Transcriptomic responses to drought stress among natural populations provide insights into local adaptation of weeping forsythia

Li, Yong; Shi, Long-Chen; Pei, Nancai; Cushman, Samuel A.; Si, Yu-Tao

doi:10.1186/s12870-021-03075-6

Cited by 9 publications

(7 citation statements)

References 49 publications

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“…Therefore, the gene expression data in leaves was used for analysis in our study. Previous studies ( Li et al, 2021a , b ) showed that Wuzhishan population has the highest cold and drought tolerance when compared with the other three populations. Thus, the gene expression data of the Wuzhishan population was used as a representative.…”

Section: Methodsmentioning

confidence: 83%

“…Expression patterns of the CCD genes in F. suspensa under drought stress were from Wuzhishan population under 80 and 20% soil water content (accession no. SRX7503009 , SRX7503010 , SRX7503012 - SRX7503015 ; Li et al, 2021a ). Expression patterns of the CCD genes in F. suspensa under cold stress were from Wuzhishan populations at 25 and 4°C (accession no.…”

Section: Methodsmentioning

confidence: 99%

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Genome-wide analysis of the carotenoid cleavage dioxygenases gene family in Forsythia suspensa: Expression profile and cold and drought stress responses

et al. 2022

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Forsythia suspensa is a famous ornamental and medicinal plant in Oleaceae. CCD family is involved in the synthesis of pigments, volatiles, strigolactones, and abscisic acid (ABA) in plants. In this study, the CCD family in F. suspensa was analyzed at the genome level. A total of 16 members of the CCD family were identified, which included 11 members of the carotenoid cleavage dioxygenases (CCD) subfamily and 5 members of the 9-cis epoxycarotenoid dioxygenases (NCED) subfamily. The expression analysis of different tissues demonstrated that three FsCCD1 genes might be involved in the synthesis of pigments and volatiles in flowers and fruits. Three CCD4 genes were effectively expressed in flowers, while only FsCCD4-3 was effectively expressed in fruits. Comparison of CCD4 between Osmanthus fragrans and F. suspensa showed that the structure of FsCCD4-1 is was comparable that of OfCCD4-1 protein, indicating that the protein might be performing, especially in catalyzing the synthesis of β-ionone. However, further comparison of the upstream promoter regions showed that the proteins have major differences in the composition of cis-elements, which might be responsible for differences in β-ionone content. On the other hand, four NCED genes were significantly up-regulated under cold stress while two were up-regulated in drought stress. The data showed that these genes might be involved in the synthesis of ABA. Taken together, our data improves understanding of the CCD family and provides key candidate genes associated with cold and drought stresses in F. suspensa.

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Section: Methodsmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Genome-wide analysis of the carotenoid cleavage dioxygenases gene family in Forsythia suspensa: Expression profile and cold and drought stress responses

et al. 2022

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“…The gene expression data of weeping forsythia under drought stress were from Wuzhishan populations under 80% and 20% soil water content (accession no. SRX7503009, SRX7503010, SRX7503012-SRX7503015; Li et al 2021b ). The gene expression data of weeping forsythia under cold stress were from Wuzhishan populations at 25 ℃ and 4 ℃ (accession no.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide investigation of the WRKY transcription factor gene family in weeping forsythia: expression profile and cold and drought stress responses

et al. 2023

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Weeping forsythia is a wide-spread shrub in China with important ornamental, medicinal and ecological values. It is widely distributed in China's warm temperate zone. In plants, WRKY transcription factors play important regulatory roles in seed germination, flower development, fruit ripening and coloring, and biotic and abiotic stress response. To date, WRKY transcription factors have not been systematically studied in weeping forsythia. In this study, we identified 79 WRKY genes in weeping forsythia and classified them according to their naming rules in Arabidopsis thaliana. Phylogenetic tree analysis showed that, except for IIe subfamily, whose clustering was inconsistent with A. thaliana clustering, other subfamily clustering groups were consistent. Cis-element analysis showed that WRKY genes related to pathogen resistance in weeping forsythia might be related to methyl jasmonate and salicylic acid-mediated signaling pathways. Combining cis-element and expression pattern analyses of WRKY genes showed that more than half of WRKY genes were involved in light-dependent development and morphogenesis in different tissues. The gene expression results showed that 13 WRKY genes were involved in drought response, most of which might be related to the abscisic acid signaling pathway, and a few of which might be regulated by MYB transcription factors. The gene expression results under cold stress showed that 17 WRKY genes were involved in low temperature response, and 9 of them had low temperature responsiveness cis-elements. Our study of WRKY family in weeping forsythia provided useful resources for molecular breeding and important clues for their functional verification.

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“…The DEGs of all populations studied largely comprise photosynthesis, oxidation-reduction, and cell membrane component-related genes (Li et al, 2021). Populations of Eucalyptus grandis (Myrtaceae)…”

Section: Transcriptomic Plasticity Drives Differential Drought Responsesmentioning

confidence: 99%

A genotype × environment experiment reveals contrasting response strategies to drought between populations of a keystone species (Artemisia tridentata; Asteraceae)

Melton,

Moran,

Martinez

et al. 2023

Plant Enviro Interactions

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Western North America has been experiencing persistent drought exacerbated by climate change for over two decades. This extreme climate event is a clear threat to native plant communities. Artemisia tridentata is a keystone shrub species in western North America and is threatened by climate change, urbanization, and wildfire. A drought Genotype × Environment (G × E) experiment was conducted to assess phenotypic plasticity and differential gene expression in A. tridentata. The G × E experiment was performed on diploid A. tridentata seedlings from two populations (one from Idaho, USA and one from Utah, USA), which experience differing levels of drought stress during the summer months. Photosynthetic data, leaf temperature, and gene expression levels were compared between treatments and populations. The Utah population maintained higher photosynthetic rates and photosynthetic efficiency than the Idaho population under drought stress. The Utah population also exhibited far greater transcriptional plasticity than the Idaho population and expressed genes of response pathways distinct from those of the Idaho population. Populations of A. tridentata differ greatly in their drought response pathways, likely due to differences in response pathways that have evolved under distinct climatic regimes. Epigenetic processes likely contribute to the observed differences between the populations.

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Transcriptomic responses to drought stress among natural populations provide insights into local adaptation of weeping forsythia

Cited by 9 publications

References 49 publications

Genome-wide analysis of the carotenoid cleavage dioxygenases gene family in Forsythia suspensa: Expression profile and cold and drought stress responses

Genome-wide analysis of the carotenoid cleavage dioxygenases gene family in Forsythia suspensa: Expression profile and cold and drought stress responses

Genome-wide investigation of the WRKY transcription factor gene family in weeping forsythia: expression profile and cold and drought stress responses

A genotype × environment experiment reveals contrasting response strategies to drought between populations of a keystone species (Artemisia tridentata; Asteraceae)

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