Transcriptomic response to low salinity stress in gills of the Pacific white shrimp, Litopenaeus vannamei

Hu, Dongxu; Pan, Luqing; Zhao, Qun; Ren, Qi

doi:10.1016/j.margen.2015.07.003

Cited by 47 publications

(25 citation statements)

References 91 publications

(92 reference statements)

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“…N'Golo et al reported the changes of ionocyte morphology and function after transfer from fresh to hypersaline waters in the tilapia Sarotherodon melanotheron [39]. High-throughput RNA-seq is a good method in illuminating the underlying molecular mechanisms of osmoregulation and immunization [40,41], and the transcriptomic analysis with osmotic stress has been realized in some aquatic vertebrate species and invertebrate species, such as Oreochromis mossambicus [42], Acipenser baeri [35], Oryzias latipes [43], Litopenaeus vannamei [13,31], Eriocheir sinensis [32], Crassostrea gigas [44] and Mytilus trossulus [34]. However, most of these studies were directed to only one osmotic stress treatment, but the natural water environment is complex and changing, so it is important to perform various osmotic stress treatments on fish.…”

Section: Discussionmentioning

confidence: 99%

“…Carbonate alkalinity stress was considered as a major risk factor for fishes surviving in saline-alkaline water [6,7]. Although many studies have examined osmoregulatory physiology in freshwater and marine populations of fish [8][9][10][11][12], it still remains unknown about the molecular mechanism of osmotic stress tolerance [13]. Therefore, the identification and characterization of the genes and the regulatory factors involved in hyperosmoregulation are now essential for increasing the production and the efficiency of selective breeding programs for some important fish species.…”

Section: Introductionmentioning

confidence: 99%

“…Recent years, with the prosperous development of the next-generation sequencing, high-throughput sequencing has been widely used to identify conserved and novel functional genes and signaling pathways in aquatic animals, such as Litopenaeus vannamei [13,31], Eriocheir sinensis [32], Palaemon caridean shrimps [33] and Mytilus trossulus [34]. Guo et al identified three possible osmoregulation-related signaling pathways as lipid metabolism related pathways, tight junction pathway and thyroid hormone signaling pathway in Siberian sturgeon Acipenser baeri in response to salinity stress [35].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male)

Zhu

et al. 2020

BMC Genomics

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Background: Osmotic stress is a widespread phenomenon in aquatic animal. The ability to cope with salinity stress and alkaline stress is quite important for the survival of aquatic species under natural conditions. Tilapia is an important commercial euryhaline fish species. What's more tilapia is a good experimental material for osmotic stress regulation research, but the molecular regulation mechanism underlying different osmotic pressure of tilapia is still unexplored. Results: To elucidate the osmoregulation strategy behind its hyper salinity, alkalinity and salinity-alkalinity stress of tilapia, the transcriptomes of gills in hybrid tilapia (Oreochromis mossambicus ♀ × O. urolepis hornorum ♂) under salinity stress (S: 25‰), alkalinity stress(A: 4‰) and salinity-alkalinity stress (SA: S: 15‰, A: 4‰) were sequenced using deep-sequencing platform Illumina/HiSeq-2000 and differential expression genes (DEGs) were identified. A total of 1958, 1472 and 1315 upregulated and 1824, 1940 and 1735 downregulated genes (P-value < 0.05) were identified in the salt stress, alkali stress and saline-alkali stress groups, respectively, compared with those in the control group. Furthermore, Kyoto Encyclopedia of Genes and Genomes pathway analyses were conducted in the significant different expression genes. In all significant DEGs, some of the typical genes involved in osmoregulation, including carbonic anhydrase (CA), calcium/calmodulin-dependent protein kinase (CaM kinase) II (CAMK2), aquaporin-1(AQP1), sodium bicarbonate cotransporter (SLC4A4/NBC1), chloride channel 2(CLCN2), sodium/ potassium/chloride transporter (SLC12A2 / NKCC1) and other osmoregulation genes were also identified. RNA-seq results were validated with quantitative real-time PCR (qPCR), the 17 random selected genes showed a consistent direction in both RNA-Seq and qPCR analysis, demonstrated that the results of RNA-seq were reliable. Conclusions:The present results would be helpful to elucidate the osmoregulation mechanism of aquatic animals adapting to saline-alkali challenge. This study provides a global overview of gene expression patterns and pathways that related to osmoregulation in hybrid tilapia, and could contribute to a better understanding of the molecular regulation mechanism in different osmotic stresses.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male)

Zhu

et al. 2020

BMC Genomics

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“…As one of the non‐protein free amino acids, gamma‐aminobutyric acid (GABA) was first synthesized in 1883 and it is wildly known as a neurotransmitter of the nervous system. It is also related to abiotic resistances such as salinity stress in crustaceans (Barman et al, ; Carmichael et al, ; Chen, Chen, et al, ; Chen, Li, et al, ; Hu, Pan, Zhao, & Ren, ).…”

Section: Introductionmentioning

confidence: 99%

Long‐term low‐salinity stress affects growth, survival and osmotic related gamma‐aminobutyric acid regulation in the swimming crab Portunus trituberculatus

Zheng

et al. 2019

Aquac Res

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A 30‐day experiment was conducted to investigate the effects of long‐term low‐salinity stress on the growth performance and osmotic related chlorine ion channel ligand regulation: gamma‐aminobutyric acid (GABA) content and GABAA receptor‐associated protein (GABARAP) expression in Portunus trituberculatus. The salinity levels of both the control group and the experimental group were 30 and 12 psu respectively. After rearing for 30 days, the specific growth rate and survival rate were compared between the two groups, and salinity 6 psu was used to test the salinity tolerance. The results were as follows: (a) Both the specific growth rate and survival rate were significant lower in the experimental group (p < 0.05) after 30 days; (b) After challenge with salinity 6 psu for 72 hr, the crabs of experimental group had a 100% survival rate, whereas the crabs of the control group were all dead within 48 hr; (c) The content of GABA and the gene expression level of GABARAP in experimental group were significant different from control group (p < 0.05) after challenge via salinity 6 psu. In the control group, the GABA content increased rapidly from 9.96 ± 2.09 to 42.00 ± 5.94 µg/g; however, in the experimental group, it only increased to 27.82 ± 2.55 µg/g; the gene expression of GABARAP in the experimental group increased to the maximum at 24 hr, then decreased and stabilized at 48 hr, suggesting that GABA and GABARAP were trigged during the early stage of low‐salinity stress resistance.

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“…Salinity is an important environmental factor of osmotic pressure which is closely related to aquatic organisms for survival and evolve. The physiological reactions and tolerance against changes of salinity have been reported in several aquatic animals (Blewett et al, 2015;Berg et al, 2015;Hu et al, 2015;Nikapitiya et al, 2015). Salinity changes often cause stress reaction of marine organisms, and make the body produce excess reactive oxygen species which do harm to the body.…”

Section: Introductionmentioning

confidence: 99%

Effects of Acute Low-Salinity Stress on the Activities of Catalase (CAT), Superoxide Dismutase (SOD) and Glutathiones-Transferase (GST) in Scapharca broughtonii

Wu¹,

Yang²,

Yan³

et al. 2019

JMBR

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Salinity is an important environmental factor of osmotic pressure which is closely related to growth and development of aquatic organisms. Some physiological studies regarding to challenge of salinity have been reported in marine shellfish. However, information about the effects of acute low-salinity stress on the enzyme activities in ark shell, Scapharca broughtonii, is still limited now. In this study, the enzyme activities of catalase (CAT), superoxide dismutase (SOD) and glutathiones-transferase (GST) in five tested tissues of S. broughtonii under different salinity (10, 15, 20, 25 and 30&permil;) for different challenged time (24, 48, 72 and 96h) were investigated. The dynamic change of the three enzyme activities after challenged were further analyzed according to the detected data. Results revealed that low-salinity stress generated great change of CAT, SOD and GST activities in organism, but the change peculiarity in different tissues was different in some degree. Especially, two of the five tissues, hepatopancreas and foot, showed special characteristics responding to the low-salinity stress. It also showed that the variations of the three enzymes activities have tissue specificity and time sequentiality in S. broughtonii.

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Transcriptomic response to low salinity stress in gills of the Pacific white shrimp, Litopenaeus vannamei

Cited by 47 publications

References 91 publications

Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male)

Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male)

Long‐term low‐salinity stress affects growth, survival and osmotic related gamma‐aminobutyric acid regulation in the swimming crab Portunus trituberculatus

Effects of Acute Low-Salinity Stress on the Activities of Catalase (CAT), Superoxide Dismutase (SOD) and Glutathiones-Transferase (GST) in Scapharca broughtonii

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