Transcriptomic profiling of cotton Gossypium hirsutum challenged with low-temperature gradients stress

Li, Zhibo; XiaoYan, Zeng; Xu, Jianwei; Zhao, Ruihai; Yinong, Wei

doi:10.1038/s41597-019-0210-7

Cited by 16 publications

(14 citation statements)

References 19 publications

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“…The results showed that GhRPS6 (Gh_D13G0519.1) of G. hirsutum was in the same branch as GOBAR DD31969 of G. barbadense, and the protein sequence was closest to that of A. thaliana AT4G31700 ( Figure 1a). Upland cotton (G. hirsutum) is the most widely cultivated species globally [31]. To better understand the evolutionary relationship between different members of the GhRPS6 gene family, we constructed separate rootless phylogenetic trees using GhRPS6 DNA sequences and performed comparative analysis of intron-exon structures (Figure 1b,d).…”

Section: Ghrps6 Gene Cloning and Bioinformatics Analysismentioning

confidence: 99%

Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance

Zhu

Zhang

Zhou

et al. 2021

IJMS

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Verticillium wilt is threatening the world’s cotton production. The pathogenic fungus Verticillium dahliae can survive in the soil in the form of microsclerotia for a long time, colonize through the root of cotton, and invade into vascular bundles, causing yellowing and wilting of cotton leaves, and in serious cases, leading to plant death. Breeding resistant varieties is the most economical and effective method to control Verticillium wilt. In previous studies, proteomic analysis was carried out on different cotton varieties inoculated with V. dahliae strain Vd080. It was found that GhRPS6 was phosphorylated after inoculation, and the phosphorylation level in resistant cultivars was 1.5 times than that in susceptible cultivars. In this study, knockdown of GhRPS6 expression results in the reduction of SA and JA content, and suppresses a series of defensive response, enhancing cotton plants susceptibility to V. dahliae. Overexpression in Arabidopsis thaliana transgenic plants was found to be more resistant to V. dahliae. Further, serines at 237 and 240 were mutated to phenylalanine, respectively and jointly. The transgenic Arabidopsis plants demonstrated that seri-237 compromised the plant resistance to V. dahliae. Subcellular localization in Nicotiana benthamiana showed that GhRPS6 was localized in the nucleus. Additionally, the pathogen inoculation and phosphorylation site mutation did not change its localization. These results indicate that GhRPS6 is a potential molecular target for improving resistance to Verticillium wilt in cotton. This lays a foundation for breeding disease-resistant varieties.

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Section: Ghrps6 Gene Cloning and Bioinformatics Analysismentioning

confidence: 99%

Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance

Zhu

Zhang

Zhou

et al. 2021

IJMS

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“…Many characters have been targeted to find the key responsible genes and pathways, especially fiber initiation, and elongation being highly focused up on [61][62][63][64][65][66][67][68]. Other characters like Green and Brown colored cotton [69-71], Cadmium tolerance [72], Cold stress [73], Drought stress [74], Nematode resistance [75], Semigamy in Pima cotton [76], Whitefly mediated cotton leaf curl infection transcriptome [77], the transcriptome of Mepiquat chloride-induced compact types using in cotton [78] have also been done in cotton. Many studies have been carried out to identify genes that are differentially expressed, however paucity of the causes of the differential expression, hints us towards epigenetic regulations and transcription factors as the probable cause.…”

Section: Transcriptome Studies In Cottonmentioning

confidence: 99%

Prospects for Molecular Breeding in Cotton,Gossypiumspp

Katageri¹,

Gowda²,

B.N³

et al. 2021

Plant Breeding - Current and Future Views

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Conventional breeding interventions in cotton have been successful and these techniques have doubled the productivity of cotton, but it took around 40 years. One of the techniques of molecular biology i.e., genetic engineering has brought significant improvement in productivity within the year of introduction. With cotton genomics maturing, many reference genomes and related genomic resources have been developed. Newer wild species have been discovered and many countries are conserving genetic resources within and between species. This valuable germplasm can be exchanged among countries for increasing cotton productivity. As many as 249 Mapping and Association studies have been carried out and many QTLs have been discovered and it is high time for researchers to get into fine-mapping studies. Techniques of genomic selection hold valuable trust for deciphering quantitative traits like fiber quality and productivity since they take in to account all minor QTLs. There are just two studies involving genomic selection in cotton, underlining its huge prospects in cotton research. Genome editing and transformation techniques have been widely used in cotton with as many as 65 events being developed across various characters, and eight studies carried out using crisper technology. These promising technologies have huge prospects for cotton production sustainability.

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“…In rice, transcriptomic analysis has been used to study the cold response mechanisms of leaves, roots, and shoots at the seedling stage, as well as anthers at the reproductive stage [30][31][32]. Recently, scientists have studied the cold response mechanisms of true cotton leaves at the seedling stage and seed embryos at the germination stage by using RNA-seq technology [3,33]. However, reports on the transcriptome profiling of cotton seedling cotyledons under cold stress had not been disclosed.…”

Section: Introductionmentioning

confidence: 99%

“…They are not only the essential autotrophic organs before the first true leaf appeared but also the most vulnerable parts to cold stress. Previous studies on the response of cotton to cold stress mainly focused on the germination stage of seeds and the true leaf stage of seedlings [3,33]. However, the seedling cotyledons are more susceptible to cold stress than true leaves.…”

Section: Introductionmentioning

confidence: 99%

“…Low temperature is detrimental to plant growth and survival, as well as the yield and quality of thermophilic crops. As a commercial crop originating in low-latitude regions, the optimum growth temperature of cotton is 20–30 °C [ 3 ]. However, different varieties of the same plant species are generally with different tolerance to cold stress [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Profiling of Young Cotyledons Response to Chilling Stress in Two Contrasting Cotton (Gossypium hirsutum L.) Genotypes at the Seedling Stage

Cheng

Zhang

Wang

et al. 2020

IJMS

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Young cotyledons of cotton seedlings are most susceptible to chilling stress. To gain insight into the potential mechanism of cold tolerance of young cotton cotyledons, we conducted physiological and comparative transcriptome analysis of two varieties with contrasting phenotypes. The evaluation of chilling injury of young cotyledons among 74 cotton varieties revealed that H559 was the most tolerant and YM21 was the most sensitive. The physiological analysis found that the ROS scavenging ability was lower, and cell membrane damage was more severe in the cotyledons of YM21 than that of H559 under chilling stress. RNA-seq analysis identified a total of 44,998 expressed genes and 19,982 differentially expressed genes (DEGs) in young cotyledons of the two varieties under chilling stress. Weighted gene coexpression network analysis (WGCNA) of all DEGs revealed four significant modules with close correlation with specific samples. The GO-term enrichment analysis found that lots of genes in H559-specific modules were involved in plant resistance to abiotic stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that pathways such as plant hormone signal transduction, MAPK signaling, and plant–pathogen interaction were related to chilling stress response. A total of 574 transcription factors and 936 hub genes in these modules were identified. Twenty hub genes were selected for qRT-PCR verification, revealing the reliability and accuracy of transcriptome data. These findings will lay a foundation for future research on the molecular mechanism of cold tolerance in cotyledons of cotton.

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Transcriptomic profiling of cotton Gossypium hirsutum challenged with low-temperature gradients stress

Cited by 16 publications

References 19 publications

Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance

Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance

Prospects for Molecular Breeding in Cotton,Gossypiumspp

Transcriptomic Profiling of Young Cotyledons Response to Chilling Stress in Two Contrasting Cotton (Gossypium hirsutum L.) Genotypes at the Seedling Stage

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