Transcriptomics changes and the candidate pathway in human macrophages induced by different PM2.5 extracts

An, Jing; Tang, Waner; Wang, Lu; Xue, Wanlei; Yao, Weiwei; Zhong, Yufang; Qiu, Xinghua; Li, Yang; Chen, Yingjun; Wang, Hongli; Shang, Yu

doi:10.1016/j.envpol.2021.117890

Cited by 17 publications

(7 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The research similar to this study applied GO and KEGG to analyze changes in genetic and signal cascades during PM exposure in macrophage. Results revealed that C-X-C motif chemokine ligand and interleukin family members were the nodal genes that cause oxidative stress and inflammatory responses [ 32 ]. Furthermore, Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The expression of each gene was calculated using feature counts and referred from the Mouse Reference Genome ( Mus musculus )-GRCm38 gene database. Differential expression genes (DEGs) between each treatment group were screened by DESeq2 with biological repeatability, with the values set to log2 fold change ≥2 and p -value adjusted to ≥0.05 for significant difference in Gene Ontology (GO) analysis, as described by An et al (2021) [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Glycine-rich peptides from fermented Chenopodium formosanum sprout as an antioxidant to modulate the oxidative stress

Hsieh,

Yu,

Kuo

et al. 2023

Journal of Food and Drug Analysis

View full text Add to dashboard Cite

Rhizopus oligosporus was utilized in the solid-state fermentation of Chenopodiumformosanum sprouts (FCS) in a bioreactor. Subsequently, the antioxidant activity of food proteins derived from FCS was investigated. Results showed that glycine-rich peptide (GGGGGKP, G-rich peptide), identified from the <2 kDa FCS proteins, had antioxidant values. According to SwissADME, AllerTOP, ToxinPred, and BIOPEP-UWM analyses, G-rich peptide was identified as safe, non-toxic, and non-allergenic. Afterward, the peptide was examined using in silico and in vitro studies to evaluate its potential alleviating oxidative stress caused by particulate matter. This study proposed plausible mechanisms that involve the binding of G-rich peptide which inhibited phosphorylation of the v-rel avian reticuloendotheliosis viral oncogene homologA(RELA) subunit onNF-κB pathway. The inhibition then resulted in down regulation of NF-κB transcription and genetic expression of inflammatory responses. These findings suggested that G-rich peptide from FCS proteins can potentially alleviate oxidative stress.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Glycine-rich peptides from fermented Chenopodium formosanum sprout as an antioxidant to modulate the oxidative stress

Hsieh,

Yu,

Kuo

et al. 2023

Journal of Food and Drug Analysis

View full text Add to dashboard Cite

show abstract

“…The most highly changed terms in these clusters were related to the cell cycle and DNA synthesis, namely, cell cycle, p53 signaling pathway, oocyte meiosis, DNA replication and cellular senescence. In reports by other authors, as a result of 12 h of exposure of human monocytic THP-1-derived macrophages to PM collected in Beijing, China, the same terms were also among the most highly altered [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

“…Examples include studies in which exposure to PM from different sources and with different chemical compositions (e.g., the NIST164b standard or PM collected in Taiyuan in 2017–2018) caused different changes in gene expression [ 69 , 72 ]. Moreover, different extracts of the same PM induced slightly different patterns of gene expression: the aqueous extract induced greater gene changes in molecular pathways related to oxidative stress and inflammation and the organic extract had a greater effect on genes and signaling pathways related to the cell cycle [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Changes Induced by Exposure of RAW 264.7 Macrophages to Particulate Matter of Air Pollution: The Role of Endotoxins

et al. 2022

View full text Add to dashboard Cite

Despite the variable chemical and physical characteristics of particulate air pollutants, inflammation and oxidative stress have been identified as common mechanisms for cell damage and negative health influences. These effects are produced by organic components, especially by endotoxins. This study analyzed the gene expression profile after exposure of RAW 264.7 cells to the standard particulate matter (PM) material, NIST1648a, and PM with a reduced organic matter content, LAp120, in comparison to the effects of lipopolysaccharide (LPS). The selected parameters of cell viability, cell cycle progression, and metabolic and inflammatory activity were also investigated. Both forms of PM negatively influenced the parameters of cell activity. These results were generally reflected in the gene expression profile. Only NIST1648a, excluding LAp120, contained endotoxins and showed small but statistically significant pro-inflammatory activity. However, the gene expression profiling revealed strong pro-inflammatory cell activation induced by NIST1648a that was close to the effects of LPS. Changes in gene expression triggered by LAp120 were relatively small. The observed differences in the effects of NIST1648a and LAp120 were related to the content of organic matter in which bacterial endotoxins play an important role. However, other organic compounds and their interactions with other PM components also appear to be of significant importance.

show abstract

“…Another possible explanation is the osmotic pressure changes in the cell membrane caused by air pollution. Studies have reported that the organic components of PM 2.5 may contribute to cell cycle dysregulation as well as several other responses (ie, elevated osmotic pressure and exacerbation of cell inflammation) 36. Additionally, the toxic effects of fine particle exposure may increase the vascular permeability of endothelial cells and may further cause cell swelling because of the excess fluid in the intracellular environment 37.…”

Section: Discussionmentioning

confidence: 99%

Association between air pollutant exposure, body water distribution and sleep disorder indices in individuals with low-arousal-threshold obstructive sleep apnoea

Tsai,

Liu,

Huang

et al. 2023

BMJ Open Resp Res

View full text Add to dashboard Cite

BackgroundAir pollution may alter body water distribution, it may also be linked to low-arousal-threshold obstructive sleep apnoea (low-ArTH OSA). Here, we explored the mediation effects of air pollution on body water distribution and low-ArTH OSA manifestations.MethodsIn this retrospective study, we obtained sleep centre data from healthy participants and patients with low-ArTH OSA (N=1924) in northern Taiwan. Air pollutant exposure at different time intervals (1, 3, 6 and 12 months) was estimated using the nearest station estimation method, and government air-quality data were also obtained. Regression models were used to assess the associations of estimated exposure, sleep disorder indices and body water distribution with the risk of low-ArTH OSA. Mediation analysis was performed to explore the relationships between air pollution, body water distribution and sleep disorder indices.ResultsFirst, exposure to particulate matter (PM) with a diameter of ≤10 µm (PM10) for 1 and 3 months and exposure to PM with a diameter of ≤2.5 µm (PM2.5) for 3 months were significantly associated with the Apnoea–Hypopnoea Index (AHI), Oxygen Desaturation Index (ODI), Arousal Index (ArI) and intracellular-to-extracellular water ratio (I-E water ratio). Significant associations were observed between the risk of low-ArTH OSA and 1- month exposure to PM10(OR 1.42, 95% CI 1.09 to 1.84), PM2.5(OR 1.33, 95% CI 1.02 to 1.74) and ozone (OR 1.27, 95% CI 1.01 to 1.6). I-E water ratio alternation caused by 1-month exposure to PM10and 3-month exposure to PM2.5and PM10had partial mediation effects on AHI and ODI.ConclusionAir pollution can directly increase sleep disorder indices (AHI, ODI and ArI) and alter body water distribution, thus mediating the risk of low-ArTH OSA.

show abstract

Transcriptomics changes and the candidate pathway in human macrophages induced by different PM2.5 extracts

Cited by 17 publications

References 47 publications

Glycine-rich peptides from fermented Chenopodium formosanum sprout as an antioxidant to modulate the oxidative stress

Glycine-rich peptides from fermented Chenopodium formosanum sprout as an antioxidant to modulate the oxidative stress

Gene Expression Changes Induced by Exposure of RAW 264.7 Macrophages to Particulate Matter of Air Pollution: The Role of Endotoxins

Association between air pollutant exposure, body water distribution and sleep disorder indices in individuals with low-arousal-threshold obstructive sleep apnoea

Contact Info

Product

Resources

About