Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection

Boba, Aleksandra; Kostyń, Kamil; Kozak, Bartosz; Zalewski, Iwan; Szopa, Jan; Kulma, Anna

doi:10.1371/journal.pone.0246052

Cited by 13 publications

(14 citation statements)

References 45 publications

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“…The resistant plants exhibited a sharp, prolonged response to the infection as early as the third dpi. In line with previous work [9][10][11], we observed changes in expression of genes controlling membrane remodelling, secondary metabolite synthesis, synthesis of lipids and cytochrome-related proteins, transcription factors in response to infection. Furthermore, an activation of Germin-like genes provides a clear indication of flax response to the biotic stress associated with disease progression.…”

supporting

confidence: 91%

Transcriptional profiling of fusarium wilt in flax

2022

The Thirteenth International Multiconference

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supporting

confidence: 91%

Transcriptional profiling of fusarium wilt in flax

2022

The Thirteenth International Multiconference

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“…Pathogen-related proteins, components of the cell wall, transcription factors, secondary metabolites and antioxidants all play essential roles in the response of flax to the F. oxysporum f.sp. lini infection, as demonstrated by several transcriptomics experiments [19][20][21].…”

Section: Introductionmentioning

confidence: 75%

“…Recently, several high-throughput transcriptomics experiments provided a general insight into flax resistance to pathogens [19][20][21]. However, the identification of potential key candidate genes in the control of resistance is challenging due to a systemic effect inflicted by infection and, consequently, an unfeasibly large number of genes to be interrogated in focused follow-up studies.…”

Section: Discussionmentioning

confidence: 99%

Genomic Regions Associated with Fusarium Wilt Resistance in Flax

Kanapin

Bankin

Rozhmina

et al. 2021

IJMS

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Modern flax cultivars are susceptible to many diseases; arguably, the most economically damaging of these is the Fusarium wilt fungal disease. Over the past decades international flax breeding initiatives resulted in the development of resistant cultivars. However, much remains to be learned about the mechanisms of resistance to Fusarium infection in flax. As a first step to uncover the genetic factors associated with resistance to Fusarium wilt disease, we performed a genome-wide association study (GWAS) using 297 accessions from the collection of the Federal Research Centre of the Bast Fiber Crops, Torzhok, Russia. These genotypes were infected with a highly pathogenic Fusarium oxysporum f.sp. lini MI39 strain; the wilt symptoms were documented in the course of three successive years. Six different single-locus models implemented in GAPIT3 R package were applied to a selected subset of 72,526 SNPs. A total of 15 QTNs (Quantitative Trait Nucleotides) were detected during at least two years of observation, while eight QTNs were found during all three years of the experiment. Of these, ten QTNs occupied a region of 640 Kb at the start of chromosome 1, while the remaining QTNs mapped to chromosomes 8, 11 and 13. All stable QTNs demonstrate a statistically significant allelic effect across 3 years of the experiment. Importantly, several QTNs spanned regions that harbored genes involved in the pathogen recognition and plant immunity response, including the KIP1-like protein (Lus10025717) and NBS-LRR protein (Lus10025852). Our results provide novel insights into the genetic architecture of flax resistance to Fusarium wilt and pinpoint potential candidate genes for further in-depth studies.

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“…Earlier study reported that transgenic flax plants overexpressing the β-1,3-glucanase gene showed lower susceptibility to this pathogen ( Wróbel-Kwiatkowska et al, 2004 ). The transcriptomal response of the resistant flax cultivar was found to be quicker and more effective allowing translation to a higher number of activated and repressed genes in response to infection by F. oxysporum lini ( Boba et al, 2021 ). The numbers of the differentially expressed PR genes in resistant variety were higher initially (24 hpi) but similar later (48 hpi) in comparison to susceptible variety further established that the degree of the response plays deciding role in the differential resistance reaction, even though the similar qualitative response.…”

Section: Transcriptomicsmentioning

confidence: 99%

Integrated omics approaches for flax improvement under abiotic and biotic stress: Current status and future prospects

et al. 2022

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Flax (Linum usitatissimum L.) or linseed is one of the important industrial crops grown all over the world for seed oil and fiber. Besides oil and fiber, flax offers a wide range of nutritional and therapeutic applications as a feed and food source owing to high amount of α-linolenic acid (omega-3 fatty acid), lignans, protein, minerals, and vitamins. Periodic losses caused by unpredictable environmental stresses such as drought, heat, salinity-alkalinity, and diseases pose a threat to meet the rising market demand. Furthermore, these abiotic and biotic stressors have a negative impact on biological diversity and quality of oil/fiber. Therefore, understanding the interaction of genetic and environmental factors in stress tolerance mechanism and identification of underlying genes for economically important traits is critical for flax improvement and sustainability. In recent technological era, numerous omics techniques such as genomics, transcriptomics, metabolomics, proteomics, phenomics, and ionomics have evolved. The advancements in sequencing technologies accelerated development of genomic resources which facilitated finer genetic mapping, quantitative trait loci (QTL) mapping, genome-wide association studies (GWAS), and genomic selection in major cereal and oilseed crops including flax. Extensive studies in the area of genomics and transcriptomics have been conducted post flax genome sequencing. Interestingly, research has been focused more for abiotic stresses tolerance compared to disease resistance in flax through transcriptomics, while the other areas of omics such as metabolomics, proteomics, ionomics, and phenomics are in the initial stages in flax and several key questions remain unanswered. Little has been explored in the integration of omic-scale data to explain complex genetic, physiological and biochemical basis of stress tolerance in flax. In this review, the current status of various omics approaches for elucidation of molecular pathways underlying abiotic and biotic stress tolerance in flax have been presented and the importance of integrated omics technologies in future research and breeding have been emphasized to ensure sustainable yield in challenging environments.

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Transcriptomic profiling of susceptible and resistant flax seedlings after Fusarium oxysporum lini infection

Cited by 13 publications

References 45 publications

Transcriptional profiling of fusarium wilt in flax

Transcriptional profiling of fusarium wilt in flax

Genomic Regions Associated with Fusarium Wilt Resistance in Flax

Integrated omics approaches for flax improvement under abiotic and biotic stress: Current status and future prospects

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