Transcriptomic profiles of spotted knifejaw (Oplegnathus punctatus) spleen in response to Megalocytivirus infection

Xu, Wenteng; Li, Ming; Wang, Shan; Song, Yu; Xu, Hao; Lin, Kueiyu Joshua; Wang, Jie; Chen, Songlin

doi:10.1016/j.aquaculture.2022.738212

Cited by 2 publications

(2 citation statements)

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“…Comprehensive gene expression analysis in the spleen associated with RSIV or RSIV‐like Megalocytivirus infection has been conducted. After the virus infection, a series of inflammatory signalling molecules were upregulated in the spleen of spotted knifejaw and Mandarin fish (Xu et al, 2022; Zhang et al, 2023). The findings are consistent with the gene expression patterns in groups 1 and 2 (see Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…gene expression, red sea bream iridovirus, rock bream (Oplegnathus fasciatus), transcriptome Further, the host gene expression profiles against Megalocytivirus infection have been investigated. For example, transcriptomic analysis in the spleen of rock bream (Oplegnathus fasciatus) (Kim et al, 2020), spotted knifejaw (Oplegnathus punctatus) (Xu et al, 2022) and Mandarin fish (Siniperca chuatsi) (Zhang et al, 2023) following RSIV infection revealed changes in the expression of hundreds of genes and provided information regarding the interaction between the host and virus. However, these analyses sampled only a time point after the challenge, and the expression kinetics of each gene have not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Red sea bream iridovirus infection downregulates inflammation‐related genes in the spleen of rock bream (Oplegnathus fasciatus)

Eurlaphan,

Nozaki,

Sano

et al. 2023

Journal of Fish Diseases

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This study investigated the kinetics of red sea bream iridovirus and host gene expression during infection in rock bream (Oplegnathus fasciatus), a species highly sensitive to the virus. After intraperitoneal injection of the viral solution at 104 TCID50/fish, the viral genome copy number in the spleen was 104.7 ± 0.2 and 105.9 ± 0.4 copies/μg DNA at 3 and 5 days post‐injection (dpi), respectively. Using transcriptomic analyses via MiSeq, viral gene transcripts were detected at 3 and 5 dpi. Six genes including RING‐finger domain‐containing protein and laminin‐type epidermal growth factor‐like domain genes were significantly expressed at 5 dpi. Further, 334 host genes were differentially expressed compared with those before infection. Genes were clustered into four groups based on their expression profiles. Interferon‐stimulated genes were more prevalent in groups showing upregulation at 5 dpi and 3 and 5 dpi. In contrast, the group showing downregulation at 3 dpi included inflammation‐related genes, such as granzyme and eosinophil peroxidase genes. Downregulation of certain inflammation‐related genes may contribute to the susceptibility of this fish to the virus.

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Section: Resultsmentioning

confidence: 99%