Transcriptomic profiling analysis of human endometrial stromal cells treated with autologous platelet‐rich plasma

Abstract

Purpose To clarify the mechanisms of intrauterine platelet‐rich plasma (PRP) infusion that support embryo implantation in in vitro fertilization treatment. Methods Blood and endometrial samples were collected from four infertile women. Human endometrial stromal cells (HESCs) were cultured and passaged equally into four cell culture dishes in each patient. Two were treated with PRP twice, and the other two were treated with vehicle. Subsequently, two cultures with and without PRP were decidualized with 8‐bromoadenosine 3′,5′‐cyclic AMP and progesterone for 5 days. Results The gene expression in undifferentiated or decidualized HESCs with and without PRP was compared. In the microarray analysis, 381 and 63 differentially expressed genes were detected in undifferentiated and decidualized HESCs, respectively. In the undifferentiated HESCs, PRP was found to promote the gene expression associated with cell growth, tissue regeneration, proinflammatory response, and antibiotic effects. In decidualized HESCs, PRP was found to attenuate the gene expression involved in cell proliferation and inflammation by inhibiting the expression of phosphoinositide 3‐kinase signaling. Conclusions Platelet‐rich plasma regulates the reprogramming of cell proliferation and inflammation depending on menstrual cycle phases in an appropriate manner, suggesting that PRP has the potential to increase endometrial thickness in the proliferative phase and improve immune tolerance in the secretory phase.

Glucose and lipid metabolisms in human endometrial stromal cells during decidualization

“…This process is critical for embryo implantation and the establishment of pregnancy because a defective decidualization response is associated with reproductive disorders such as early pregnancy loss and recurrent miscarriage [3,4]. Decidualization is regulated by many factors, including intracellular signaling pathways, their related molecules, and transcription factors [5][6][7][8][9][10][11][12]. These factors cause dramatic changes in gene expressions.…”

Current perspectives on endometrial receptivity: A comprehensive overview of etiology and treatment

“…Chang et al reported that PRP administration improved the immunological environment in the endometrium with intrauterine adhesions 136 . In an in vitro study, Kuroda et al concluded that PRP therapy might contribute to endometrial thickening during the proliferative phase 137 . However, there is still conflicting evidence regarding the clinical pregnancy rate in patients with RIF receiving PRP therapy 138 .…”

“…136 In an in vitro study, Kuroda et al concluded that PRP therapy might contribute to endometrial thickening during the proliferative phase. 137 However, there is still conflicting evidence regarding the clinical pregnancy rate in patients with RIF receiving PRP therapy. 138 Hence, further largescale prospective and high-quality clinical studies should be conducted to determine the efficacy and indication of PRP therapy in infertile women with thin endometria.…”

Management strategies following implantation failure of euploid embryos

“… 44 Moreover, in vivo and in vitro studies showed that intrauterine platelet‐rich plasma (PRP) infusion also had endometrial proliferative effects. 45 , 46 Meanwhile, another meta‐analysis showed that PRP infusion significantly increased endometrial thickness and improved clinical pregnancy rates in women with thin endometrium or RIF (thin endometrium, RR: 1.79, 95% CI: 1.37–2.32; RIF, RR: 1.73, 95% CI: 1.24–2.41). 47 Overall, evidence suggests that regenerative medicine has potential therapeutic efficacy for RIF with an abnormal intrauterine environment.…”