Transcriptomic Study of Porcine Small Intestine Epithelial Cells Reveals Important Genes and Pathways Associated With Susceptibility to Escherichia coli F4ac Diarrhea

Augustino, Serafino M. A.; Xu, Qiang; Liu, Xueqin; Liu, Lei; Zhang, Qin; Yu, Ying

doi:10.3389/fgene.2020.00068

Cited by 9 publications

(7 citation statements)

References 39 publications

(49 reference statements)

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“…The versatility of KCNMA1 due to multiple Ca 2+ perception sites explains the presence of these channels in different human cell types, in which Ca 2+ concentrations can vary widely [ 38 ]. In a recent transcriptome study of porcine small intestinal epithelial cells obtained from tissue samples of large white piglets, upregulation of KCNMA1 was observed in pigs with a phenotype susceptible to enterotoxigenic Escherichia coli (ETEC) F4 compared with naturally resistant pigs [ 39 ]. Pigs susceptible to ETEC express higher levels of KCNMA1 in epithelial cells of the small intestine than pigs with a naturally resistant phenotype, which is due to a functional ETEC F4 receptor [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…In a recent transcriptome study of porcine small intestinal epithelial cells obtained from tissue samples of large white piglets, upregulation of KCNMA1 was observed in pigs with a phenotype susceptible to enterotoxigenic Escherichia coli (ETEC) F4 compared with naturally resistant pigs [ 39 ]. Pigs susceptible to ETEC express higher levels of KCNMA1 in epithelial cells of the small intestine than pigs with a naturally resistant phenotype, which is due to a functional ETEC F4 receptor [ 39 ]. Another transcriptome study on human PBMC described that KCNMA1 is upregulated in PBMC from patients with type 1 diabetes mellitus compared with a healthy control group [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

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Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome

et al. 2022

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Bovine paratuberculosis is a serious chronic disease of the gastrointestinal tract that causes economic losses and dramatically affects animal health in livestock. The underlying infectious agent, Mycobacterium avium subspecies paratuberculosis (MAP), cannot reliably be detected by standard diagnostic tests due to the long asymptomatic disease stage. The aim of this study was to detect proteomic changes in peripheral blood mononuclear cells (PBMC) from cows of the same herd with different MAP infection status after co-incubation with viable MAP in vitro using label-free LC-MS/MS. In our proteomic discovery experiment, we detected 2631 differentially regulated proteins between cows with negative MAP infection status (so-called MAP-resistant cows) and cows with positive MAP infection status (so-called persistently MAP-infected cows). In MAP-resistant cows, we detected enriched immune-related signaling pathways for TLR2 and MHC class II component proteins, among others, indicating a successful defensive immune response of the cows to MAP. In contrast, persistently MAP-infected cows were not directly enriched in immune-related signaling pathways associated with ITGA2B and KCNMA1, among others. The introduction of these distinct immune responses contributes to a better understanding of the bovine immune response and mechanisms of susceptibility to MAP.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome

et al. 2022

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“…Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. More details about library preparation have been described in our previously published paper [ 61 ]. The libraries were sequenced on an Illumina platform (HiSeq Xten) and 150 bp paired-end reads were generated.…”

Section: Methodsmentioning

confidence: 99%

“…Combining our previous mRNA data (PRJNA562774, more details can be found in previously published paper [ 61 ].) with predicted DE circRNA targeted miRNA data, we constructed a regulatory network of circRNA-miRNA-mRNA to reveal the potential association of ETEC-F4ac adhesion in small intestinal epithelial cells of piglets.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive analysis of circular RNAs in porcine small intestine epithelial cells associated with susceptibility to Escherichia coli F4ac diarrhea

Zhao

Serafino

et al. 2023

BMC Genomics

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Background Diarrhea is one of the most common diseases in pig industry, which seriously threatens the health of piglets and causes huge economic losses. Enterotoxigenic Escherichia coli (ETEC) F4 is regarded as the most important cause of diarrhea in piglets. Some pigs are naturally resistant to those diarrheas caused by ETEC-F4, because they have no F4 receptors (F4R) on their small intestine epithelial cells that allow F4 fimbriae adhesion. Circular RNA (circRNA) has been shown to play an important regulatory role in the pathogenesis of disease. We hypothesized that circRNAs may also regulate the adhesion of piglet small intestinal epithelial cells to ETEC F4 fimbriae. However, the circRNA expression profiles of piglets with different Enterotoxigenic Escherichia coli F4 fimbriae (ETEC-F4ac) adhesion phenotypes are still unclear, and the intermediate regulatory mechanisms need to be explored. Hence, the present study assessed the circRNA expression profiling in small intestine epithelial cells of eight male piglets with different ETEC-F4 adhesion phenotypes and ITGB5 genotypes to unravel their regulatory function in susceptibility to ETEC-F4ac diarrhea. Piglets were divided into two groups: non-adhesive group (n = 4) with CC genotype and adhesive group (n = 4) with TT genotype. Results The RNA-seq data analysis identified 13,199 circRNAs from eight samples, most of which were exon-derived. In the small intestine epithelial cells, 305 were differentially expressed (DE) circRNAs between the adhesive and non-adhesive groups; of which 46 circRNAs were upregulated, and 259 were downregulated. Gene ontology and KEGG enrichment analysis revealed that most significantly enriched DE circRNAs’ host genes were linked to cytoskeletal components, protein phosphorylation, cell adhesion, ion transport and pathways (such as adherens junction, gap junction) associated with ETEC diarrhea. The circRNA-miRNA-mRNA interaction network was also constructed to elucidate their underlying regulatory relationships. Our results identified several candidate circRNAs that affects susceptibility to ETEC diarrhea. Among them, circ-SORBS1 can adsorb ssc-miR-345-3p to regulate the expression of its host gene SORBS1, thus improving cell adhesion. Conclusion Our results provided insights into the regulation function of circRNAs in susceptibility to ETEC diarrhea of piglets, and enhanced our understanding of the role of circRNAs in regulating ETEC diarrhea, and reveal the great potential of circRNA as a diagnostic marker for susceptibility of ETEC diarrhea in piglets.

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“…This failure of the pathogen to attach or adhere to the receptor stops the very first step of host–pathogen interaction and prevents infection or transfer of toxic compounds (Plastow 2016 ). Two examples of such genetic resistance in pigs are related to scours caused by E. coli F18 and F4 (or K88) (Augustino et al 2020 ; Bao et al 2012 ; Fu et al 2012 ; Meijerink et al 2000 ; Meijerink et al 1997 ; Ren et al 2012 ; Zhang et al 2008 ). Such resistance is the most cost-effective mechanism of preventing infection from the host perspective as there is no need to increase energy expenditure on the immune system for defending against infectious agents when the resistant animal can avoid infection and maintain a healthy status.…”

Section: Main Textmentioning

confidence: 99%

Breeding for disease resilience: opportunities to manage polymicrobial challenge and improve commercial performance in the pig industry

Bai

Plastow

2022

CABI Agric Biosci

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Disease resilience, defined as an animal’s ability to maintain productive performance in the face of infection, provides opportunities to manage the polymicrobial challenge common in pig production. Disease resilience can deliver a number of benefits, including more sustainable production as well as improved animal health and the potential for reduced antimicrobial use. However, little progress has been made to date in the application of disease resilience in breeding programs due to a number of factors, including (1) confusion around definitions of disease resilience and its component traits disease resistance and tolerance, and (2) the difficulty in characterizing such a complex trait consisting of multiple biological functions and dynamic elements of rates of response and recovery from infection. Accordingly, this review refines the definitions of disease resistance, tolerance, and resilience based on previous studies to help improve the understanding and application of these breeding goals and traits under different scenarios. We also describe and summarize results from a “natural disease challenge model” designed to provide inputs for selection of disease resilience. The next steps for managing polymicrobial challenges faced by the pig industry will include the development of large-scale multi-omics data, new phenotyping technologies, and mathematical and statistical methods adapted to these data. Genome editing to produce pigs resistant to major diseases may complement selection for disease resilience along with continued efforts in the more traditional areas of biosecurity, vaccination and treatment. Altogether genomic approaches provide exciting opportunities for the pig industry to overcome the challenges provided by hard-to-manage diseases as well as new environmental challenges associated with climate change.

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Transcriptomic Study of Porcine Small Intestine Epithelial Cells Reveals Important Genes and Pathways Associated With Susceptibility to Escherichia coli F4ac Diarrhea

Cited by 9 publications

References 39 publications

Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome

Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome

Comprehensive analysis of circular RNAs in porcine small intestine epithelial cells associated with susceptibility to Escherichia coli F4ac diarrhea

Breeding for disease resilience: opportunities to manage polymicrobial challenge and improve commercial performance in the pig industry

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