Transcriptomic Profiling Highlights the ABA Response Role of BnSIP1-1 in Brassica napus

Abstract

BnSIP1-1 is the first identified SIP1 (6b Interacting Protein1) subfamily gene of the trihelix transcription factor family from Brassica napus (B. napus). We previously used a reverse genetic method to reveal its abiotic stress response function in endowing plants resistance to drought and salinity, as well as ABA (Abscisic acid). However, the molecular mechanisms of BnSIP1-1 are unclear. In this study, the global transcriptome files of BnSIP1-1-overexpressing transgenic and wildtype B. napus seedlings under ABA treatment were constructed using RNA-seq. A total of 1823 and 5512 DEGs (Differentially Expressed Genes) were identified in OE vs. WT and OE_ABA vs. WT_ABA comparison groups, which included 751 and 2567 up-regulated DEGs, and 1072 and 2945 down-regulated DEGs, separately. The impact of overexpressed BnSIP1-1 on plants was amplified by ABA, indicating BnSIP1-1 was an ABA-conditioned responsive gene. More interestingly, we found the reasons for BnSIP1-1 increasing plants’ insensitivity to ABA were not by regulating ABA synthesis and catabolism, but by manipulating ABA transportation, ABA signal perception and transduction, inositol phosphate metabolism, as well as endomembrane trafficking, indirectly suggesting this gene may play roles upstream of the core ABA response pathway. Our results provided new insights into improving the knowledge about the function of BnSIP1-1 and the ABA signaling mechanism in B. napus.

Construction and Identification of Cold Tolerance in Different Broccoli Cultivars at the Seedling Stage

“…At the same time, low temperature also affects the synthesis of chlorophyll, leading to a decrease in the photosynthetic rate and changes in the content of soluble sugars, H 2 O 2 , and other substances in plants [6][7][8][9]. Most of the existing research on low temperature in broccoli has focused on vernalization and the impact of exogenous factors on broccoli plants, such as the effects of exogenous Ca 2+ and ABA on broccoli under low-temperature stress [10][11][12]. There is very little research on the comprehensive identification of low-temperature tolerance, and plant low-temperature tolerance is a comprehensive trait that requires the use of a combination of multiple methods to avoid the one-sided evaluation of plant low-temperature tolerance using only a single indicator of change.…”