Transcriptomic Profiles of Vibrio parahaemolyticus During Biofilm Formation

Zhang, Yiquan; Zhang, Tingting; Qiu, Yue; Zhang, Miaomiao; Lu, Xiuhui; Yang, Wenhui; Hu, Lingfei; Zhou, Dongsheng; Gao, Bo; Lu, Renfei

doi:10.1007/s00284-023-03425-7

Cited by 9 publications

(3 citation statements)

References 110 publications

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“…Furthermore, we investigated the effects of IPBC on the expression of biofilm- or virulence-related genes to gain possible mechanistic insight. In V. parahaemolyticus , the expression of cpsA involved in capsular polysaccharide production for biofilm formation 52 , the mannose-sensitive hemagglutinin ( mshA ), fliA and opaR encoding type IV pili, flagella sigma factor and QS, respectively 7 , 53 were repressed (Fig. 7 A), which partially explains the inhibitory effect of IPBC on biofilm formation and virulence factors.…”

Section: Discussionmentioning

confidence: 97%

“…Furthermore, V. parahaemolyticus possesses a number of virulence factors, including polar and lateral flagella, and adhesion factors that increase its ability to invade hosts, attach to surfaces, and produce biofilms 6 . V. parahaemolyticus in biofilm state is capable of enhanced virulence 7 and its transcriptional protein ToxR directly regulates both biofilm formation and thermostable direct hemolysin 4 , 8 .…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial and antibiofilm activities of iodinated hydrocarbons against Vibrio parahaemolyticus and Staphylococcus aureus

Faleye,

Lee

et al. 2024

Sci Rep

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Food-related illnesses have become a growing public concern due to their considerable socioeconomic and medical impacts. Vibrio parahaemolyticus and Staphylococcus aureus have been implicated as causative organisms of food-related infections and poisoning, and both can form biofilms which confer antibiotic resistance. Hence, the need for continuous search for compounds with antibiofilm and antivirulence properties. In this study, 22 iodinated hydrocarbons were screened for their antibiofilm activity, and of these, iodopropynyl butylcarbamate (IPBC) was found to effectively control biofilm formation of both pathogens with a MIC of 50 µg/mL which was bactericidal to V. parahaemolyticus and S. aureus. Microscopic studies confirmed IPBC inhibits biofilm formation of both bacteria and also disrupted their mixed biofilm formation. Furthermore, IPBC suppressed virulence activities such as motility and hemolytic activity of V. parahaemolyticus and the cell surface hydrophobicity of S. aureus. It exhibited a preservative potential against both pathogens in a shrimp model. IPBC disrupted the cell membrane of S. aureus and V. parahaemolyticus and differentially affected gene expressions related to biofilm formation and virulence. Additionally, it displayed broad-spectrum antibiofilm activities against other clinically relevant pathogens. These findings indicate IPBC offers a potential means of controlling infections mediated by Vibrio and Staphylococcus biofilms.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Antibacterial and antibiofilm activities of iodinated hydrocarbons against Vibrio parahaemolyticus and Staphylococcus aureus

Faleye,

Lee

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…CV staining assay was performed similarly as previously described [ [43] , [44] , [45] , [46] , [47] ]. Briefly, the bacterial seed was diluted 50-fold into 2 ml HI broth or HI broth supplemented with 55 mM Mg 2+ in a 24-well cell culture plate, and then incubated at 30 °C with shaking at 150 rpm for 12 h. Planktonic cells were collected for detection of OD 600 values.…”

Section: Methodsmentioning

confidence: 99%