Transcriptomic features of trophoblast lineage cells derived from human induced pluripotent stem cells treated with BMP 4

Omics Approaches to Study Formation and Function of Human Placental Syncytiotrophoblast

“…Similar to hESCs, transcriptome analyses of human induced PSCs (hiPSCs) cultured in defined conditions (such as with BMP4 or in micromesh culture) provide evidence that these cells possess STB-like gene expression profiles (Chen et al, 2013; Xu et al, 2002;Sudheer et al, 2012;Chen et al, 2013;Wei et al, 2017;Tsuchida et al, 2020 Transcriptome analyses supported the notion that BMP4-treated hESCs/hPSCs differentiate into cells possessing trophoblast-like properties. BMP activation together with inhibition of both activin/nodal and FGF signaling stimulated pronounced differentiation into STB-like cells.…”

“…Wei et al, 2017;Li et al, 2019;Mischler et al, 2021). Tsuchida et al (2020) treated hiPSCs with BMP4 for 10 days, and cells positive for the pan-trophoblast marker KRT7 were purified and compared to undifferentiated hiPSCs by DNA microarray. Hierarchical clustering separated the two groups of cells into distinct clusters, with KRT7+ cells expressing markers representative of trophoblast lineages including STB.…”

“…Similar to hESCs, transcriptome analyses of human induced PSCs (hiPSCs) cultured in defined conditions (such as with BMP4 or in micromesh culture) provide evidence that these cells possess STB-like gene expression profiles ( Chen et al, 2013 ; Wei et al, 2017 ; Li et al, 2019 ; Mischler et al, 2021 ). Tsuchida et al (2020) treated hiPSCs with BMP4 for 10 days, and cells positive for the pan-trophoblast marker KRT7 were purified and compared to undifferentiated hiPSCs by DNA microarray. Hierarchical clustering separated the two groups of cells into distinct clusters, with KRT7+ cells expressing markers representative of trophoblast lineages including STB.…”

“…Hierarchical clustering separated the two groups of cells into distinct clusters, with KRT7+ cells expressing markers representative of trophoblast lineages including STB. Furthermore, XAGE2 and KCNQ2 , which were upregulated in KRT7+ cells, exhibited distinct expression patterns in human placenta in situ ( Tsuchida et al, 2020 ). Li et al (2020) used whole-genome sequencing (WGS) along with published transcriptomic and epigenomic datasets to identify 6 genomic variations associated with genes upregulated following BMP4-mediated differentiation of hPSCs.…”

Comparação de redes de regulação gênica da placenta em resposta à exposição ao estresse entre sexos

“…Para entender melhor o desenvolvimento e a atividade da placenta, diversos modelos de iPS humanos foram desenvolvidos com o objetivo de gerar células primitivas e maduras do trofoblasto, e quase exclusivamente, são baseados na ativação do gene BMP4 (Bone Morphogenetic Protein 4)(Tsuchida et al, 2020). Embora esses modelos sejam ampliamente usados, existe um debate sobre a semelhança entre as células diferenciadas por esses modelos, e citotrofoblastos in vivo.…”