Transcriptomics and metabolomics analyses reveal the differential accumulation of phenylpropanoids between Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu

Gao, Hongyang; Xu, Danyun; Zhang, Huiju; Qian, Jia-Ping; Yang, Quan

doi:10.1016/j.indcrop.2020.112282

Cited by 17 publications

(18 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TOF mass arrangement was 50-1200 Da and the scan time was 0.2 s. For MS/MS detection, all precursors were fragmented using 20-40 eV and the scan time was set to 0.2 s. The MS data were acquired in centroid MSE mode and identified according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Metabolites differentially expressed between the sugar treatments and the control were selected based on the parameters fold-change (sugar treatment/control) > 1.2 or < 0.8333 [42].…”

Section: Metabolomics Analysismentioning

confidence: 99%

Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro

Wang

Cao

Wang

et al. 2022

Plants

View full text Add to dashboard Cite

Anthocyanins are important pigments in peach fruit and are beneficial to human health. Sugars are both energy-storing and signaling molecules and their roles in inducing anthocyanin biosynthesis have received a great deal of research attention. However, the mechanism by which sugars induce anthocyanin biosynthesis in peach fruit is unknown. In order to understand this induction mechanism, comprehensive transcriptome and metabolome were performed in fruit flesh treated with four different sugars for 12 and 24 h, respectively. Here, we found that cyanidin-3-O-(6-O-p-coumaroyl) glucosides accumulated in fruit flesh treated with glucose, sucrose, sorbitol, and fructose in vitro. Two key structural genes of the anthocyanin biosynthesis pathway, namely, PpDFR and PpUFGT, were upregulated in the flesh of sugar-treated peach fruit. By contrast, the two main transcription factors (TFs) PpMYB10.1 and PpBL regulating anthocyanin biosynthetic genes in peach fruit were not upregulated accordingly. Interestingly, two MYB family genes (PpMYB6 and PpMYB44-like) and three bHLH family genes (PpbHLH35, PpbHLH51, and PpbHLH36-like) were upregulated. A dual luciferase assay revealed that PpMYB6 strongly activated the PpUFGT promoter when it was co-infiltrated with PpbHLH35, PpbHLH51, and PpbHLH36-like. When PpMYB44-like was co-infiltrated with PpbHLH35, it also potently activated the PpUFGT promoter. The results of this study help clarify the molecular mechanisms by which glucose, sucrose, sorbitol, and fructose regulate anthocyanin accumulation in peach fruit.

show abstract

Section: Metabolomics Analysismentioning

confidence: 99%

Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro

Wang

Cao

Wang

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

“…The phenylpropanoid pathway is one of the most important pathways associated with plant secondary metabolism and includes two important branches: One branch regulates cinnamaldehyde and coumarine synthesis, whereas the other branch regulates flavonoid metabolism. The research identified some structural genes that may influence the biosynthesis of cinnamaldehyde, coumarine, and flavonoids in C. cassia ( Gao, Xu, Zhang, Qian, & Quan, 2020 ). However, there has not been a comprehensive genetic study on the impact of these isoforms on phenylpropanoid metabolism in C. cassia .…”

Section: Introductionmentioning

confidence: 99%

De novo transcriptome assembly and metabolomic analysis of three tissue types in Cinnamomum cassia

Gao

Zhang

et al. 2023

Chinese Herbal Medicines

Self Cite

View full text Add to dashboard Cite

“…In most plants, photosynthesis has an impact on specialized metabolite synthesis. The production of alkaloids is influenced by photosynthesis [19,20].…”

Section: Comparative Functional Annotation Of Identified Degs In Bfc ...mentioning

confidence: 99%

“…3 and Table S4). On the other hand, the genes reported to be involved in the MEP pathway in other plants [20], including Cluster-47351.0 (predicted to be 1-deoxy-Dxylulose-5-phosphate synthase, DXS), Cluster-47351.0 (predicted to be 1-deoxy-D-xylulose-5-phosphatereducto-isomerase, DXR), Cluster-1034.20772 (predicted to be 2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase, MCT), Cluster-1034.34336 (predicted to be 4-(cytidine 5-diphospho) -2-C-methyl-Derythritolkinase, CMK), Cluster-1034.12325(predicted to be 2-C-methyl-erythritol 2,4-cyclodiphosphatesynthase, MCS), Cluster-1034.9543 (predicted to be 1-hydroxy-2methyl-2-(E)-butenyl-4-diphosphate synthase, HDS), and Cluster-1034.31766 (predicted to be 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase, HDR) were found to be expressed higher in BFT than that of in BFC. These findings suggested that the MVA pathway and MEP pathway are likely to be responsible for the biosynthesis of the key intermediate of steroidal alkaloids in BFC and BFT, respectively.…”

Section: Analysis and Identification Of Candidate Genes Possibly Invo...mentioning

confidence: 99%

Integrative analysis of the steroidal alkaloids distribution and biosynthesis of bulbs Fritillariae Cirrhosae through metabolome and transcriptome analyses

Liao

et al. 2022

BMC Genomics

View full text Add to dashboard Cite

Background Bulbus Fritillariae Cirrhosae (BFC) is an endangered high-altitude medicine and food homology plant with anti-tumor, anti-asthmatic, and antitussive activities as it contains a variety of active ingredients, especially steroidal alkaloids. Bulbus Fritillariae Thunbergia (BFT) is another species of Fritillaria that grows at lower altitude areas. Production of plant-derived active ingredients through a synthetic biology strategy is one of the current hot topics in biological research, which requires a complete understanding of the related molecular pathways. Our knowledge of the steroidal alkaloid biosynthesis in Fritillaria species is still very limited. Results To promote our understanding of these pathways, we performed non-target metabolomics and transcriptome analysis of BFC and BFT. Metabolomics analysis identified 1288 metabolites in BFC and BFT in total. Steroidal alkaloids, including the proposed active ingredients of Fritillaria species peimine, peimisine, peiminine, etc., were the most abundant alkaloids detected. Our metabolomics data also showed that the contents of the majority of the steroidal alkaloids in BFC were higher than in BFT. Further, our comparative transcriptome analyses between BFC and BFT identified differentially expressed gene sets among these species, which are potentially involved in the alkaloids biosynthesis of BFC. Conclusion These findings promote our understanding of the mechanism of steroidal alkaloids biosynthesis in Fritillaria species.

show abstract

Transcriptomics and metabolomics analyses reveal the differential accumulation of phenylpropanoids between Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu

Cited by 17 publications

References 41 publications

Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro

Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro

De novo transcriptome assembly and metabolomic analysis of three tissue types in Cinnamomum cassia

Integrative analysis of the steroidal alkaloids distribution and biosynthesis of bulbs Fritillariae Cirrhosae through metabolome and transcriptome analyses

Contact Info

Product

Resources

About