Transcriptomic data for analyzing global gene expression patterns in Methicillin-resistance Staphylococcus aureus in response to spermine and oxacillin stress

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a rapidly emerging bacteria causing infection, which has developed resistance to most of the beta-lactam antibiotics because of newly acquired low-affinity penicillin binding protein (PBP2a), which can continue to build the cell wall when other PBPs are blocked by beta-lactams. Exogenous spermine exerts a dose dependent inhibition effect on the growth of E. coli, Salmonella enterica serovar and Staphylococcus aureus. We have selected an MRSA Mu50 derivative which harbors mutation on PBP2 gene (named as MuM) showing spermine resistance and which confers a complete abolishment of spermine-beta-lactam synergy. A transcriptomic profiling of MuM against Mu50 (wild type) without any treatment, MuM and Mu50 in response to high dose spermine and Mu50 in response to spermine-beta-lactam synergy is provided in this article. These comparisons will enhance our current understanding of mechanisms of spermine-beta-lactam synergy sensitization effects on MRSA.

Transcriptome Analysis of Halotolerant Staphylococcus saprophyticus Isolated from Korean Fermented Shrimp

“…Although a microorganism possesses genes for stress response, a specific gene may not be actively expressed as RNA unless cells are exposed to severe growth conditions. RNA sequencing (RNA-seq) is a useful technology to investigate the stress response mechanisms in various microorganisms [ 9 , 10 , 11 , 12 ]. For example, transcriptome analysis of Staphylococcus aureus revealed the increased cell adhesion and biofilm formation in response to low concentrations of ampicillin [ 13 ].…”