Transcriptomic Changes in Young Japanese Males After Exposure to Acute Hypobaric Hypoxia

Yasukochi, Yoshiki; Shin, Sora; Wakabayashi, Hisao; Maeda, Takafumi

doi:10.3389/fgene.2020.559074

Cited by 11 publications

(10 citation statements)

References 61 publications

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“…1 b) indicated that the expression vectors of samples from the same individuals tended to be clustered, irrespective of experimental conditions. This result is similar to that of our previous study on transcriptomic changes in response to hypobaric hypoxic exposure [ 40 ]. The transcriptomic profiles of 50 genes with the highest variance across samples also showed the clustering of vectors from each individual (Fig.…”

Section: Resultssupporting

confidence: 92%

“…Therefore, we identified the 13 genes as moderate DEGs. Differential expression analysis for the hypobaric hypoxic experiment in our previous study identified 30 upregulated DEGs [ 40 ], whereas the present study on cold exposure detected a single upregulated DEG (i.e., CTSL ). The sample size of the previous study (three individuals) was smaller than that of the present study (six individuals); additionally, we confirmed no significant difference in the gene expression levels under no-stimulus conditions [ 40 ].…”

Section: Resultscontrasting

confidence: 62%

“…Differential expression analysis for the hypobaric hypoxic experiment in our previous study identified 30 upregulated DEGs [ 40 ], whereas the present study on cold exposure detected a single upregulated DEG (i.e., CTSL ). The sample size of the previous study (three individuals) was smaller than that of the present study (six individuals); additionally, we confirmed no significant difference in the gene expression levels under no-stimulus conditions [ 40 ]. Consequently, the small number of the DEGs observed in the present study is unlikely due to the samples size and might be attributed to the possibilities described above (i.e., mild cold stress, short-term cold exposure, blunted transcriptional response in saliva to cold stress, or seasonal acclimatization).…”

Section: Resultscontrasting

confidence: 62%

“…We conducted principal component analysis (PCA) to compare transcriptomic similarity among samples. The detailed methods mentioned above have been described previously [ 40 ]. The molecular function of DEG was predicted using the information on the AmiGO 2 ( http://amigo.geneontology.org/amigo , [ 41 ]) and Kyoto Encyclopedia of Genes and Genomes (KEGG; https://www.genome.jp/kegg/ ) databases.…”

Section: Methodsmentioning

confidence: 99%

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Upregulation of cathepsin L gene under mild cold conditions in young Japanese male adults

Yasukochi

Shin

Wakabayashi

et al. 2021

J Physiol Anthropol

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Background Physiological thermoregulatory systems in humans have been a key factor for adaptation to local environments after their exodus from Africa, particularly, to cold environments outside Africa. Recent studies using high-throughput sequencing have identified various genes responsible for cold adaptation. However, the molecular mechanisms underlying initial thermoregulation in response to acute cold exposure remain unclear. Therefore, we investigated transcriptional profiles of six young Japanese male adults exposed to acute cold stress. Methods In a climatic chamber, the air temperature was maintained at 28°C for 65 min and was then gradually decreased to 19°C for 70 min. Saliva samples were obtained from the subjects at 28°C before and after 19°C cold exposure and were used for RNA sequencing. Results In the cold exposure experiment, expression levels of 14 genes were significantly changed [false discovery rate (FDR) < 0.05] although the degree of transcriptional changes was not high due to experimental conditions or blunted transcriptional reaction in saliva to cold stress. As a result, differential gene expression analyses detected the cathepsin L (CTSL) gene to be significantly upregulated, with FDR < 0.05 and log2 fold change value > 1; thus, this gene was identified as a differentially expressed gene. Given that the cathepsin L protein is related to invasion of the novel coronavirus (SARS-CoV-2), mild cold stress might alter the susceptibility to coronavirus disease-19 in humans. The gene ontology enrichment analysis for 14 genes with FDR < 0.05 suggested that immune-related molecules could be activated by mild cold stress. Conclusions The results obtained from this study indicate that CTSL expression levels can be altered by acute mild cold stress.

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Section: Resultssupporting

confidence: 92%

Section: Resultscontrasting

confidence: 62%

Section: Resultscontrasting

confidence: 62%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Upregulation of cathepsin L gene under mild cold conditions in young Japanese male adults

Yasukochi

Shin

Wakabayashi

et al. 2021

J Physiol Anthropol

Self Cite

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“…Given that the HIF pathway regulates the immune and inflammatory responses, the finding of this study may contribute to the altered immune cell activity through HIF-related pathways affected by exposure to hypobaric hypoxia [ 23 ]. The connection between hypoxia and inflammation has become evident, as HIF also confers responses to immune stresses [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of 30 and 60 Min of Hypobaric Hypoxia in Simulated Altitude of 15,000 ft on Human Proteome Profile

Schmitz

Johnson

Liebold

et al. 2022

IJMS

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The human body reacts to hypobaric hypoxia, e.g., during a stay at high altitude, with several mechanisms of adaption. Even short-time exposition to hypobaric hypoxia leads to complex adaptions. Proteomics facilitates the possibility to detect changes in metabolism due to changes in proteins. The present study aims to identify time-dependent changes in protein expression due to hypobaric hypoxia for 30 and 60 min at a simulated altitude of 15,000 ft. N = 80 male subjects were randomized and assigned into four different groups: 40 subjects to ground control for 30 (GC30) and 60 min (GC60) and 40 subjects to 15,000 ft for 30 (HH30) and 60 min (HH60). Subjects in HH30 and HH60 were exposed to hypobaric hypoxia in a pressure chamber (total pressure: 572 hPa) equivalent to 15,000 ft for 30 vs. 60 min, respectively. Drawn blood was centrifuged and plasma frozen (−80 °C) until proteomic analysis. After separation of high abundant proteins, protein expression was analyzed by 2-DIGE and MALDI-TOF. To visualize the connected signaling cascade, a bio-informatical network analysis was performed. The present study was approved by the ethical committee of the University of Cologne, Germany. The study registry number is NCT03823677. In comparing HH30 to GC30, a total of seven protein spots had a doubled expression, and 22 spots had decreased gene expression. In a comparison of HH60 to GC60, a total of 27 protein spots were significantly higher expressed. HH60, as compared to GC30, revealed that a total of 37 spots had doubled expression. Vice versa, 12 spots were detected, which were higher expressed in GC30 vs. HH60. In comparison to GC, HH60 had distinct differences in the number of differential protein spots (noticeably more proteins due to longer exposure to hypoxia). There are indicators that changes in proteins are dependent on the length of hypobaric hypoxia. Some proteins associated with hemostasis were differentially expressed in the 60 min comparison.

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The human blood transcriptome exhibits time-of-day-dependent response to hypoxia: Lessons from the highest city in the world

Manella

Ezagouri²,

Champigneulle³

et al. 2022

Cell Reports

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Transcriptomic Changes in Young Japanese Males After Exposure to Acute Hypobaric Hypoxia

Cited by 11 publications

References 61 publications

Upregulation of cathepsin L gene under mild cold conditions in young Japanese male adults

Upregulation of cathepsin L gene under mild cold conditions in young Japanese male adults

Influence of 30 and 60 Min of Hypobaric Hypoxia in Simulated Altitude of 15,000 ft on Human Proteome Profile

The human blood transcriptome exhibits time-of-day-dependent response to hypoxia: Lessons from the highest city in the world

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