Transcriptomic response to cold of thermophilous medicinal plant Marsdenia tenacissima

Long, Guangqiang; Zhao, Changhong; Zhao, Ping; Zhou, Changjian; Elie, Ntirenganya; Zhou, Yanli

doi:10.1016/j.gene.2020.144602

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…As we previously found cis ‐elements such as MYB and MYC in the cloned promoter of a polyoxypregnane glycosides pathway gene (Long et al., 2020), we investigated the effects of Ca 2+ on tenacissoside secondary metabolites. High‐performance liquid chromatograph (HPLC) analysis revealed that Ca 2+ treatment increased the contents of tenacissoside I, G and H (Figure 6), suggesting that these active metabolites result from genomic adaptation to karst calcium levels.…”

Section: Resultsmentioning

confidence: 99%

Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis

et al. 2023

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SUMMARY Marsdenia tenacissima is a medicinal plant widely distributed in the calcium‐rich karst regions of southwest China. However, the lack of a reference genome has hampered the implementation of molecular techniques in its breeding, pharmacology and domestication. We generated the chromosome‐level genome assembly in Apocynaceae using combined SMRT sequencing and Hi‐C. The genome length was 381.76 Mb, with 98.9% of it found on 11 chromosomes. The genome contained 222.63 Mb of repetitive sequences and 21 899 predicted gene models, with a contig N50 of 6.57 Mb. Phylogenetic analysis revealed that M. tenacissima diverged from Calotropis gigantea at least 13.43 million years ago. Comparative genomics showed that M. tenacissima underwent ancient shared whole‐genome duplication. This event, together with tandem duplication, contributed to 70.71% of gene‐family expansion. Both pseudogene analysis and selective pressure calculations suggested calcium‐related adaptive evolution in the M. tenacissima genome. Calcium‐induced differentially expressed genes (DEGs) were mainly enriched in cell‐wall‐related processes. Domains (e.g. Fasciclin and Amb_all) and cis‐elements (e.g. MYB and MYC) frequently occurred in the coding and promoter regions of cell‐wall DEGs, respectively, and the expression levels of these genes correlated significantly with those of calcium‐signal‐related transcription factors. Moreover, calcium addition increased tenacissoside I, G and H contents. The availability of this high‐quality genome provides valuable genomic information for genetic breeding and molecular design, and lends insights into the calcium adaptation of M. tenacissima in karst areas.

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Section: Resultsmentioning

confidence: 99%

Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis

et al. 2023

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“… 19 TEH, along with the other 2 ingredients (tenacissoside I and G), is extracted form Marsdenia tenacissima and has been found with anti-inflammatory and anti-tumor effects. 20 Interestingly, a recent study proved that the Marsdenia tenacissima extract (MTE) promotes gefitinib accumulation in lung cancer tissues via fasciliating the activities of ABCG2, which is the primary drug transporter for gefitinib. 21 Our study found that with the increase of TEH concentration, the proliferative ability of HCC cells decreased and cell apoptosis increased.…”

Section: Discussionmentioning

confidence: 99%

Tenacissoside H Induces Autophagy and Radiosensitivity of Hepatocellular Carcinoma Cells by PI3K/Akt/mTOR Signaling Pathway

et al. 2021

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Tenacissoside H (TEH), which has anti-inflammatory and anti-tumor effects, is a major active ingredient extracted from the stem of Marsdenia tenacissima. However, the effect of TEH on hepatocellular carcinoma (HCC) as well as the underlying mechanisms are still indistinct. Presently, HCC cells (including Huh-7 and HepG2) were dealt with different concentrations of TEH. The proliferation and apoptosis of HCC cells were determined via Cell Counting Kit-8 (CCK8) assay and flow cytometry. In addition, Western blot was conducted to evaluate the expressions of autophagy—and apoptosis-related proteins. Tissue immunofluorescence was carried out to evaluate LC3B expression in the tumor tissues. The data showed that TEH suppressed the growth of HCC cells in a concentration-dependent manner. Besides, TEH enhanced radiosensitivity and promoted the apoptosis of HCC cells. Moreover, the mRNA and protein levels of autophagy-related genes (LC3-II/LC2-I, ATG5, Beclin-1) were significantly promoted by TEH. Mechanistically, TEH attenuated the activation of PI3K/Akt/mTOR signaling pathway. However, inhibition of PI3 K pathway abolished the anti-tumor effects of TEH in HCC cells. Collectively, this study suggested that TEH increases the radiosensitivity of HCC cells via inducing autophagy and apoptosis through downregulating PI3K/Akt/mTOR signaling pathway.

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“…M. tenacissima has antitussive, antihypertensive, heat-clearing, and anti-inflammatory effects [18] and can effectively suppress malignant tumors [19,20]; its medicinal value has always been a primary research object. The 'Xiao-ai-ping' agent made from M. tenacissima received extensive attention after entering the market [21]. Many of the important pharmacological active ingredients of medicinal plants are secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Light Intensity on the Chlorogenic Acid Biosynthesis Pathway in Marsdenia tenacissima

Meng,

Li,

et al. 2024

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The goal of this study was to understand the effect of light intensity on the chlorogenic acid content and biosynthesis-related gene expression in Marsdenia tenacissima. In this study, M. tenacissima plants were treated with different light intensities; the chlorogenic acid content was determined by high-performance liquid chromatography; and transcriptome sequencing was performed. The amount of chlorogenic acid in the control was the highest and differed significantly from that under three different shading treatments. With a decrease in light intensity, the content of chlorogenic acid also showed a decreasing trend. A total of 1149 differentially expressed genes were identified by transcriptome sequencing, and most of the genes were down-regulated under the 90% shading treatment. A weighted gene co-expression network analysis identified the differentially expressed genes associated with light-induced chlorogenic acid biosynthesis. The different shading treatments down-regulated the expression of the chlorogenic acid biosynthesis pathway structural genes (HCTs). The MIKC family genes were the main transcription factors regulating light-induced chlorogenic acid biosynthesis, but the MYB and SBP family genes were also involved. In summary, combined physiological and transcriptome analysis, candidate structural genes, and transcription factors in the biosynthesis pathway of chlorogenic acid were identified in M. tenacissima.

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Transcriptomic response to cold of thermophilous medicinal plant Marsdenia tenacissima

Cited by 9 publications

References 22 publications

Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis

Marsdenia tenacissima genome reveals calcium adaptation and tenacissoside biosynthesis

Tenacissoside H Induces Autophagy and Radiosensitivity of Hepatocellular Carcinoma Cells by PI3K/Akt/mTOR Signaling Pathway

The Effect of Light Intensity on the Chlorogenic Acid Biosynthesis Pathway in Marsdenia tenacissima

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