Transcriptomic Identification of Wheat AP2/ERF Transcription Factors and Functional Characterization of TaERF-6-3A in Response to Drought and Salinity Stresses

Yang, Yuanzheng; Yu, Mengying; Zhang, Shuangxing; Song, Tianqi; Zhang, Mingfei; Zhou, Hongwei; Wang, Yukun; Xiang, Jun; Zhang, Xiaoke

doi:10.3390/ijms23063272

Cited by 31 publications

(18 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We collected the transcriptomic data in six plant species to verify our hypothesis on the relevance that ERF members of specific subgroup involved in particular abiotic stresses, such as salinity, cold and drought ( Figure 4 ). The heatmap was generated by the software TBtools ( Chen C. J. et al., 2020 ) with the 113 ERF genes transcriptome values of log2FoldChange (Log2FC) in six species under drought, cold, and salinity stress, respectively ( Shankar et al., 2016 ; Jin et al., 2017 ; Li et al., 2017 ; Sharma et al., 2018 ; Guan et al., 2019 ; Sharmin et al., 2020 ; Liu X. et al., 2021 ; Yu et al., 2022 ) ( Supplemental Table S5 ). Expression profiles of expressed genes are presented with gradient blue and red boxes, blue represents low expression and red represents high expression.…”

Section: Transcriptional Regulation Of Erfs In Response To Abiotic St...mentioning

confidence: 99%

“… Heatmap of differentially expressed ERF genes in response to abiotic stresses. The heatmap was constructed by TBtools ( Chen C. J. et al., 2020 ) with the 113 ERF genes transcriptome values of log2FoldChange (Log2FC) in six species ( Arabidopsis thaliana L., Glycine max L., Nicotiana tabacum L., Oryza sativa L., Triticum aestivum L., and Zea mays L.) under drought, cold, and salinity stress, respectively ( Shankar et al., 2016 ; Jin et al., 2017 ; Li et al., 2017 ; Sharma et al., 2018 ; Guan et al., 2019 ; Sharmin et al., 2020 ; Liu X. et al., 2021 ; Yu et al., 2022 ) ( Supplemental Table S5 ). …”

Section: Transcriptional Regulation Of Erfs In Response To Abiotic St...mentioning

confidence: 99%

See 1 more Smart Citation

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

Zhang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Ethylene Responsive Factor (ERF) subfamily comprise the largest number of proteins in the plant AP2/ERF superfamily, and have been most extensively studied on the biological functions. Members of this subfamily have been proven to regulate plant resistances to various abiotic stresses, such as drought, salinity, chilling and some other adversities. Under these stresses, ERFs are usually activated by mitogen-activated protein kinase induced phosphorylation or escape from ubiquitin-ligase enzymes, and then form complex with nucleic proteins before binding to cis-element in promoter regions of stress responsive genes. In this review, we will discuss the phylogenetic relationships among the ERF subfamily proteins, summarize molecular mechanism how the transcriptional activity of ERFs been regulated and how ERFs of different subgroup regulate the transcription of stress responsive genes, such as high-affinity K+ transporter gene PalHKT1;2, reactive oxygen species related genes LcLTP, LcPrx, and LcRP, flavonoids synthesis related genes FtF3H and LhMYBSPLATTER, etc. Though increasing researches demonstrate that ERFs are involved in various abiotic stresses, very few interact proteins and target genes of them have been comprehensively annotated. Hence, future research prospects are described on the mechanisms of how stress signals been transited to ERFs and how ERFs regulate the transcriptional expression of stress responsive genes.

show abstract

Section: Transcriptional Regulation Of Erfs In Response To Abiotic St...mentioning

confidence: 99%

Section: Transcriptional Regulation Of Erfs In Response To Abiotic St...mentioning

confidence: 99%

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

Zhang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…RNA transcript profiling can rapidly and effectively provide information for genome-wide transcript characterization, differential gene expression analysis, variant detection, and gene-specific expression. This technology is used to analyze the transcriptome in response to different biotic or abiotic stresses, including low-nitrogen stress [ 48 ], high-light stress [ 49 ], drought response to different soybean cultivars [ 50 ], salt stress [ 51 , 52 ], Cd stress [ 53 ], and sweet orange response to Citrus tristeza virus [ 54 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Tryptophan-Induced Resistance against Potato Common Scab

Zhao

Liu

Cao

et al. 2022

IJMS

View full text Add to dashboard Cite

Potato common scab (CS) is a worldwide soil-borne disease that severely reduces tuber quality and market value. We observed that foliar application of tryptophan (Trp) could induce resistance against CS. However, the mechanism of Trp as an inducer to trigger host immune responses is still unclear. To facilitate dissecting the molecular mechanisms, the transcriptome of foliar application of Trp and water (control, C) was compared under Streptomyces scabies (S) inoculation and uninoculation. Results showed that 4867 differentially expressed genes (DEGs) were identified under S. scabies uninoculation (C-vs-Trp) and 2069 DEGs were identified under S. scabies inoculation (S-vs-S+Trp). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that Trp induced resistance related to the metabolic process, response to stimulus, and biological regulation. As phytohormone metabolic pathways related to inducing resistance, the expression patterns of candidate genes involved in salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) pathways were analyzed using qRT-PCR. Their expression patterns showed that the systemic acquired resistance (SAR) and induced systemic resistance (ISR) pathways could be co-induced by Trp under S. scabies uninoculation. However, the SAR pathway was induced by Trp under S. scabies inoculation. This study will provide insights into Trp-induced resistance mechanisms of potato for controlling CS, and extend the application methods of Trp as a plant resistance inducer in a way that is cheap, safe, and environmentally friendly.

show abstract

“…NAC, a well-known flower-development-related TF ( Wang J et al., 2022 ), may also be involved in regulating NtCXE38 expression. miR156- NtCXE45 -ERF was another regulation module, and previous studies indicate that both miR156 and ERF are involved in drought tolerance ( González-Villagra et al, 2017 ; Yu et al., 2022 ). Our interaction network can further contribute to the functional research of NtCXE s. Notably, 36 NtCXE s can be targeted by miR169, which is involved in plant disease and abiotic stress regulation ( Luan et al., 2015 ; Hanemian et al., 2016 ), and NtCXE22 can be targeted by miR482, a miRNA superfamily that is critical for both disease resistance and plant development ( Zhang et al., 2022 ).…”

Section: Discussionmentioning

confidence: 88%

Comprehensive analysis of the carboxylesterase gene reveals that NtCXE22 regulates axillary bud growth through strigolactone metabolism in tobacco

Wang

Xie²,

Xu³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Carboxylesterases (CXE) are a class of hydrolytic enzymes with α/β-folding domains that play a vital role in plant growth, development, stress response, and activation of herbicide-active substances. In this study, 49 Nicotiana tabacum L. CXE genes (NtCXEs) were identified using a sequence homology search. The basic characteristics, phylogenetic evolution, gene structure, subcellular location, promoter cis-elements, and gene expression patterns of the CXE family were systematically analyzed. RNA-seq data and quantitative real-time PCR showed that the expression level of CXEs was associated with various stressors and hormones; gene expression levels were significantly different among the eight tissues examined and at different developmental periods. As a new class of hormones, strigolactones (SLs) are released from the roots of plants and can control the germination of axillary buds.NtCXE7, NtCXE9, NtCXE22, and NtCXE24 were homologous to Arabidopsis SLs hydrolase AtCXE15, and changes in their expression levels were induced by topping and by GR24 (a synthetic analogue of strigolactone). Further examination revealed that NtCXE22-mutant (ntcxe22) plants generated by CRISPR-Cas9 technology had shorter bud outgrowth with lower SLs content. Validation of NtCXE22 was also performed in NtCCD8-OE plants (with fewer axillary buds) and in ntccd8 mutant plants (with more axillary buds). The results suggest that NtCXE22 may act as an efficient SLs hydrolase and affects axillary bud development, thereby providing a feasible method for manipulating endogenous SLs in crops and ornamental plants.

show abstract

Transcriptomic Identification of Wheat AP2/ERF Transcription Factors and Functional Characterization of TaERF-6-3A in Response to Drought and Salinity Stresses

Cited by 31 publications

References 43 publications

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

Transcriptome Analysis of Tryptophan-Induced Resistance against Potato Common Scab

Comprehensive analysis of the carboxylesterase gene reveals that NtCXE22 regulates axillary bud growth through strigolactone metabolism in tobacco

Contact Info

Product

Resources

About