Transcriptomic profile of VEGF-regulated genes in human cervical epithelia

Johnson, MacKinsey Diane; Mowa, Chishimba Nathan

doi:10.1007/s00441-020-03354-y

Cited by 4 publications

(4 citation statements)

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“…The expression heatmap showed dozens of genes associated with mitochondrial dysfunction were downregulated in M1 macrophages (Figure 2B). Many of these genes are essential for the maintenance of mitochondrial biogenesis and energy production, including MT‐CO3, [ 37 ] MTATP6P1, [ 38 ] MT‐ATP6, [ 39 ] MT‐CYB, [ 40 ] MT‐ND4, [ 41 ] RNR2, [ 42 ] and MT‐DN1. [ 43 ] Moreover, MT‐ND3 [ 44 ] and MT‐CO3 [ 37,45 ] can be regulated by hypoxia, and their dysfunction is related to oxidative stress, oxygen supply, and lipid metabolism (Figure 2C, Figure S4, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Reprogramming Mitochondrial Metabolism in Synovial Macrophages of Early Osteoarthritis by a Camouflaged Meta‐Defensome

et al. 2022

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Osteoarthritis (OA) is a low‐grade inflammatory and progressive joint disease, and its progression is closely associated with an imbalance in M1/M2 synovial macrophages. Repolarizing pro‐inflammatory M1 macrophages into the anti‐inflammatory M2 phenotype is emerging as a strategy to alleviate OA progression but is compromised by unsatisfactory efficiency. In this study, the reprogramming of mitochondrial dysfunction is pioneered with a camouflaged meta‐Defensome, which can transform M1 synovial macrophages into the M2 phenotype with a high efficiency of 82.3%. The meta‐Defensome recognizes activated macrophages via receptor–ligand interactions and accumulates in the mitochondria through electrostatic attractions. These meta‐Defensomes are macrophage‐membrane‐coated polymeric nanoparticles decorated with dual ligands and co‐loaded with S‐methylisothiourea and MnO2. Meta‐Defensomes are demonstrated to successfully reprogram the mitochondrial metabolism of M1 macrophages by scavenging mitochondrial reactive oxygen species and inhibiting mitochondrial NO synthase, thereby increasing mitochondrial transcription factor A expression and restoring aerobic respiration. Furthermore, meta‐Defensomes are intravenously injected into collagenase‐induced osteoarthritis mice and effectively suppress synovial inflammation and progression of early OA, as evident from the Osteoarthritis Research Society International score. Therefore, reprogramming the mitochondrial metabolism can serve as a novel and practical approach to repolarize M1 synovial macrophages. The camouflaged meta‐Defensomes are a promising therapeutic agent for impeding OA progression in tclinic.

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Section: Resultsmentioning

confidence: 99%

Reprogramming Mitochondrial Metabolism in Synovial Macrophages of Early Osteoarthritis by a Camouflaged Meta‐Defensome

et al. 2022

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“…We hypothesize that under low oxygen culture at VEGF ligand concentrations resembling those found in the antral follicle, granulosa cells will upregulate VEGF ligand and downregulate VEGF receptor mRNA expression (Hernández-Morales et al, 2021). Estradiol increases the expression of the VEGF gene in normal human endometrium, also prostaglandin is another factor known to up-regulate VEGF expression (Johnson and Mowa, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…vasculogenesis(Porwal et al, 2021) and its expression increases as gestation proceeds. The hypoxiainducible factor 1 (HIF1A) controls the expression and transcription of VEGF, therefore, the clock network regulates angiogenesis and embryonic development(Johnson and Mowa, 2021). Under low oxygen conditions, HIF1A activates multiple transcription factors, including VEGF which promotes angiogenesis and vascularization in the placenta(Fong, 2008, Agaoglu et al, 2015.…”

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confidence: 99%

Predictive biochemical and oxidative markers for dairy cows with and without retained fetal placenta

Elmetwally¹,

Shalapy

Montaser

2022

Preprint

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The changes in the expression profile of the angiogenicgenes and serum biomarkers were investigated in the cows with the retained placenta after normal parturition. Retained fetal membranes (RFM) are considered one of the main reproductive disorders in dairy cattle. Highlight the effects of Excede® (Ceftiofur Crystalline Free Acid: Zoetis, USA) in combination with Oxytocin (Oxytocin®, each ml contains 10 IU oxytocin, ADWIA, Egypt), Estrumate® (250µg cloprostenol: synthetic analogue of PGF2ά) and Flunixin Meglumine® (nonsteroidal anti-inflammatory drug) on the reproductive performance in dairy cows. A total of 16 cows were allocated into two groups: group 1 (n = 8) the cows that have a normal loosed placenta, and group 2 (n = 8). The cows that have retained fetal membranes for more than 24 h after parturition. Blood with EDTA was collected from the tail vein for studying the expression of angiogenic proteins including vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor1 (VEGFR1), and RANTES genes. Serum was collected at a one-week interval before/after parturition for the study of biochemical changes. The VEGF expression decreased ten folds in cows with retained than normally dropped placentae (p ˂ 0.0001). The vascular endothelial growth factor receptor2 (VEGFR2) showed a threefold increase in cows with normal loosed than retained placentae (p˂0.01). The RANTES was increased in cows with retained rather than normal placentae by about 7 folds (p˂0.01). Phosphorus and total proteins were significantly lower (p˂0.05) in RFM cows one week after parturition. The serum albumin, TG, HDL, and cholesterol were significantly lower (p˂0.05) in RFM before, at, and after parturition. CPK was significantly higher in RFM before, at, and after parturition (p˂0.05). Increased values of MDA and decreased values of CAT, SOD, and R-GSH were detected in the blood of cows suffering from RFM (p˂0.05). Reproductive performance parameters for all studied cows were days to first estrous, number of service per conception and pregnancy rate. All reproductive parameters demonstrated significant (P < 0.05) changes between treated animals. The translation of VEGF and its receptor and RANTESmRNA at the time of parturition in dairy cows could be proxy biomarkers for the prediction of retained fetal membranes (RFM). In the same line, the activity of total proteins, serum albumin, TG, HDL, cholesterol, and CPK would be useful in the prediction of the RFM in dairy cows. Altogether, the obtained results indicated that retained fetal membranes in cows could be treated successfully without any interference by systemic injection of cephalosporin with caring of temperature. The reproductive performance criteria for all cows studied were the time of first estrus, the number of services per conception, and pregnancy rates. All parameters of reproductive performance showed significant changes (P < 0.05) among the treated animals. The results also indicated that cephalosporin can be used by systemic injection to treat placental retention with caring of the animal's temperature.

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“…Existen pocos estudios que evalúen los cambios de la vascularización cervical durante el embarautilizando ecografía tridimensional, angiografía Doppler de potencia y el método VOCAL (15,20) . Los factores angiogénicos parecen tener un papel fundamental tanto en la maduración cervical como en el proceso del parto, por lo que es posible que los índices vasculares pudiesen ser utilizados para identificar los cambios previos a las modificaciones cervicales y al parto, ya sea pretérmino o a término (21) . Algunos reportes han propuesto que los parámetros vasculares cervicales podrían ser útiles para establecer con precisión qué embarazadas tienen riesgo de iniciar el trabajo de parto.…”

Section: Discussionunclassified

Índices de vascularización cervical en la predicción de parto pretérmino inminente

Mejia-Montilla¹,

Reyna-Villasmil²,

Torres-Cepeda³

et al. 2022

Rev peru ginecol obstet.

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Objetivo. Establecer la utilidad de los índices de vascularización cervical en la predicción de parto pretérmino inminente. Diseño. Estudio de casos-controles. Institución. Hospital Central “Dr. Urquinaona”, Maracaibo, Venezuela. Participantes. Pacientes con parto pretérmino en los siguientes 7 días (grupo A) y embarazadas con parto pretérmino más allá de los 7 días (grupo B). Métodos. Las embarazadas fueron evaluadas utilizando ecografía transvaginal y seguidas hasta el parto. Principales medidas de resultado. Características generales, índices de vascularización cervical (índice de vascularización, índice de flujo e índice de vascularización / flujo), parto pretérmino inminente y eficacia pronóstica. Resultados. Fueron incluidas 350 pacientes, 75 mujeres presentaron parto pretérmino inminente (grupo A) y 251 pacientes fueron consideradas como controles (grupo B). Las pacientes del grupo A tuvieron valores significativamente más bajos del índice de vascularización e índice de flujo comparado con el grupo B (p = 0,0122 y p < 0,0001, respectivamente). Las pacientes del grupo B presentaron valores significativamente más altos de índice de vascularización / flujo comparadas con las pacientes del grupo A (p = 0,0103). Los tres índices y la combinación de estos no mostraron capacidad de discriminación de parto pretérmino inminente. Conclusiones. Las pacientes con parto pretérmino inminente presentan diferencias significativas en los índices de vascularización comparado con las pacientes que presentan parto pretérmino más allá de los 7 días de la evaluación ecográfica. Sin embargo, no son útiles en la predicción del parto pretérmino inminente.

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Transcriptomic profile of VEGF-regulated genes in human cervical epithelia

Cited by 4 publications

References 73 publications

Reprogramming Mitochondrial Metabolism in Synovial Macrophages of Early Osteoarthritis by a Camouflaged Meta‐Defensome

Reprogramming Mitochondrial Metabolism in Synovial Macrophages of Early Osteoarthritis by a Camouflaged Meta‐Defensome

Predictive biochemical and oxidative markers for dairy cows with and without retained fetal placenta

Índices de vascularización cervical en la predicción de parto pretérmino inminente

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