Transcriptomic Changes in Hot Spring Frog Tadpoles (Buergeria otai) in Response to Heat Stress

Komaki, Shohei; Matsunami, Masatoshi; Lin, Jung‐Yaw; Lee, Ko Huan; Lin, Yen-Po; Yu, Liang; Lin, Si Min; Igawa, Takeshi

doi:10.3389/fevo.2021.706887

Cited by 3 publications

(4 citation statements)

References 46 publications

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“…The reverse transcription reaction was performed with extracted total RNA as a template and oligo-dT primers to synthesize complementary DNA using the ReverTra Ace® (Toyobo, Osaka, Japan), according to the manufacturer’s instructions. The PCR primers were designed according to the nucleotide sequences of TRPV1 or TRPA1 of B. japonica obtained by RNA sequencing analysis ( Komaki et al 2021 ). For TRPV1, the first round of PCR was performed using the reverse-transcribed products as the template with forward (5′-CATCAGAAGGAATCATTTTAAGACTAGC-3′) and reverse (5′-TGTTTGTTATCCAACTTACCAGTGTC-3′) primers.…”

Section: Methodsmentioning

confidence: 99%

Evolutionary Tuning of Transient Receptor Potential Ankyrin 1 Underlies the Variation in Heat Avoidance Behaviors among Frog Species Inhabiting Diverse Thermal Niches

Saito

Igawa

et al. 2022

Molecular Biology and Evolution

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Environmental temperature is a critical factor for all forms of life, and thermal tolerance defines the habitats utilized by a species. Moreover, the evolutionary tuning of thermal perception can also play a key role in habitat selection. Yet, the relative importance of thermal tolerance and perception in environmental adaptation remains poorly understood. Thermal conditions experienced by anuran tadpoles differ among species due to the variation in breeding seasons and water environments selected by parental frogs. In the present study, heat tolerance and avoidance temperatures were compared in tadpoles from five anuran species that spatially and temporally inhabit different thermal niches. These two parameters were positively correlated with each other and were consistent with the thermal conditions of habitats. The species difference in avoidance temperature was 2.6 times larger than that in heat tolerance, suggesting the importance of heat avoidance responses in habitat selection. In addition, the avoidance temperature increased after warm acclimation, especially in the species frequently exposed to heat in their habitats. Characterization of the heat-sensing TRPA1 ion channel revealed an amphibian-specific alternatively spliced variant containing a single valine insertion relative to the canonical alternative spliced variant of TRPA1, and this novel variant altered the response to thermal stimuli. The two alternatively spliced variants of TRPA1 exhibited different thermal responses in a species-specific manner, which are likely to be associated with a difference in avoidance temperatures among species. Together, our findings suggest that the functional change in TRPA1 plays a crucial role in thermal adaptation processes.

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Section: Methodsmentioning

confidence: 99%

Evolutionary Tuning of Transient Receptor Potential Ankyrin 1 Underlies the Variation in Heat Avoidance Behaviors among Frog Species Inhabiting Diverse Thermal Niches

Saito

Igawa

et al. 2022

Molecular Biology and Evolution

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“…These are unexpected results. HSP genes were likewise downregulated in E. tynerensis (Oklahoma salamander) [ 35 ] and B. otai tadpoles [ 23 ], but upregulated in Quasipaa spinosa [ 64 ] under heat stress. Heat shock proteins are induced by a variety of stressors, despite their name referring to their reaction to heat stress.…”

Section: Discussionmentioning

confidence: 99%

“…Comparative transcriptome analysis of three acclimatized groups of Quasipaa boulengeri tadpoles found key differentially expressed genes which coincided with phenotype and hormone level and offered a suitable rearing temperature [ 22 ]. Transcriptomic profiles and protein-protein interaction analysis of heat-treated Buergeria otai tadpoles exhibit global suppression of DNA transcription and mRNA translation that ensure them to survive high temperatures [ 23 ]. Similarly, through transcriptome analysis, it was found that genes involved in secretion and defensive function are highly expressed in the skin of Rana chensinensis [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Potential Effects of High Temperature and Heat Wave on Nanorana pleskei Based on Transcriptomic Analysis

Zhang

Niu

et al. 2023

CIMB

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In the context of climate change, understanding how indigenous amphibians of the Qinghai-Tibet plateau react to stresses and their coping mechanisms could be crucial for predicting their fate and successful conservation. A liver transcriptome for Nanorana pleskei was constructed using high-throughput RNA sequencing, and its gene expression was compared with frogs acclimated under either room temperature or high temperature and also heat wave exposed ones. A total of 126,465 unigenes were produced, with 66,924 (52.92%) of them being annotated. Up to 694 genes were found to be differently regulated as a result of abnormal temperature acclimatization. Notably, genes belonging to the heat shock protein (HSP) family were down-regulated in both treated groups. Long-term exposure to high-temperature stress may impair the metabolic rate of the frog and trigger the body to maintain a hypometabolic state in an effort to survive challenging times. During heat waves, unlike the high-temperature group, mitochondrial function was not impaired, and the energy supply was largely normal to support the highly energy-consuming metabolic processes. Genes were more transcriptionally suppressed when treated with high temperatures than heat waves, and the body stayed in low-energy states for combating these long-term adverse environments to survive. It might be strategic to preserve initiation to executive protein activity under heat wave stress. Under both stress conditions, compromising the protection of HSP and sluggish steroid activity occurred in frogs. Frogs were more affected by high temperatures than by heat waves.

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Long-Term Heat Tolerance and Accelerated Metamorphosis: Hot Spring Adaptations of Buergeria japonica

Priambodo,

Shiraga,

Harada

et al. 2024

Zoological Science

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Transcriptomic Changes in Hot Spring Frog Tadpoles (Buergeria otai) in Response to Heat Stress

Cited by 3 publications

References 46 publications

Evolutionary Tuning of Transient Receptor Potential Ankyrin 1 Underlies the Variation in Heat Avoidance Behaviors among Frog Species Inhabiting Diverse Thermal Niches

Evolutionary Tuning of Transient Receptor Potential Ankyrin 1 Underlies the Variation in Heat Avoidance Behaviors among Frog Species Inhabiting Diverse Thermal Niches

Potential Effects of High Temperature and Heat Wave on Nanorana pleskei Based on Transcriptomic Analysis

Long-Term Heat Tolerance and Accelerated Metamorphosis: Hot Spring Adaptations of Buergeria japonica

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