Transcriptomic response of the Crassostrea virginica gonad after exposure to a water-accommodation fraction of hydrocarbons and the potential implications in reproduction

Tapia-Morales, Sandra; López-Landavery, Edgar A.; Giffard‐Mena, Ivone; Ramírez-Álvarez, Nancy; Gómez-Reyes, Ricardo; Dı́az, Fernando; Galindo-Sánchez, Clara E.

doi:10.1016/j.margen.2018.10.004

Cited by 7 publications

(4 citation statements)

References 74 publications

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“…This variety of functions, especially that related to the action of steroid hormones, would explain the expression levels of cyp302a1 found in La Pesca. In previous exposure results with La Pesca organisms and under laboratory conditions, we found that the presence of hydrocarbons decreased the production of steroid hormones related to reproduction compared with organisms from the same lagoon but without exposure to hydrocarbons (Tapia-Morales et al, 2019). Within the members of the superfamily of CYPs, those corresponding to groups 1 and 2 have been recognized for having a central role in the detoxification of hydrocarbons (Schlezinger et al, 2006;Zhang et al, 2012).…”

Section: Biotransformation and Excretion Of Xenobioticsmentioning

confidence: 68%

Hydrocarbons occurrence and transcriptomic response of oyster Crassostrea virginica from lagoons of the Southern Gulf of Mexico

et al. 2023

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The Gulf of Mexico is an important crude oil reserve worldwide, and the oyster Crassostrea virginica is an excellent candidate to study the oil spill impacts on marine invertebrates. In this work, the concentrations of polycyclic aromatic hydrocarbons (PAHs) and aliphatic hydrocarbons (AHs) from eight productive oyster areas in the Gulf of Mexico were measured on sediment, water, and tissues from C. virginica. In water, the highest AHs concentration was detected in Tamiahua (0.50 ng/mL), while for PAHs, the highest concentration was > 0.10 ng/mL in Tampamachoco. In sediment, Tamiahua and Tampamachoco lagoons had the highest AHs concentrations with values near 2.5 μg/g dry weight. Considering the PAHs, Tamiahua, Carmen, and Tampamachoco lagoons registered the highest levels, with values > 60 ng/g dry weight. In tissues from C. virginica, La Pesca, Cármen and Mecoacán presented the highest PAHs concentrations with values between 0.20 and 0.25 μg/g dry weight. Furthermore, from the molecular analysis of genes related with different phases of the xenobiotic detoxification process such as hypoxia inducible factor (hif-1a), cytochrome P450 10 (cyp10), flavin mono-oxygenase (fmo), glutathione S-transferase (gstΩ1), multidrug resistant protein (mdrd1), catalase (cat), among others, the differences between lagoons were significant (P< 0.05) and generally with Las Enramadas showing the highest expression levels. From genes evaluated in this study, hif-1a, cyp10, fmo, mdrd1 and cat presented the highest expression differentials between lagoons. The above allowed us to validate the utility of molecular markers in the assessment of the hydrocarbons effect on oyster under the conditions from the Southern Gulf of Mexico.

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Section: Biotransformation and Excretion Of Xenobioticsmentioning

confidence: 68%

Hydrocarbons occurrence and transcriptomic response of oyster Crassostrea virginica from lagoons of the Southern Gulf of Mexico

et al. 2023

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“…Assembled contigs completeness assessment by the representation of 978 core metazoan genes using BUSCO found 99.3% complete recovered genes for the final L. fortunei transcriptome, which is the highest percentage found for completeness of a transcriptome assembly in bivalves (47)(48)(49)(50)(51)(52)(53). Additionally, 87.94% of the clean RNA-seq reads were successfully mapped back to the assembled transcriptome, showing most of the reads being represented in the final assembly.…”

Section: A Transcriptome Assembly Quality Assessmentmentioning

confidence: 90%

Gonad transcriptome of golden musselLimnoperna fortuneireveals potential sex differentiation genes

Afonso

Americo

Souza

et al. 2019

Preprint

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The golden mussel Limnoperna fortunei is an Asian invasive bivalve that threats aquatic biodiversity and causes economic damage, especially to the hydroelectric sector in South America. Traditional control methods have been inefficient to stop the advance of the invasive mollusk, which currently is found in 40% of Brazilian hydroelectric power plants. In order to develop an effective strategy to stop golden mussel infestations, we need to better understand its reproductive and sexual mechanisms. In this study, we sequenced total RNA samples from male and female golden mussel gonads in the spawning stage. A transcriptome was assembled resulting in 200,185 contigs with 2,250 bp N50 and 99.3% completeness. Differential expression analysis identified 3,906 differentially expressed transcripts between the sexes. We searched for genes related to the sex determination/differentiation pathways in bivalves and model species and investigated their expression profiles in the transcriptome of the golden mussel gonads. From a total of 187 genes identified in the literature, 131 potential homologs were found in the L. fortunei transcriptome, of which 15 were overexpressed in males and four in females. To this group belong gene families relevant to sexual development in various organisms, from mammals to invertebrates, such as Dmrt (doublesex and mab3-related-transcription factor), Sox (SRY-related HMG-box) and Fox (forkhead box).

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“…Taxonomic and statistical analysis of samples collected during these oceanographic cruises allowed us to characterize the distribution and abundance patterns of phytoplankton (Linacre et al, 2019;Medina-Goḿez et al, 2019;Medina-Goḿez et al, 2020;Linacre et al, 2021;Ameńdola-Pimenta et al, 2021) fungi (Amend et al, 2019;Vargas-Gastélum et al, 2019), zooplankton (Hereu et al, 2021); ictyoplankton (Daudén-Bengoa et al, 2019;Daudén-Bengoa et al, 2020;Compaire et al, 2021;Santana-Cisneros et al, 2021a;Santana-Cisneros et al, 2021b), demersal fish communities in the water column (Vega Cendejas et al, 2017; Vega-Cendejas and De Santillana, 2019; Aguilar-Medrano and Vega-Cendejas, 2019a; Aguilar-Medrano and Vega-Cendejas, 2019b; Aguilar-Medrano and Vega-Cendejas, 2020b; Aguilar-Medrano and Vega-Cendejas, 2020a), benthic infauna, and invertebrates in the sediments (Hernańdez-Avila et al, 2018;Rubio et al, 2018;Aguilar-Medrano and Vega-Cendejas, 2020b;Torruco et al, 2018;Cisterna-Ceĺiz et al, 2019;Paz-Ríos and Pech, 2019;Hernández-Ávila et al, 2020;Martıńez-Aquino et al, 2020;Paz-Ríos et al, 2020;Soler-Jimenez et al, 2021;Paz-Rıós et al, 2021;Suaŕez-Mozo et al, 2021;Chí-Espínola and Vega-Cendejas, 2022;Quintanar-Retama et al, 2022). Methods for taxonomic identification ranged from the use of morphological techniques to massive sequencing of DNA molecules (metagenomics) and bioinformatic data analysis (Martínez-Aquino et al, 2017;Puch-Hau et al, 2018a;Escobar-Zepeda et al, 2018;Batta-Lona et al, 2019;Tapia-Morales et al, 2019;Vargas-Gastélum et al, ...…”

Section: Baseline Studies and Environmental Variabilitymentioning

confidence: 99%

Ocean monitoring, observation network and modelling of the Gulf of Mexico by CIGOM

et al. 2023

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The tragic accident of the Macondo platform operated by British Petroleum (BP) unleashed in 2010 one of the largest oil spills in history, lasting over three months, spilling nearly 500 million liters of oil in one of the most biodiverse ocean regions. This accident revealed the technological deficiencies for the control of a spill in deep waters of the hydrocarbon industry. Simultaneously it showed important gaps in knowledge to predict the propagation and fate of the large volumes of hydrocarbons at depth and on the surface ocean and, more importantly, on their impact on the great ecosystem of the Gulf of Mexico. The necessity to understand and predict the transport, fate and ecosystem-level impacts of large oil spills in the southern Gulf of Mexico, a key region for oil exploration and extraction, led policymakers, scientists, and industry representatives from PEMEX (the Mexican oil company) to jointly launch an ocean observation project (2015-22) aimed to provide a multi-layered environmental baseline, develop a modern monitoring and computational modeling capacity and promote scientific understanding of the marine environment throughout the Mexican Exclusive Economic Zone (EEZ). The initiative, led by the Research Consortium for the Gulf of Mexico (CIGoM), brought together more than 300 multidisciplinary researchers from more than a two dozen institutions in Mexico and abroad, including the Centre for Scientific Research and Higher Education of Ensenada (CICESE) as the leading institution, the National Autonomous University of Mexico (UNAM), the Centre for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV) in Mérida, the Autonomous University of Baja California (UABC), and the Centre for Engineering and Industrial Development (CIDESI). Financial support was provided by the National Council for Science and Technology and the Ministry of Energy Hydrocarbon Fund.

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Transcriptomic response of the Crassostrea virginica gonad after exposure to a water-accommodation fraction of hydrocarbons and the potential implications in reproduction

Cited by 7 publications

References 74 publications

Hydrocarbons occurrence and transcriptomic response of oyster Crassostrea virginica from lagoons of the Southern Gulf of Mexico

Hydrocarbons occurrence and transcriptomic response of oyster Crassostrea virginica from lagoons of the Southern Gulf of Mexico

Gonad transcriptome of golden musselLimnoperna fortuneireveals potential sex differentiation genes

Ocean monitoring, observation network and modelling of the Gulf of Mexico by CIGOM

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