expressed module in regulation of mRNAs and revealed new candidate regulatory miRNAs and genes for the beef quality traits.

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Resveratrol-Based Fluorinated Materials with High Thermostability and Good Dielectric Properties at High Frequency

Fang

Tao

Wang

et al. 2020

ACS Sustainable Chem. Eng.

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Two resveratrol-based monomers with thermo-cross-linkable trifluorovinyl-ether groups (−OCFCF2) have been designed and synthesized. After treating at a temperature above 140 °C, the monomers were converted into cross-linked networks, which display the average dielectric constants (D k) of 2.45–2.52 and the average dielectric loss (D f) of 2.2 × 10–3 to 2.6 × 10–3 at a high frequency of 5 GHz. Such data are much better than these of the resveratrol-based epoxy resin (D k of above 3.5 and D f of near 2.6 × 10–2) and the commercial bisphenol A epoxy resins (D k of above 3.7). In addition, two cured monomers exhibit high thermostability with a 5% weight loss temperature (T 5d) of more than 450 °C. They also display low water uptake of below 0.08% when immersed in boiling water for 3 days. These data indicate that the resveratrol-based monomers in this contribution are suitable as the encapsulation resins for the application in the microelectronic industry.

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Transcriptomic changes in bovine skeletal muscle cells after resveratrol treatment

Cited by 9 publications

References 58 publications

Discovering the Protective Effects of Resveratrol on Aflatoxin B1-Induced Toxicity: A Whole Transcriptomic Study in a Bovine Hepatocyte Cell Line

Discovering the Protective Effects of Resveratrol on Aflatoxin B1-Induced Toxicity: A Whole Transcriptomic Study in a Bovine Hepatocyte Cell Line

Integrated Analysis of mRNA and MicroRNA Co-expressed Network for the Differentiation of Bovine Skeletal Muscle Cells After Polyphenol Resveratrol Treatment

Resveratrol-Based Fluorinated Materials with High Thermostability and Good Dielectric Properties at High Frequency

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