Transcriptomic investigation of the biochemical function of 7-dehydrocholesterol reductase 1 from the traditional Chinese medicinal plant Anemarrhena asphodeloides Bunge

Wang, Yunpeng; Xiong, Wenbo; Chen, Yidu; Zhu, Min; Liang, Jincai; Liu, Yu; Huang, Jia; Huang, Liufang; Liu, Zhongqiu; Ji, Aijia; Duan, Lixin

doi:10.1016/j.phytochem.2021.112954

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…In the present study, one CAS1 gene, one CYP 90 A1 gene, and one DET gene were expressed at higher levels in IM02 and IM03 than in IM01, which might lead to the production of peiminine, peimine, hupehenine, korseveramine, korseveridine, hericenones N-oxide, puqiedinone, delafrine, tortifoline, pingbeinone, puqienine B, puqienine E, pingbeimine A, jervine, and ussuriedine in the bulb development of F. hupehensis . In addition, 7-dehydrocholesterol reductase (7-DHCR) is a key enzyme that catalyzes the production of another cholesterol precursor ( Wang et al., 2021b ). In our study, one SQE gene and one 7-DHCR gene were downregulated in IM02 and IM03 compared with IM01.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and metabolomic analyses provide new insights into the appropriate harvest period in regenerated bulbs of Fritillaria hupehensis

Duan

Wu²,

Wang³

et al. 2023

Front. Plant Sci.

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IntroductionThe bulb of Fritillaria hupehensis, a traditional cough and expectorant medicine, is usually harvested from June to September according to traditional cultivation experience, without practical scientific guidance. Although steroidal alkaloid metabolites have been identified in F. hupehensis, the dynamic changes in their levels during bulb development and their molecular regulatory mechanisms are poorly understood.MethodsIn this study, integrative analyses of the bulbus phenotype, bioactive chemical investigations, and metabolome and transcriptome profiles were performed to systematically explore the variations in steroidal alkaloid metabolite levels and identify the genes modulating their accumulation and the corresponding regulatory mechanisms.ResultsThe results showed that weight, size, and total alkaloid content of the regenerated bulbs reached a maximum at IM03 (post-withering stage, early July), whereas peiminine content reached a maximum at IM02 (withering stage, early June). There were no significant differences between IM02 and IM03, indicating that regenerated bulbs could be harvested appropriately in early June or July. Peiminine, peimine, tortifoline, hupehenine, korseveramine, delafrine, hericenone N-oxide, korseveridine, puqiedinone, pingbeinone, puqienine B, puqienine E, pingbeimine A, jervine, and ussuriedine levels were upregulated in IM02 and IM03, compared with IM01 (vigorous growth stage, early April). The Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the accumulation of steroidal alkaloid metabolites mainly occurred prior to IM02. HMGR1, DXR, CAS1, CYP 90A1, and DET2 may play a positive role in peiminine, peimine, hupehenine, korseveramine, korseveridine, hericenone N-oxide, puqiedinone, delafrine, tortifoline, pingbeinone, puqienine B, puqienine E, pingbeimine A, jervine, and ussuriedine biosynthesis, whereas the downregulation of FPS1, SQE and 17-DHCR may lead to a reduction in peimisine levels. Weighted gene correlation network analysis showed that CYP 74A2-1, CYP 74A2-2, CYP 71A26-1, CYP 71A26-2, and CYP74A were negatively correlated with peiminine and pingbeimine A, whereas CYP R and CYP707A1 were positively correlated. . CYP 74A2-1 and CYP 74A2-2 may play a negative role in peimine and korseveridine biosynthesis, whereas CYP R plays a positive role. In addition, the highly expressed C2H2, HSF, AP2/ERF, HB, GRAS, C3H, NAC, MYB-related transcription factors (TFs), GARP-G2-like TFs, and WRKY may play positive roles in the accumulation of peiminine, peimine, korseveridine, and pingbeimine A.DiscussionThese results provide new insights into scientific harvesting of F. hupehensis.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and metabolomic analyses provide new insights into the appropriate harvest period in regenerated bulbs of Fritillaria hupehensis

Duan

Wu²,

Wang³

et al. 2023

Front. Plant Sci.

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“…Total saponins of anemarrhena (TSA) are a class of steroidal saponins (timosaponins) that originated from Anemarrhena asphodeloides Bge (Liliaceae) that applied for treatment of RA in China for thousands of years. Steroidal saponins in A. asphodeloides account for 6% of its total active components and include two types of furostanol glycosides and spirostanol glycosides (Lin et al, 2020; Y. Wang et al, 2021). Numbers study had shown that TSA could effectively alleviate synovial inflammation in RA (Kim et al, 2009; Lim et al, 2015; Wang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Sarsasapogenin, a principal active component absorbed into blood of total saponins of Anemarrhena, attenuates proliferation and invasion in rheumatoid arthritis fibroblast‐like synoviocytes through downregulating PKM2 inhibited pathological glycolysis

Dai

Liu

Wen

et al. 2023

Phytotherapy Research

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Increased glycolytic in fibroblast-like synoviocytes (FLS) of rheumatoid arthritis (RA) not only contributes to early-stage disease pathogenesis but leads to sustained proliferation of FLS. Given the importance of PKM2 in glycolysis and apoptosis, PKM2 is considered a potential therapeutic and drug discovery target in RA. Total saponins of anemarrhena (TSA), a class of steroid saponins, originated from Anemarrhena asphodeloides Bge. In this study, we verified that 200 mg/kg TSA could significantly alleviate inflammation and the pathological characteristics of RA and inhibit synovial hyperplasia in AA rats. We confirmed that sarsasapogenin (SA) was the principal active ingredient absorbed into the blood of TSA by the UPLC/Q Exactive MS test. Then we used TNF-α-induced MH7A to get the conclusion that 20 μM SA could effectively inhibit the glycolysis by inhibiting the activity of PKM2 tetramer and glucose uptake. Moreover, 20 μM SA could suppress proliferation, migration, invasion, and cytokine release of FLS, interfere with the growth cycle of FLS, and induce FLS apoptosis by depressing the phosphorylation of PKM2. At last, In-1, a potent inhibitor of the PKM2 was used to reverse verify the above results. Taken together, the key mechanisms of SA on RA treatment through downregulating the activity of PKM2 tetramer and phosphorylation of PKM2 inhibited pathological glycolysis and induced apoptosis to exert inhibition on the proliferation and invasion of RA FLS.

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Anemarrhena asphodeloides Bunge total saponins lower lipid via modulating MAOA activity to enhance defense mechanisms in mice and C. elegans

Shi,

Jing,

Feng

et al. 2025

Journal of Ethnopharmacology

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Transcriptomic investigation of the biochemical function of 7-dehydrocholesterol reductase 1 from the traditional Chinese medicinal plant Anemarrhena asphodeloides Bunge

Cited by 3 publications

References 37 publications

Transcriptomic and metabolomic analyses provide new insights into the appropriate harvest period in regenerated bulbs of Fritillaria hupehensis

Transcriptomic and metabolomic analyses provide new insights into the appropriate harvest period in regenerated bulbs of Fritillaria hupehensis

Sarsasapogenin, a principal active component absorbed into blood of total saponins of Anemarrhena, attenuates proliferation and invasion in rheumatoid arthritis fibroblast‐like synoviocytes through downregulating PKM2 inhibited pathological glycolysis

Anemarrhena asphodeloides Bunge total saponins lower lipid via modulating MAOA activity to enhance defense mechanisms in mice and C. elegans

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