Transcriptomics and coexpression network profiling of the effects of levamisole hydrochloride on Bursaphelenchus xylophilus

Chen, Jie; Hao, Xin; Wang, Buyong; Ma, Ling

doi:10.1016/j.pestbp.2021.105019

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…The sensitivity analysis of Bx-cyp29A3 to different concentrations of L(−)-carvone provided an effective theoretical basis to reveal the metabolic mechanism and control measures for the detoxification of PWNs. Prior studies have proven that cytochrome P450 plays a crucial role in the low-temperature resistance, vitality, dispersal ability, reproduction, pathogenicity, and pesticide metabolism of PWNs [ 40 , 41 , 64 , 65 ]. However, to date, the mechanism of cytochrome P450 in the plant secondary metabolite resistance to PWNs remains unclear.…”

Section: Discussionmentioning

confidence: 99%

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Functional Study on Cytochrome P450 in Response to L(−)-Carvone Stress in Bursaphelenchus xylophilus

Chen

Hao

Tan

et al. 2022

Genes

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Bursaphelenchus xylophilus (PWN) causes pine wilt disease (PWD), which is one of the most devastating pine diseases worldwide. Cytochrome P450 (CYP) catalyzes the biosynthetic metabolism of terpenoids and plays an important role in the modification of secondary metabolites in all living organisms. We investigated the molecular characteristics and biological functions of Bx-cyp29A3 in B. xylophilus. The bioinformatics analysis results indicated that Bx-cyp29A3 has a transmembrane domain and could dock with L(−)-carvone. The gene expression pattern indicated that Bx-cyp29A3 was expressed in 0.2, 0.4, 0.6, 0.8, and 1.0 mg/mL L(−)-carvone solutions. The Bx-cyp29A3 expression increased in a dose-dependent manner and peaked at 24 h of exposure when the L(−)-carvone solution concentration was 0.8 mg/mL. However, the gene expression peaked at 0.6 mg/mL after 36 h. Furthermore, RNA interference (RNAi) indicated that Bx-cyp29A3 played an essential role in the response to L(−)-carvone. The mortality rates of the Bx-cyp29A3 knockdown groups were higher than those of the control groups in the 0.4, 0.6, 0.8, and 1.0 mg/mL carvone solutions after 24 h of exposure or 36 h of exposure. In summary, bioinformatics provided the structural characteristics and conserved sequence properties of Bx-cyp29A3 and its encoded protein, which provided a target gene for the study of the P450 family of B. xylophilus. Gene silencing experiments clarified the function of Bx-cyp29A3 in the immune defense of B. xylophilus. This study provides a basis for the screening of new molecular targets for the prevention and management of B. xylophilus.

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Section: Discussionmentioning

confidence: 99%

“…With the development of bioinformatics research, greater progress has been made in the functional study of cytochrome P450 in the nematode. The CyP450 gene responds to β-pinene [ 39 ] and levamisole hydrochloride [ 40 ] stress in pine wood nematodes. CyP33C9 affects the reproduction and pathogenicity of pine wood nematodes [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Study on Cytochrome P450 in Response to L(−)-Carvone Stress in Bursaphelenchus xylophilus

Chen

Hao

Tan

et al. 2022

Genes

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“…The primer sequences for target genes are outlined in Table 4 . All mRNA levels were normalized by the Actin level [ 49 ]. Gene expression was relatively quantitated by the 2 −ΔΔCT method.…”

Section: Methodsmentioning

confidence: 99%

Discovery of 1,2,4-Oxadiazole Derivatives Containing Haloalkyl as Potential Acetylcholine Receptor Nematicides

Luo

Zhang

et al. 2023

IJMS

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Plant-parasitic nematodes pose a serious threat to crops and cause substantial financial losses due to control difficulties. Tioxazafen (3-phenyl-5-thiophen-2-yl-1,2,4-oxadiazole) is a novel broad-spectrum nematicide developed by the Monsanto Company, which shows good prevention effects on many kinds of nematodes. To discover compounds with high nematocidal activities, 48 derivatives of 1,2,4-oxadiazole were obtained by introducing haloalkyl at the 5-position of tioxazafen, and their nematocidal activities were systematically evaluated. The bioassays revealed that most of 1,2,4-oxadiazole derivatives showed remarkable nematocidal activities against Bursaphelenchus xylophilus, Aphelenchoides besseyi, and Ditylenchus dipsaci. Notably, compound A1 showed excellent nematocidal activity against B. xylophilus with LC50 values of 2.4 μg/mL, which was superior to that of avermectin (335.5 μg/mL), tioxazafen (>300 μg/mL), and fosthiazate (436.9 μg/mL). The transcriptome and enzyme activity results indicate that the nematocidal activity of compound A1 was mainly related to the compound which affected the acetylcholine receptor of B. xylophilus.

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Assessing effect of Trichoderma asperellum T16 on management of Bursaphelenchus xylophilus

Chen,

Jiao,

Ran

et al. 2024

Industrial Crops and Products

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Transcriptomics and coexpression network profiling of the effects of levamisole hydrochloride on Bursaphelenchus xylophilus

Cited by 5 publications

References 45 publications

Functional Study on Cytochrome P450 in Response to L(−)-Carvone Stress in Bursaphelenchus xylophilus

Functional Study on Cytochrome P450 in Response to L(−)-Carvone Stress in Bursaphelenchus xylophilus

Discovery of 1,2,4-Oxadiazole Derivatives Containing Haloalkyl as Potential Acetylcholine Receptor Nematicides

Assessing effect of Trichoderma asperellum T16 on management of Bursaphelenchus xylophilus

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