Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril

Abstract

Fusarium pseudograminearum has been identified as a significant pathogen. It causes Fusarium crown rot (FCR), which occurs in several major wheat-producing areas in China. Chemical control is the primary measure with which to control this disease. In this study, transcriptome sequencing (RNA-Seq) was used to determine the different mechanisms of action of four frequently used fungicides including carbendazim, pyraclostrobin, tebuconazole, and phenamacril on F. pseudograminearum. In brief, 381, 1896, 842, and 814 differentially expressed genes (DEGs) were identified under the carbendazim, pyraclostrobin, tebuconazole, and phenamacril treatments, respectively. After the joint analysis, 67 common DEGs were obtained, and further functional analysis showed that the ABC transported pathway was significantly enriched. Moreover, FPSE_04130 (FER6) and FPSE_11895 (MDR1), two important ABC multidrug transporter genes whose expression levels simultaneously increased, were mined under the different treatments, which unambiguously demonstrated the common effects. In addition, Mfuzz clustering analysis and WGCNA analysis revealed that the core DEGs are involved in several critical pathways in each of the four treatment groups. Taken together, these genes may play a crucial function in the mechanisms of F. pseudograminearum‘s response to the fungicides stress.

Genomic insights into Aspergillus sydowii 29R-4-F02: unraveling adaptive mechanisms in subseafloor coal-bearing sediment environments

“…Among them, OG0000029 and OG0000322 were involved in ZEB2-regulated ABC transporter and glycopeptide α-N-acetylgalactosaminidase, respectively. ZEB2 regulated ABC transporters have been shown to play a crucial role in secreting virulence factors or compounds in pathogenic fungi ( Lee et al, 2011 ; Zhang et al, 2023 ). Glycopeptide α-N-acetylgalactosaminidase belongs to glycoside hydrolase family (GH101) and has been primarily demonstrated to hydrolyze core 1-type O-glycans from glycoproteins ( Fujita et al, 2005 ).…”

Involvement of AoMdr1 in the Regulation of the Fluconazole Resistance, Mycelial Fusion, Conidiation, and Trap Formation of Arthrobotrys oligospora

“…They contain both ABC_tran and two ABC_membrane structural domains in different fungi [ 2 ] as well as high similarities in protein sequences, indicating that they are extremely conserved in different fungi. Previous studies have shown that Mdr1 is not only involved in drug resistance but also in regulating the pathogenicity of fungi; it has an important role in growth, development, and adaptation to stressful environments [ 45 , 46 , 47 ]. In this study, an orthologous Mdr protein, AoMdr1, was identified in the NT fungus A. oligospora , and its function was investigated.…”

Comprehensive Genome and Transcriptome Analysis Identifies SLCO3A1 Associated with Aggressive Behavior in Pigs

“…Differentially expressed genes (DEGs) were identified using the R package DEseq2, employing the criteria of fold change ≥2 or ≤0.5 and a p -value < 0.05 [ 27 ]. Kmeans and Mfuzz methods were used to perform clustering analysis for the differential genes of the MAP and LAP [ 28 , 29 ]. To further explore the functional implications, gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) were conducted using the clusterProfiler and GSVA of the R package [ 30 ].…”